Human S100A8/S100A9 Heterodimer Antibody

Name: Human S100A8/S100A9 Heterodimer Antibody
Brand: R&D Systems
SKU: MAB45703
Price: 111 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB45703

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Species Reactivity:	Human
Immunogen:	E. coli-derived recombinant human S100A8/S100A9 heterodimer Met1-Glu93 (S100A8) & Thr2-Pro114 (S100A9) Accession # P05109 (S100A8) and P06702 (S100A9)
Applications:	CyTOF-ready, ELISA, Flow Cytometry
Label:	Unconjugated
Source:	Monoclonal Mouse IgG_2A Clone # 900028
Specificity:	Detects human S100A8/S100A9 heterodimer in direct ELISAs. In direct ELISAs, no detection of recombinant human S100A8 or S100A9 monomers was observered.

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Applications for Human S100A8/S100A9 Heterodimer Antibody

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human S100A8/S100A9 Heterodimer Monoclonal Antibody (Catalog # MAB45702).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human S100A8/S100A9 Heterodimer DuoSet ELISA Kit (Catalog # ) for convenient development of a sandwich ELISA or the Human S100A8/S100A9 Heterodimer Quantikine ELISA Kit (Catalog # ) for a complete optimized ELISA.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells

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Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human S100A8/S100A9 Heterodimer Antibody

	Intracellular Staining by Flow Cytometry Detection of S100A8/A9 in Human PBMC Monocytes by Flow Cytometry Human peripheral blood monocytes (PBMC) were stained with Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Mouse Anti-Human S100A8/A9 Monoclonal Antibody (Catalog # MAB45703) or (B) Mouse IgG2A Isotype Control (Catalog # MAB003) followed by Goat anti-Mouse IgG APC-conjugated Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Formulation, Preparation and Storage

Purification	Protein A or G purified from cell culture supernatant
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: S100A8/S100A9 Heterodimer

S100A8 (also known as MRP8, Calgranulin A, and CP-10) and S100A9 (also known as MRP14 and Calgranulin B) are pro-inflammatory members of the S100 family of secreted calcium binding proteins (1, 2). They are up-regulated in neutrophils and monocytes at sites of inflammation (e.g. psoriasis, rheumatoid arthritis, cardiac ischemia) and are present at elevated concentrations in rheumatoid arthritis synovial fluid (3-5). The 10 kDa human S100A8 and 14 kDa S100A9 each contain two EF‑hand calcium binding motifs. Human S100A8 shares 57% and 61% amino acid (aa) sequence identity with mouse and rat S100A8, respectively. Human S100A9 shares 57% and 62% amino acid sequence identity with mouse and rat S100A9, respectively (6, 7). In the presence of calcium or zinc, S100A8 and S100A9 associate into non-covalent homodimers and 34-35 kDa heterodimers with each other (8-10). The heterodimer additionally binds and sequesters manganese, thereby restricting the growth of Mn-dependent bacteria (11). The S100A8/A9 heterodimer exhibits functions beyond those performed by the individual proteins. These include binding to fatty acids such as arachidonic acid and promoting astrocyte proliferation (3, 12). S100A8, S100A9, and the heterodimer each promote neutrophil infiltration into sites of inflammation and inflammatory cytokine production by monocytes (4, 5, 9).

Item	Value
References	Averill, M.M. et al. (2012) Arterioscler. Thromb. Vasc. Biol. 32:223. Vogl, T. et al. (2012) Int. J. Mol. Sci. 13:2893. Siegenthaler, G. et al. (1997) J. Biol. Chem. 272:9371. Sunahori, K. et al. (2006) Arthritis Res. Ther. 8:R69. Volz, H.C. et al. (2012) Basic Res. Cardiol. 107:250. Odink, K. et al. (1987) Nature 330:80. Dorin, J.R. et al. (1987) Nature 326:614. Teigelkamp, S. et al. (1991) J. Biol. Chem. 266:13462. Ryckman, C. et al. (2003) J. Immunol. 170:3233. Vogl, T. et al. (2006) Biochim. Biophys. Acta 1763:1298. Damo, S.M. et al. (2013) Proc. Natl. Acad. Sci. USA 110:3841. Ryu, M-J. et al. (2012) J. Biol. Chem. 287:22948.
Long Name	S100 Calcium Binding Protein A8/S100 Calcium Binding Protein A9
Entrez Gene IDs	6279 (Human)
Alternate Names	Calprotectin, S100A8/S100A9 Heterodimer

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Human S100A8/S100A9 Heterodimer Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Human S100A8/S100A9 Heterodimer Antibody

Scientific Data Examples for Human S100A8/S100A9 Heterodimer Antibody

Intracellular Staining by Flow Cytometry

Formulation, Preparation and Storage

Reconstitution Calculator

Background: S100A8/S100A9 Heterodimer

Citations for Human S100A8/S100A9 Heterodimer Antibody

Product Documents for Human S100A8/S100A9 Heterodimer Antibody

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