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Human PlGF Antibody

Catalog # MAB264 | R&D Systems, Inc. a Bio-Techne Brand
Catalog #
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Key Product Details

Species Reactivity



ELISA Capture (Matched Antibody Pair)



Antibody Source

Monoclonal Mouse IgG1 Clone # 37203

Product Summary for Human PlGF Antibody


E. coli-derived recombinant human PlGF
Accession # P49763


Detects human PlGF in ELISAs. In ELISAs, this antibody does not cross-react with recombinant human (rh) VEGF, rhPDGF‑AA, rhPDGF‑AB, or rhPDGF‑BB.







Applications for Human PlGF Antibody

Recommended Usage

Human PlGF Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human PlGF Biotinylated Antibody (Catalog # BAF264)
  • Standard: Recombinant Human PlGF Protein (Catalog # 264-PGB)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using MAB264 in the following applications:

Published Applications

Read 4 publications using MAB264 in the following applications:

Formulation, Preparation and Storage


Protein A or G purified from hybridoma culture supernatant


Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price


Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PlGF

Placenta growth factor (PlGF) is a member of the vascular endothelial growth factor (VEGF) family of growth factors. As a result of alternative splicing, at least two PlGF mRNAs encoding monomeric PlGF precursors containing 149 and 170 amino acid residues have been described. The expression of PlGF is not widespread but has been detected in human umbilical vein endothelial cells, placenta, choriocarcinoma cell lines, and in renal cell carcinoma associated with angiogenesis. The PlGF proteins bind with high-affinity to VEGF R1/Flt-1 but not to VEGF R2/Flk-1.

Long Name

Placenta Growth Factor

Alternate Names


Entrez Gene IDs

5228 (Human); 18654 (Mouse)

Gene Symbol



Product Documents for Human PlGF Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human PlGF Antibody

For research use only

Citations for Human PlGF Antibody (4)

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Customer Reviews for Human PlGF Antibody (2)

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  • Human PlGF Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Endothelial colony-forming cells
    Species: Mouse
    Verified Customer | Posted 02/17/2021
    Cells were fixed in 4% paraformaldehyde for 20 min at room temperature. Following PBS washes, cells were permeabilised with 0.1% Triton X-100 for 10 min at RT and then blocked in 1% bovine serum albumin for 2 h in PBS before overnight incubation in primary antibody at 4 °C. After washing with PBS, cells were incubated with appropriate secondary antibody for 1 h at RT and imaged
    Human PlGF Antibody MAB264
  • Human PlGF Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Placental tissue
    Species: Human
    Verified Customer | Posted 11/26/2020
    Human placenta were fixed in 4% paraformaldehyde. Tissue was embedded and 5 micron sections cut. Sections were permeabilized with PBS containing 0.1% Triton X-100, then blocked for 30 min in 3% BSA in PBS containing 0.1% Triton X-100. Goat Anti-Human PIGF Antigen was diluted in blocking buffer to 1:200 and applied to sections for 1 hour at RT. Sections were washed 3X with PBS containing 0.1% Triton X-100, then incubated with secondary antibody.
    Human PlGF Antibody MAB264

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Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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