Human Nrf2 Antibody

Detection of Human Nrf2 by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-), treated (+) or mock treated (m) with 10 µM MG132 (Catalog # 1748) for 4 hours and 10 pmol siRNA. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Nrf2 Monoclonal Antibody (Catalog # MAB3925) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Nrf2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Nrf2 by Western Blot

Confirmation of the IPA-predicted NRF2 activation in cancer cell lines by Western blot. Western blot analysis with NRF2-specific antibody in four different cell extracts per cell line (SiHa, HeLa, C33A, and HCK1T), corresponding to four biological replicates. Twenty μg was loaded. (a) A protein band of 70 kDa corresponding to NRF2 is detected. Immunoblotting for alpha-tubulin (50 kDa) was applied to ensure the comparable loading of proteins in each lane. Fold expression of NRF2 was assessed relative to HCK1T. (b) The mean NRF2 values for SiHa, HeLa, and C33A were 1.4 ± 0.1 (p < 0.05), 1.1 ± 0.2 (p > 0.05), and 2.3 ± 0.3 (p < 0.05) compared to HCK1T, respectively. Representative images of two biological replicates are shown for each cell line. Graphical representation of densitometry analysis of the results (mean ± SD) is also shown (∗p < 0.05, Student's t-test). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28261610), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Rat Nrf2 by Western Blot

Immunoblot analysis of kidney lysates.(A) Blot membranes were incubated with the antibodies against Nrf2, p53 and 8-oxoguanine glycosylase alpha (OGG1 alpha). The mean integrated optical density is related to actin for Nrf2 (B), p53 (C) and mature 8-oxoguanine glycosylase alpha (D) levels. Results are given as a mean ± S.E.M. KD4, KD6, KD8 – groups of Eker rats (Tsc2+/−) treated with HFKD for four, six or eight mo., respectively; ST – Eker rats fed with a standard diet; LE ST – wild-type Long Evans rats treated with a standard diet; LE KD – wild-type Long Evans rats treated with a ketogenic diet similarly to the KD6 group. *P < 0.05, **p < 0.01, ***p < 0.001 as compared to ST unless otherwise stated (horizontal line) for ANOVA with a Fisher post hoc test. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26892894), licensed under a CC-BY license. Not internally tested by R&D Systems.