Human PlGF Biotinylated Antibody

Catalog # BAF264 | R&D Systems, Inc. a Bio-Techne Brand
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Key Product Details

Species Reactivity

Human

Applications

ELISA Detection (Matched Antibody Pair), Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG

Product Summary for Human PlGF Biotinylated Antibody

Immunogen

E. coli-derived recombinant human PlGF
Ala21-Arg149
Accession # CAA38698

Specificity

Detects human PlGF in ELISAs and Western blots. In sandwich immunoassays, less than 20% cross-reactivity with recombinant human (rh) VEGF/PlGF is observed and less than 0.07% cross-reactivity with rhFlt-1, recombinant mouse PlGF-2, rhPDGF, and rhVEGF is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human PlGF Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human PlGF (Catalog # 264-PGB)

Human PlGF Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human PlGF Antibody (Catalog # MAB264R)
  • Standard: Recombinant Human PlGF Protein (Catalog # 264-PGB)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using BAF264 in the following applications:

Published Applications

Read 2 publications using BAF264 in the following applications:

Formulation, Preparation and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PlGF

Placenta growth factor (PlGF) is a member of the vascular endothelial growth factor (VEGF) family of growth factors. PlGF and VEGF share primary structural as well as limited amino acid sequence homology with the A and B chains of PDGF. All eight cysteine residues involved in intra- and inter-chain disulfides are conserved among these growth factors. In their PDGF-like regions, VEGF and PlGF also share approximately 53% amino acid sequence similarity. The gene for PlGF has been mapped to chromosome 14. As a result of alternative splicing, at least two PlGF mRNAs encoding monomeric PlGF precursors containing 149 and 170 amino acid residues have been described. The expression of PlGF is not widespread, but has been detected in human umbilical vein endothelial cells, placenta, choriocarcinoma cell lines, and in renal cell carcinoma associated with angiogenesis. The PlGF proteins bind with high-affinity to Flt-1, but not to Flk-1/KDR.

Long Name

Placenta Growth Factor

Alternate Names

PGF, PGFL

Entrez Gene IDs

5228 (Human); 18654 (Mouse)

Gene Symbol

PGF

UniProt

CAA38698

Product Documents for Human PlGF Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human PlGF Biotinylated Antibody

For research use only

Citations for Human PlGF Biotinylated Antibody (2)

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Customer Reviews for Human PlGF Biotinylated Antibody (1)

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  • Human PlGF Biotinylated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: U87
    Species: Human
    Verified Customer | Posted 02/17/2021
    Quantification of the amount of PlGF in the conditioned medium was performed using anti-PlGF IgGs at a concentration of 10 μg/ml in PBS, to coat Maxisorp Nunc immunoplates
    Human PlGF Biotinylated Antibody BAF264

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Reconstitution Calculator

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