Human Phospho-STAT3 (S727) Antibody

Name: Human Phospho-STAT3 (S727) Antibody
Brand: R&D Systems
SKU: AF4934

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF4934

Citations (1)

Product Datasheet / COA / SDS

Catalog #

Availability

Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Species Reactivity:	Human
Immunogen:	Phosphopeptide containing human STAT3 S727 site Accession # P40763
Applications:	Western Blot
Label:	Unconjugated
Source:	Polyclonal Rabbit IgG
Specificity:	Detects human STAT3 when phosphorylated at S727 in Western blots.

View Full Technical Details for Human Phospho-STAT3 (S727) Antibody

Complete Your Research

Applications for Human Phospho-STAT3 (S727) Antibody

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human Phospho-STAT3 (S727) Antibody

	Western Blot Detection of Human Phospho-STAT3 (S727) by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IL-6 (Catalog # 206-IL) for 15 minutes. PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human Phospho-STAT3 (S727) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4934) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-STAT3 (S727) was detected at approximately 110 kDa (as indicated). For additional reference, duplicate lysates were probed with 1.0 µg/mL Human STAT3 Monoclonal Antibody (lower panel, Catalog # MAB1799). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: STAT3

PI16 (Peptidase Inhibitor 16) is a variable molecular weight (MW) member of the CRISP family of proteins. It is expressed by cardiomyocytes and serves as an autocrine negative growth regulator. Mature mouse PI16 is a type I transmembrane protein 469 amino acids (aa) in length. The extracellular region includes all but the C‑terminal 21 amino acids. A soluble form of PI16 is known. Although its predicted MW is 50 kDa, it runs anomalously at 70 kDa (unmodified) in SDS‑PAGE. With glycosylation, it appears to be 100‑110 kDa in size. Three potential variants exist. One shows a deletion of aa 51‑124, a second shows a 9 aa extension at the N‑terminus, and a third shows a deletion of aa 191‑452. Over aa 1‑464, mouse PI16 shares 86% and 61% aa identity with rat and human PI16, respectively.

Item	Value
Long Name	Signal Transducer and Activator of Transcription 3
Entrez Gene IDs	6774 (Human); 20848 (Mouse); 25125 (Rat)
Alternate Names	APRFMGC16063, Acute-phase response factor, DNA-binding protein APRF, FLJ20882, HIES, STAT3, signal transducer and activator of transcription 3, signal transducer and activator of transcription 3 (acute-phase responsefactor)

Citations for Human Phospho-STAT3 (S727) Antibody (1)

Showing 1 - 1 of 1 citation Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Human Phospho-STAT3 (S727) Antibody include:

Species: Human

Applications: Western Blot

J Saarimäki- et al. (2017), An Activating STAT3 Mutation Causes Neonatal Diabetes through Premature Induction of Pancreatic Differentiation Cell Rep, 19(2):281-294.
PMID: 28402852

There are no citations that match your criteria.

Showing 1 - 1 of 1 citation Showing All

Product Documents for Human Phospho-STAT3 (S727) Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS