Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

PE (Excitation = 488 nm, Emission = 575 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 383727

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Nrf2 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Nrf2
Met17-Asn605
Accession # Q16236

Specificity

Detects human Nrf2 in Western blots. In Western blots, no cross-reactivity with mouse and rat Nrf2 and human Nrf1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Nrf2 Antibody (383727) [PE]

Application
Recommended Usage

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Nrf2

Nrf2 is a transcription factor which belongs to the Cap N Collar (CNC-bZIP) subfamily with an implication in adaptive responses to oxidative stress. Nrf2 is a basic leucine zipper transcription factor that can bind to a NF-E2/AP-1 repeat sequence in the promoter of the beta-globin gene and has two types of binding partners, a cytoplasmic repressor (Keap I) and multiple nuclear binding partners. During oxidative stress on a cell, Nrf2 is released from Keap I, possibly via PKC phosphorylation at serine 40, and translocates to the nucleus. In the nucleus, Nrf2 binds to antioxidant responsive element (ARE) and leads to transcriptional activation of downstream genes for GSTs, glutamate-cysteine ligase, HO-1, and thioredoxin. Nrf2 and its downstream proteins have shown to have important functions in protection against oxidative stress or chemical-induced cellular damage in liver and lung, prevention in cancer formation in GI tract, and promotion of the wound-healing process.

Long Name

NF-E2-related Factor 2

Alternate Names

NFE2L2

Gene Symbol

NFE2L2

Additional Nrf2 Products

Product Documents for Nrf2 Antibody (383727) [PE]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Nrf2 Antibody (383727) [PE]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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