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Recombinant Human u-Plasminogen Activator/Urokinase, CF

Bio-Techne includes R&D Systems | Catalog # 1310-SE

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1310-SE-010

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Enzyme Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human u-Plasminogen Activator (uPA)/Urokinase protein
Met1-Leu431 with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser21, Ile179 & Lys156

Predicted Molecular Mass

18 kDa (long A chain), 3 kDa (short A chain), 30 kDa (B chain)

SDS-PAGE

18 kDa and 32 kDa, reducing conditions

Activity

Measured by its ability to cleave a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC).
The specific activity is >2,000 pmol/min/µg, as measured under the described conditions.

Scientific Data Images for Recombinant Human u-Plasminogen Activator/Urokinase, CF

Recombinant Human u-Plasminogen Activator/Urokinase Enzyme Activity

Recombinant Human u-Plasminogen Activator/Urokinase Enzyme Activity

Recombinant Human u-Plasminogen Activator/Urokinase (Catalog # 1310-SE) is measured by its ability to cleave a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC).

Formulation, Preparation and Storage

1310-SE
Formulation Supplied as a 0.2 μm filtered solution in HEPES, NaCl and CaCl2.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: u-Plasminogen Activator (uPA)/Urokinase

uPA is a serine protease with an extremely limited substrate specificity, cleaving the sequence Cys-Pro-Gly-Arg560-Val561-Val-Gly-Gly-Cys in plasminogen to form plasmin (1). uPA is a potent marker of invasion and metastasis in a variety of human cancers associated with breast, stomach, colon, bladder, ovary, brain and endometrium (2). For example, the combination (both low vs. either or both high) of uPA and its inhibitor, plasminogen activator inhibitor-1 (PAI-1), outperforms the single factors as well as other traditional prognostic factors with regard to risk group assessment for breast cancer, particularly in node-negative breast cancer (3). The human uPA is initially synthesized as 431 amino acid precursor with a N-terminal signal peptide (20 residues) (4‑6). The single chain molecule is processed into a disulfide-linked two-chain molecule. The B chain starting at Ile179 corresponds to the catalytic domain. Two forms of the A chain exist, one starting at Ser21 (the long form) and the other at Lys156 (the short form). The resulting two-chain forms have different molecular weights (MW). The B chain is common for both forms whereas the long and short A chains are unique to the high and low MW forms, respectively. The long A chain contains an EGF-like domain, which is responsible for binding of the uPA receptor (uPAR). Both high and low MW forms exist in the purified recombinant human uPA.

References

  1. Ellis, V. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. eds., Academic Press, San Diego, pp.1677.
  2. Duffy, M.J. (2002) Biochem. Soc. Trans. 30:207.
  3. Harbeck, N. et al. (2002) Clin. Breast Cancer 3:196.
  4. Riccio, A. et al. (1985) Nucleic Acids Res. 13:2785.
  5. Nagai, M. et al. (1985) Gene 36:183.
  6. Jacobs, P. et al. (1985) DNA 4:139.

Long Name

Urokinase-type Plasminogen Activator

Alternate Names

PLAU, uPA, uPlasminogen Activator, Urokinase

Entrez Gene IDs

5328 (Human); 18792 (Mouse); 102135886 (Cynomolgus Monkey)

Gene Symbol

PLAU

UniProt

Additional u-Plasminogen Activator (uPA)/Urokinase Products

Product Documents for Recombinant Human u-Plasminogen Activator/Urokinase, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human u-Plasminogen Activator/Urokinase, CF

For research use only

Citations for Recombinant Human u-Plasminogen Activator/Urokinase, CF (12)

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FAQs for Recombinant Human u-Plasminogen Activator/Urokinase, CF

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  • Q: Has R&D Systems examined the binding ability of 1310-SE to uPAR?

    A: In house, we measured the bioactivity of 1310-SE based on its ability to cleave the Z-GGR-AMC peptide substrate. While we did not measure the binding ability in this assay, we do offer the Recombinant Human uPAR Protein (Catalog # 807-UK), which has been tested in a functional ELISA binding assay with 1310-SE. We have found that the 1310-SE protein is able to bind to uPAR through this testing.

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