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Human PlGF Antibody
Catalog # MAB264 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Human
Applications
ELISA Capture (Matched Antibody Pair)
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 37203
Product Summary for Human PlGF Antibody
Immunogen
E. coli-derived recombinant human PlGF
Ala21-Arg149
Accession # P49763
Ala21-Arg149
Accession # P49763
Specificity
Detects human PlGF in ELISAs. In ELISAs, this antibody does not cross-react with recombinant human (rh) VEGF, rhPDGF‑AA, rhPDGF‑AB, or rhPDGF‑BB.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Human PlGF Antibody
Application
Recommended Usage
Human PlGF Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 5 using MAB264 in the following applications:
Published Applications
Read 4 publications using MAB264 in the following applications:
Formulation, Preparation and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PlGF
Placenta growth factor (PlGF) is a member of the vascular endothelial growth factor (VEGF) family of growth factors. As a result of alternative splicing, at least two PlGF mRNAs encoding monomeric PlGF precursors containing 149 and 170 amino acid residues have been described. The expression of PlGF is not widespread but has been detected in human umbilical vein endothelial cells, placenta, choriocarcinoma cell lines, and in renal cell carcinoma associated with angiogenesis. The PlGF proteins bind with high-affinity to VEGF R1/Flt-1 but not to VEGF R2/Flk-1.
Product Documents for Human PlGF Antibody
Product Specific Notices for Human PlGF Antibody
For research use only
Citations for Human PlGF Antibody (4)
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Citations are publications that use Bio-Techne products. Selected citations for Human PlGF Antibody include:
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Species: Human
Sample Types: Whole Cells
Applications: Neutralization(2016-11-29), Hypoxia-induced responses by endothelial colony-forming cells are modulated by placental growth factor Stem Cell Res Ther, 2016-17(1):173.
PMID: 27899144 -
Species: Mouse
Sample Types: Matrigel Plug
Applications: NeutralizationPark et al. (2014-02-26), Interaction of mesenchymal stem cells with fibroblast-like synoviocytes via cadherin-11 promotes angiogenesis by enhanced secretion of placental growth factor. J Immunol, 2014-0192(7):3003-10.
PMID: 24574497 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationHeijink IH et al. (2006-10-05), Der p, IL-4, and TGF-beta cooperatively induce EGFR-dependent TARC expression in airway epithelium. Am. J. Respir. Cell Mol. Biol., 2006-136(3):351-9.
PMID: 17023689 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationCianfarani F et al. (2006-10-01), Placenta growth factor in diabetic wound healing: altered expression and therapeutic potential. Am. J. Pathol., 2006-1169(4):1167-82.
PMID: 17003476
There are no citations that match your criteria.
Customer Reviews for Human PlGF Antibody (2)
5 out of 5
2 customer
ratings
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Application: Immunocytochemistry/ImmunofluorescenceSample Tested: Endothelial colony-forming cellsSpecies: MouseVerified Customer | Posted 02/17/2021Cells were fixed in 4% paraformaldehyde for 20 min at room temperature. Following PBS washes, cells were permeabilised with 0.1% Triton X-100 for 10 min at RT and then blocked in 1% bovine serum albumin for 2 h in PBS before overnight incubation in primary antibody at 4 °C. After washing with PBS, cells were incubated with appropriate secondary antibody for 1 h at RT and imaged
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Application: ImmunohistochemistrySample Tested: Placental tissueSpecies: HumanVerified Customer | Posted 11/26/2020Human placenta were fixed in 4% paraformaldehyde. Tissue was embedded and 5 micron sections cut. Sections were permeabilized with PBS containing 0.1% Triton X-100, then blocked for 30 min in 3% BSA in PBS containing 0.1% Triton X-100. Goat Anti-Human PIGF Antigen was diluted in blocking buffer to 1:200 and applied to sections for 1 hour at RT. Sections were washed 3X with PBS containing 0.1% Triton X-100, then incubated with secondary antibody.
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