Human PD‑1 Antibody
Catalog # MAB1086 | R&D Systems, Inc. a Bio-Techne Brand
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Product Summary for Human PD‑1 Antibody
Immunogen
Leu25-Gln167
Accession # Q8IX89
Specificity
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Scientific Data Images for Human PD‑1 Antibody
Detection of Human PD‑1 by Western Blot.
Western blot shows lysates of human peripheral blood mononuclear cells (PBMC) untreated (-) or treated (+) with 1 µg/mL PHA for 5 days. PVDF Membrane was probed with 1 µg/mL of Human PD-1 Monoclonal Antibody (Catalog # MAB1086) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PD-1 at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human PD‑1 Antibody
Western Blot
Sample: Human peripheral blood mononuclear cells (PBMC) treated with PHA
Reviewed Applications
Read 1 review rated 4 using MAB1086 in the following applications:
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Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PD-1
Programmed Death-1 (PD-1) is a type I transmembrane protein belonging to the CD28/CTLA-4 family of immunoreceptors that mediate signals for regulating immune responses (1). Members of the CD28/CTLA-4 family have been shown to either promote T cell activation (CD28 and ICOS) or downregulate T cell activation (CTLA-4 and PD-1) (2). PD-1 is expressed on activated T cells, B cells, myeloid cells, and on a subset of thymocytes. In vitro, ligation of PD-1 inhibits TCR-mediated T cell proliferation and production of IL-1, IL-4, IL-10, and IFN-gamma. In addition, PD-1 ligation also inhibits BCR mediated signaling. PD-1 deficient mice have a defect in peripheral tolerance and spontaneously develop autoimmune diseases (2, 3). Two B7 family proteins, PD-L1 (also called B7-H1) and PD-L2 (also known as B7-DC), have been identified as PD-1 ligands. Unlike other B7 family proteins, both PD‑L1 and PD‑L2 are expressed in a wide variety of normal tissues including heart, placenta, and activated spleens (4). The wide expression of PD-L1 and PD-L2 and the inhibitor effects on PD-1 ligation indicate that PD-1 might be involved in the regulation of peripheral tolerance and may help prevent autoimmune diseases (2). The human PD-1 gene encodes a 288 amino acid (aa) protein with a putative 20 aa signal peptide, a 148 aa extracellular region with one immunoglobulin-like V‑type domain, a 24 aa transmembrane domain, and a 95 aa cytoplasmic region. The cytoplasmic tail contains two tyrosine residues that form the immunoreceptor tyrosine-based inhibitory motif (ITIM) and immunoreceptor tyrosine-based switch motif (ITSM) that are important in mediating PD-1 signaling. Mouse and human PD-1 share approximately 60% aa sequence identity (4).
References
- Ishida, Y. et al. (1992) EMBO J. 11:3887.
- Nishimura, H. and T. Honjo (2001) Trends in Immunol. 22:265.
- Latchman, Y. et al. (2001) Nature Immun. 2:261.
- Carreno, B.M. and M. Collins (2002) Annu. Rev. Immunol. 20:29.
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Product Documents for Human PD‑1 Antibody
Product Specific Notices for Human PD‑1 Antibody
For research use only
Citations for Human PD‑1 Antibody (5)
Citations are publications that use Bio-Techne products. Selected citations for Human PD‑1 Antibody include:
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Species: Human
Sample Types: Cell Lysates
Applications: Western BlotR Motavalli et al. (2023-04-21), Evaluation of the immune checkpoint factors in idiopathic membranous nephropathy Molecular and cellular probes, 2023-069(0):101914.
PMID: 37075987 -
Species: Human
Sample Types: Whole Tissue
Applications: IHC-PR Banga et al. (2019-07-22), Lymph node migratory dendritic cells modulate HIV-1 transcription through PD-1 engagement PLoS Pathog., 2019-015(7):e1007918.
PMID: 31329640 -
Species: Human
Sample Types: Serum
Applications: ELISA DevelopmentMelendreras S et al. (2014-12-05), Soluble co-signaling molecules predict long-term graft outcome in kidney-transplanted patients. PLoS ONE, 2014-19(12):e113396.
PMID: 25478957 -
Species: Human
Sample Types: Whole Tissue
Applications: IHC-PDaridon C et al. (2012-09-06), Splenic proliferative lymphoid nodules distinct from germinal centers are sites of autoantigen stimulation in immune thrombocytopenia. Blood, 2012-0120(25):5021-31.
PMID: 22955923 -
Species: Human
Sample Types: Serum
Applications: ELISA DevelopmentSakthivel P et al. (2007-11-26), Programmed Death-1: from gene to protein in autoimmune human myasthenia gravis. J. Neuroimmunol., 2007-1193(1):149-55.
PMID: 18037500
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Customer Reviews for Human PD‑1 Antibody (1)
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Application: Western BlotSample Tested: Cancer cell lysates and HEK293 human embryonic kidney cell lineSpecies: HumanVerified Customer | Posted 07/02/2018293T cells were infected with letivirus carrying human PD-1 or control for 72h. Total cell lysates were collected and subject to Western Blot. HeLa protein sample was used as a PD-1 positive control. Membrane was probed with 1 µg/mL of Human PD‑1 Monoclonal Antibody (Catalog # MAB1086). PD‑1 band was detected at approximately 33 kDa
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