Human PDGF R beta Antibody

Detection of PDGF R beta in U118MG cells by Flow Cytometry.

U118MG cells were stained with Goat Anti-Human PDGF R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF385, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.

Detection of Human PDGF R beta by Flow Cytometry

Co-expression of EC marker and PDGFR beta after EPC-CM incubation.Co-expression of vWF and PDGFR beta was measured using dual color FACS analysis. HUVEC were kept in control medium containing only 1% FCS or EPC-CM for 24 h before the measurement. HUVEC incubated in control medium showed only a fractional amount of vWF+/PDGFR beta+ cells (A). After EPC-CM exposure the proportion of vWF+/PDGFR beta+ double positive population was significantly increased, suggesting a strong phenotype shift of endothelial cells towards PDGFR beta+ (B). The addition of neutralizing antibody AF385 did not block the upreguation of the PDGFR beta by EPC-CM stimulation (C). However, such enhanced PDGFR beta expression could not be evoked by solely adding 100 ng/ml (D) or 100 pg/ml (E) rhPDGF-BB to the control medium. *, P<0.01 compared to controls. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0014107), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human PDGF R beta by Western Blot.

Western Blot shows lysates of SH-SY5Y human neuroblastoma cell line and U2OS human osteosarcoma cell line. PVDF membrane was probed with 2 µg/ml of Goat Anti-Human PDGF R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF385) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PDGF R beta at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of Mouse PDGF R beta by Immunohistochemistry

Pericyte soma on WM capillaries demonstrated by immunofluorescence. A–F. PDGFR‐ beta (red) and COL4 (green) immunofluorescence staining with nuclei (DAPI) (blue). A,B. Low‐ and high‐power images showing capillary segments (arrowheads) with overlapping PDGFR‐ beta (red), COL4 (green) and DAPI (blue). C. Same vessel segment as B with PDGFR‐ beta (red) and COL4 (green); D. COL4 (green) and DAPI (blue); E. PDGFR‐ beta (red) and DAPI (blue). F. Another capillary segment with PDGFR‐ beta (red), COL4 (green) and DAPI (blue) clearly showing pericyte cell body. G–J. Images taken by a confocal microscope showing pericyte cell bodies (arrows) with nuclear stain (DAPI). Capillaries and pericyte processes are revealed by COL4 and PDGFR‐ beta (red) immunoreactivities. Magnification bars: A = 50 µm, F, J = 10 µm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32705757), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse PDGF R beta by Immunohistochemistry

Pericyte soma on WM capillaries demonstrated by immunofluorescence. A–F. PDGFR‐ beta (red) and COL4 (green) immunofluorescence staining with nuclei (DAPI) (blue). A,B. Low‐ and high‐power images showing capillary segments (arrowheads) with overlapping PDGFR‐ beta (red), COL4 (green) and DAPI (blue). C. Same vessel segment as B with PDGFR‐ beta (red) and COL4 (green); D. COL4 (green) and DAPI (blue); E. PDGFR‐ beta (red) and DAPI (blue). F. Another capillary segment with PDGFR‐ beta (red), COL4 (green) and DAPI (blue) clearly showing pericyte cell body. G–J. Images taken by a confocal microscope showing pericyte cell bodies (arrows) with nuclear stain (DAPI). Capillaries and pericyte processes are revealed by COL4 and PDGFR‐ beta (red) immunoreactivities. Magnification bars: A = 50 µm, F, J = 10 µm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32705757), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Rhesus Macaque PDGF R beta by Immunohistochemistry

Expression of MCT8 and OATP1C1 in blood vessels and brain barriers in the human and macaque basal ganglia and adjacent choroid plexus. (A) Representative brightfield photomicrograph shows immunostaining for MCT8 in the human putamen. Note that an MCT8 immunopositive signal is observed along the capillary wall (red arrowhead), fibers (green arrowhead), and “bump-on-a-log” morphology pericytelike cells (white arrowhead). (B–H) Representative confocal microscope compositions from multiple-stained sections for MCT8 (green), the endothelial marker UEA-I (red), and the vascular and pericyte biomarker PDGFR-beta (purple) in human and macaque caudate nucleus. Merged image (E,H) shows the colocalization of all signals. (B–E) Coexpression of MCT8, UEA-I, and PDGFR-beta is observed in a vessel, while coexpression of MCT8 and PDGFR-beta but not UEA-I is observed in a capillary-associated pericyte (white arrowheads) in humans. (F–H) Coexpression of MCT8 and PDGFR-beta in a vessel and pericytelike cells (white arrowheads) in macaques. Counterstaining with DAPI (blue) shows nuclei of all cells. (I,J) Representative brightfield photomicrographs show immunostaining for MCT8 (I) and OATP1C1 (J) in the macaque choroid plexus at the lateral ventricle. Black arrowheads point to ependymocytes. Cd: caudate nucleus, Put: putamen, PDGFR-beta : platelet-derived growth factor receptor-beta, UEA-I: Ulex europaeus agglutinin-I. Scale bar = 10 μm (A–H) and 50 μm (I,J). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37298594), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human PDGF R beta by Immunohistochemistry

Expression of MCT8 and OATP1C1 in blood vessels and brain barriers in the human and macaque basal ganglia and adjacent choroid plexus. (A) Representative brightfield photomicrograph shows immunostaining for MCT8 in the human putamen. Note that an MCT8 immunopositive signal is observed along the capillary wall (red arrowhead), fibers (green arrowhead), and “bump-on-a-log” morphology pericytelike cells (white arrowhead). (B–H) Representative confocal microscope compositions from multiple-stained sections for MCT8 (green), the endothelial marker UEA-I (red), and the vascular and pericyte biomarker PDGFR-beta (purple) in human and macaque caudate nucleus. Merged image (E,H) shows the colocalization of all signals. (B–E) Coexpression of MCT8, UEA-I, and PDGFR-beta is observed in a vessel, while coexpression of MCT8 and PDGFR-beta but not UEA-I is observed in a capillary-associated pericyte (white arrowheads) in humans. (F–H) Coexpression of MCT8 and PDGFR-beta in a vessel and pericytelike cells (white arrowheads) in macaques. Counterstaining with DAPI (blue) shows nuclei of all cells. (I,J) Representative brightfield photomicrographs show immunostaining for MCT8 (I) and OATP1C1 (J) in the macaque choroid plexus at the lateral ventricle. Black arrowheads point to ependymocytes. Cd: caudate nucleus, Put: putamen, PDGFR-beta : platelet-derived growth factor receptor-beta, UEA-I: Ulex europaeus agglutinin-I. Scale bar = 10 μm (A–H) and 50 μm (I,J). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37298594), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse PDGF R beta by Immunohistochemistry

Pericyte soma on WM capillaries demonstrated by immunofluorescence. A–F. PDGFR‐ beta (red) and COL4 (green) immunofluorescence staining with nuclei (DAPI) (blue). A,B. Low‐ and high‐power images showing capillary segments (arrowheads) with overlapping PDGFR‐ beta (red), COL4 (green) and DAPI (blue). C. Same vessel segment as B with PDGFR‐ beta (red) and COL4 (green); D. COL4 (green) and DAPI (blue); E. PDGFR‐ beta (red) and DAPI (blue). F. Another capillary segment with PDGFR‐ beta (red), COL4 (green) and DAPI (blue) clearly showing pericyte cell body. G–J. Images taken by a confocal microscope showing pericyte cell bodies (arrows) with nuclear stain (DAPI). Capillaries and pericyte processes are revealed by COL4 and PDGFR‐ beta (red) immunoreactivities. Magnification bars: A = 50 µm, F, J = 10 µm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32705757), licensed under a CC-BY license. Not internally tested by R&D Systems.