Human S100A8/S100A9 Heterodimer Quantikine ELISA Kit Best Seller

Name: Human S100A8/S100A9 Heterodimer Quantikine ELISA Kit
Brand: R&D Systems
SKU: DS8900
Rating: 5 (2 reviews)

Catalog # DS8900 | R&D Systems, Inc. a Bio-Techne Brand

(2)

Citations (15)

Product Datasheet / COA / SDS

Setting the standard in quality research reagents

Key Product Details

Assay Length:	4.5 hours
Sample Type & Volume Required Per Well:	Cell Culture Supernates (50 uL), Tissue Lysates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (10 uL), Urine (50 uL), Human Milk (10 uL), Fecal Extract (50 uL)
Sensitivity:	0.215 ng/mL
Assay Range:	0.625 - 40 ng/mL (Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk)
Human S100A8/S100A9 Heterodimer Quantikine ELISA Kit Summary:	The Quantikine Human S100A8/S100A9 Heterodimer Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human S100A8/S100A9 Heterodimer in cell culture supernates, tissue lysastes, serum, plasma, saliva, urine, human milk, and fecal extract. It contains E. coli-expressed recombinant human S100A8/S100A9 Heterodimer and antibodies raised against the recombinant factor. Results obtained using natural human S100A8/S100A9 Heterodimer showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human S100A8/S100A9 Heterodimer.

View Full Technical Details for Human S100A8/S100A9 Heterodimer Quantikine ELISA Kit

Technical Data
Citations (15) / Reviews (2)

Product Specifications for Human S100A8/S100A9 Heterodimer Quantikine ELISA Kit

Measurement	Quantitative ELISA
Detection Method	Colorimetric - 450nm (TMB)
Conjugate	HRP
Reactivity	Human
Specificity	Natural and recombinant human S100A8/S100A9 Heterodimer.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	5.64	12.1	25.3	6.09	12.6	24.6
Standard Deviation	0.155	0.37	1.14	0.352	0.606	0.792
CV%	2.7	3.1	4.5	5.8	4.8	3.2

Recovery

The recovery of human S100A8/S100A9 Heterodimer spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	85	79-96
Cell Lysis Buffer (n=3)	94	80-104
Human Milk (n=4)	96	83-103
Urine (n=4)	100	91-113

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human S100A8/S100A9 Heterodimer were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Scientific Data Examples for Human S100A8/S100A9 Heterodimer Quantikine ELISA Kit

	Human S100A8/S100A9 Heterodimer ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: S100A8/S100A9 Heterodimer

S100A8 (also MRP8 and calgranulin A) is a 10 kDa member of the S100 family, EF-hand superfamily of Ca-binding proteins. It is produced by neutrophils and monocytes, and forms Ca²⁺-dependent heterodimer/heterotetramer complexes (termed calprotectin) with S100A9. It functions both intracellularly and extracellularly, where it binds to RAGE and CD36. Human S100A8 is 93 amino acids (aa) in length. It contains two EF-hand motifs (aa 12 - 47 and 46 - 81) and one high-affinity Ca²⁺-binding site (aa 59 - 70). There may be one splice form that shows a 15 aa substitution for the C-terminal 14 amino acids. Although mouse S100A8 is cleaved by MMP-2 after Asn21, it is unclear if human S100A8 is susceptible. Full-length human S100A8 is 57% and 74% aa identical to mouse and canine S100A8, respectively.

Item	Value
Long Name	S100 Calcium Binding Protein A8/S100 Calcium Binding Protein A9
Entrez Gene IDs	6279 (Human)
Alternate Names	Calprotectin, S100A8/S100A9 Heterodimer

Citations for Human S100A8/S100A9 Heterodimer Quantikine ELISA Kit (15)

Showing 1 - 10 of 15 citations Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Human S100A8/S100A9 Heterodimer Quantikine ELISA Kit include:

Species: Human
Sample Types: Serum

Pons et al. (2023-06-13), Performance of 11 Host Biomarkers Alone or in Combination in the Diagnosis of Late-Onset Sepsis in Hospitalized Neonates: The Prospective EMERAUDE Study Biomedicines
PMID: 37371798
Species: Human
Sample Types: Tissue Homogenates

F Real et al. (2022-10-11), S100A8-mediated metabolic adaptation controls HIV-1 persistence in macrophages in vivo Nature Communications, 2022-113(1):5956.
PMID: 36220814
Species: Human
Sample Types: Plasma

Z Li et al. (2022-04-19), ESR1 mutant breast cancers show elevated basal cytokeratins and immune activation Nature Communications, 2022-013(1):2011.
PMID: 35440136
Species: Human
Sample Types: Plasma

AV Sen'kova et al. (2021-11-22), Core genes involved in the regulation of acute lung injury and their association with COVID-19 and tumor progression: A bioinformatics and experimental study PLoS ONE, 2021-116(11):e0260450.
PMID: 34807957
Species: Human
Sample Types: Stool

GA Kinney et al. (2021-10-06), An Intervention With Michigan-Grown Wheat in Healthy Adult Humans to Determine Effect on Gut Microbiota: Protocol for a Crossover Trial JMIR research protocols, 2021-110(10):e29046.
PMID: 34612840
Species: Human
Sample Types: Plasma

IA Drosatos et al. (2021-09-13), Differential Regulation of Circulating Soluble Receptor for Advanced Glycation End Products (sRAGEs) and Its Ligands S100A8/A9 Four Weeks Post an Exercise Intervention in a Cohort of Young Army Recruits Biomolecules, 2021-011(9).
PMID: 34572568
Species: Human
Sample Types: Serum

S Buhelt et al. (2021-07-07), Biomarkers of systemic inflammation, soluble IL-2Ralpha and the multiple sclerosis-associated IL2RA SNP rs2104286 in healthy subjects and multiple sclerosis patients Multiple sclerosis and related disorders, 2021-054(0):103140.
PMID: 34304016
Species: Human
Sample Types: Serum

A Olsson et al. (2021-07-03), Circulating levels of tight junction proteins in multiple sclerosis: Association with inflammation and disease activity before and after disease modifying therapy Multiple sclerosis and related disorders, 2021-054(0):103136.
PMID: 34247104
Species: Human
Sample Types: Serum

A Olsson et al. (2020-09-19), Biomarkers of inflammation and epithelial barrier function in multiple sclerosis Mult Scler Relat Disord, 2020-046(0):102520.
PMID: 32980645
Species: Human
Sample Types: Cell Culture Supernates

DG Bisson et al. (2020-08-27), Toll-like receptor involvement in adolescent scoliotic facet joint degeneration J. Cell. Mol. Med., 2020-00(0).
PMID: 32853438
Species: Human
Sample Types: Serum

DR Shores et al. (2020-05-26), Normative values for circulating intestinal fatty acid binding protein and calprotectin across gestational ages BMC Pediatr, 2020-020(1):250.
PMID: 32456678
Species: Human
Sample Types: Plasma

A Grand et al. (2020-03-20), Body Mass Index and Calprotectin Blood Level Correlation in Healthy Children: An Individual Patient Data Meta-Analysis J Clin Med, 2020-09(3).
PMID: 32245056
Species: Human
Sample Types: Plasma

RD Williamson et al. (2019-04-11), Activation of a TLR9 mediated innate immune response in preeclampsia Sci Rep, 2019-09(1):5920.
PMID: 30976066
Species: Human

CJ Popp et al. (2019-03-04), The rationale and design of the personal diet study, a randomized clinical trial evaluating a personalized approach to weight loss in individuals with pre-diabetes and early-stage type 2 diabetes Contemp Clin Trials, 2019-00(0).
PMID: 30844471
Species: Human
Sample Types: Serum

(2016-09-26), Dietary zinc alters the microbiota and decreases resistance to Clostridium difficile infection Nat Med, 2016-00(0).
PMID: 27668938

There are no citations that match your criteria.

Showing 1 - 10 of 15 citations Showing All

Customer Reviews for Human S100A8/S100A9 Heterodimer Quantikine ELISA Kit (2)

4.5 out of 5

2 customer ratings

5 stars

50%

4 stars

50%

3 stars

2 stars

1 star

Customer Images

Showing 1 - 2 of 2 reviews Showing All

Filter By:

Human S100A8/S100A9 Heterodimer Quantikine ELISA Kit

Name: Mike Ehrhardt

Sample Tested: EDTA Plasma

Verified Customer | Posted 11/24/2020

This ELISA performed as expected with low % CV's
Human S100A8/S100A9 Heterodimer Quantikine ELISA Kit

Name: Anonymous

Sample Tested: Human cells

Verified Customer | Posted 09/07/2017

There are no reviews that match your criteria.

Showing 1 - 2 of 2 reviews Showing All

Product Documents for Human S100A8/S100A9 Heterodimer Quantikine ELISA Kit

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.