Human NKG2D/CD314 Antibody

Name: Human NKG2D/CD314 Antibody
Brand: R&D Systems
SKU: MAB139
Rating: 5 (2 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB139

Citations (82)

(2)

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Clone 149810 was used by HLDA to establish CD designation

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Setting the standard in quality research reagents

Key Product Details

Species Reactivity:	Human
Immunogen:	BaF3 mouse pro-B cell line transfected with human NKG2D/CD314
Applications:	Block/Neutralize, CyTOF-ready, Flow Cytometry, Functional Assay, Western Blot
Label:	Unconjugated
Source:	Monoclonal Mouse IgG₁ Clone # 149810
Specificity:	Detects human NKG2D/CD314 in Western blots.

View Full Technical Details for Human NKG2D/CD314 Antibody

Applications for Human NKG2D/CD314 Antibody

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Human NKG2D/CD314 Fc Chimera (Catalog # 1299-NK) under non-reducing conditions only
Flow Cytometry	0.25 µg/10⁶ cells	See below
Costimulation of T Cells	Rincon-Orozco, B. et al. (2005) J. Immunol. 175:2144.
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Item	Value
Neutralization	Kwong, K.Y. et al. (2008) J. Mol. Biol. 384:1143.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human NKG2D/CD314 Antibody

	Flow Cytometry Detection of NKG2D/CD314 in Human PBMCs gated on CD3- cells by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) gated on CD3- cells were stained with Mouse Anti-Human NCAM-1/CD56 APC-conjugated Monoclonal Antibody (Catalog # FAB2408A) and either (A) Mouse Anti-Human NKG2D/CD314 Monoclonal Antibody (Catalog # MAB139) or (B) Mouse IgG1 Isotype Control (Catalog # MAB002) followed by anti-Mouse IgG PE-conjugated Monoclonal Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
	Flow Cytometry Detection of NKG2D/CD314 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD8 APC-conjugated Monoclonal Antibody (FAB1509A) and either (A) Mouse Anti-Human NKG2D/CD314 Monoclonal Antibody (Catalog # MAB139) or (B) Mouse IgG1 Isotype Control (MAB002) followed by anti-Mouse IgG PE-conjugated Monoclonal Antibody (F0102B). Staining was performed using our Staining Membrane-associated Proteins protocol.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: NKG2D/CD314

NKG2D, also known as CD314, is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C‑type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8⁺ alpha beta T cells, gamma δ T cells, NK cells, and NKT cells. In mouse systems NKG2D also occurs on macrophages. Human ligands for NKG2D include MICA, MICB, and ULBP1, 2, and 3. Expression of NKG2D ligands occurs in epithelial cells, tumor cells and under conditions of stress or infection. NKG2D exists as a disulfide-linked homodimer that delivers an activating signal upon ligand binding. Signaling requires association with an adapter protein. Alternative splicing of the NKG2D mRNA results in isoforms with different cytoplasmic domains that can associate either with DAP12 to deliver a true activating signal or with DAP10 resulting in a costimulatory signal. NKG2D has been implicated in anti-tumor surveillance and the immune response against viral infection.

Item	Value
References	Li, P. et al. (2001) Nature Immunol. 2:443. Steinle, A. et al. (2001) Immunogenetics 53:279. Cosman, D. et al. (2001) Immunity 14:123. Cerwenka, A. and L. Lanier (2001) Immunol. Rev. 181:158. Wu, J. et al. (1999) Science 285:730. Diefenbach, A. et al. (2002) Nature Immunol. 3:1142. Gilfillan, S. et al. (2002) Nature Immunol. 3:1150. Groh, V. et al. (2001) Nature Immunol. 2:255. Cerwenka, A. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11521. Diefenbach, A. et al. (2001) Nature 413:165. NKG2D and its Ligands (2002) www.RnDSystems.com.
Long Name	Natural Killer G2D
Entrez Gene IDs	22914 (Human); 27007 (Mouse); 102120479 (Cynomolgus Monkey)
Alternate Names	CD314, CD314 antigen, D12S2489E, FLJ17759, FLJ75772, KLR, KLRK1, Killer cell lectin-like receptor subfamily K member 1, NK cell receptor D, NKG2-D, NKG2-D type II integral membrane protein, NKG2-D-activating NK receptor, NKG2D, NKG2DDNA segment on chromosome 12 (unique) 2489 expressed sequence, killer cell lectin-like receptor subfamily K, member 1

Citations for Human NKG2D/CD314 Antibody (82)

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Citations are publications that use Bio-Techne products. Selected citations for Human NKG2D/CD314 Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: Neutralization

N Allende-Ve et al. (2022), Metformin sensitizes leukemic cells to cytotoxic lymphocytes by increasing expression of intercellular adhesion molecule-1 (ICAM-1) Scientific Reports, 12(1):1341.
PMID: 35079096
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

HY Wu et al. (2022), Venetoclax enhances NK cell killing sensitivity of AML cells through the NKG2D/NKG2DL activation pathway International immunopharmacology, 104(0):108497.
PMID: 34999394
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

S Belkahla et al. (2022), The metabolism of cells regulates their sensitivity to NK cells depending on p53 status Scientific Reports, 12(1):3234.
PMID: 35217717
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

MC Puzzolo et al. (2022), Immunomodulatory Effects of IFNalpha on T and NK Cells in Chronic Myeloid Leukemia Patients in Deep Molecular Response Preparing for Treatment Discontinuation Journal of Clinical Medicine, 11(19).
PMID: 36233461
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

R Chimienti et al. (2022), Engineering of immune checkpoints B7-H3 and CD155 enhances immune compatibility of MHC-I-/- iPSCs for beta cell replacement Cell Reports, 40(13):111423.
PMID: 36170817
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

X Zhong et al. (2021), The diterpenoid adenanthin upregulates the expression of natural killer group 2D receptor ligands in hepatocellular carcinoma cells Molecular and cellular probes, 59(0):101759.
PMID: 34265372
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

E Seidel et al. (2021), The human cytomegalovirus protein UL147A downregulates the most prevalent MICA allele: MICA*008, to evade NK cell-mediated killing PloS Pathogens, 17(5):e1008807.
PMID: 33939764
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

S Petillo et al. (2021), Immunomodulatory effect of NEDD8-activating enzyme inhibition in Multiple Myeloma: upregulation of NKG2D ligands and sensitization to Natural Killer cell recognition Cell Death Disease, 2021;12(9):836.
PMID: 34482362
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

D Westhoff S et al. (2021), The Epstein-Barr Virus Oncogene EBNA1 Suppresses Natural Killer Cell Responses and Apoptosis Early after Infection of Peripheral B Cells MBio, 0(0):e0224321.
PMID: 34781735
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

S Capellero et al. (2020), Preclinical immunotherapy with Cytokine-Induced Killer lymphocytes against epithelial ovarian cancer Sci Rep, 10(1):6478.
PMID: 32296104
Species: Human
Sample Types: Cell Culture Supernates
Applications: Neutralization

M Diab et al. (2020), Human Metapneumovirus Escapes NK Cell Recognition through the Downregulation of Stress-Induced Ligands for NKG2D Viruses, 12(7).
PMID: 32698530
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

B Müller-Dur et al. (2019), CD56-negative NK cells with impaired effector function expand in CMV and EBV co-infected healthy donors with age Aging (Albany NY), 11(2):724-740.
PMID: 30686790
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

CF Amorim et al. (2019), The Role of NK Cells in the Control of Viral Infection in HTLV-1 Carriers J Immunol Res, 2019(0):6574828.
PMID: 30944834
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

E Oh et al. (2019), Cryopreserved Human Natural Killer Cells Exhibit Potent Antitumor Efficacy against Orthotopic Pancreatic Cancer through Efficient Tumor-Homing and Cytolytic Ability (Running Title: Cryopreserved NK Cells Exhibit Antitumor Effect) Cancers (Basel), 11(7).
PMID: 31324057
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

A Gras Navar et al. (2019), Pretreatment of Glioblastoma with Bortezomib Potentiates Natural Killer Cell Cytotoxicity through TRAIL/DR5 Mediated Apoptosis and Prolongs Animal Survival Cancers (Basel), 11(7).
PMID: 31319548
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

HG Wirsching et al. (2019), Arming oHSV with ULBP3 drives abscopal immunity in lymphocyte-depleted glioblastoma JCI Insight, 4(13).
PMID: 31292299
Species: Human
Sample Types: Whole Cells
Applications: Stimulation

KM Wilton et al. (2019), NKG2D/DAP10 Signaling recruits EVL to the cytotoxic synapse to generate F-actin and promote NK cell cytotoxicity J. Cell. Sci., 0(0).
PMID: 31235500
Species: Human
Sample Types: Whole Cells
Applications: IHC

DP Muñoz et al. (2019), Targetable mechanisms driving immunoevasion of persistent senescent cells link chemotherapy-resistant cancer to aging JCI Insight, 5(0).
PMID: 31184599
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

YS Lee et al. (2018), The combination of ionizing radiation and proteasomal inhibition by bortezomib enhances the expression of NKG2D ligands in multiple myeloma cells J. Radiat. Res., 0(0).
PMID: 29518205
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

YM Dai et al. (2018), EBV transformation induces overexpression of hMSH2/3/6 on B lymphocytes and enhances ??T cell-mediated cytotoxicity via TCR and NKG2D Immunology, 0(0).
PMID: 29512904
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

S López-Cobo et al. (2018), Impaired NK cell recognition of vemurafenib-treated melanoma cells is overcome by simultaneous application of histone deacetylase inhibitors Oncoimmunology, 7(2):e1392426.
PMID: 29308322
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay

O Matalon et al. (2018), Actin retrograde flow controls natural killer cell response by regulating the conformation state of SHP-1 EMBO J., 0(0).
PMID: 29449322
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

J Prévost et al. (2018), Incomplete downregulation of CD4 expression affects HIV-1 Env conformation and ADCC responses J. Virol., 0(0).
PMID: 29669829
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

JRM Van Audena et al. (2017), Interleukin-15 stimulates natural killer cell-mediated killing of both human pancreatic cancer and stellate cells Oncotarget, 0(0).
PMID: 28615524
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

F Lin et al. (2017), Prognostic significance and functional implication of immune activating receptor NKG2D in gastric cancer Biochem. Biophys. Res. Commun., 0(0).
PMID: 28433635
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay

H Jensen et al. (2017), EBI3 regulates the NK cell response to mouse cytomegalovirus infection Proc. Natl. Acad. Sci. U.S.A, 0(0).
PMID: 28143936
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Y Charpak-Am et al. (2017), Human cytomegalovirus escapes immune recognition by NK cells through the downregulation of B7-H6 by the viral genes US18 and US20 Sci Rep, 7(1):8661.
PMID: 28819195
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

S Santaguida et al. (2017), Chromosome Mis-segregation Generates Cell-Cycle-Arrested Cells with Complex Karyotypes that Are Eliminated by the Immune System Dev. Cell, 41(6):638-651.e5.
PMID: 28633018
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Z Djaoud et al. (2017), Two alternate strategies for innate immunity to Epstein-Barr virus: One using NK cells and the other NK cells and ?? T cells J. Exp. Med., 0(0).
PMID: 28468758
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay

X Cai et al. (2017), Control of Tumor Initiation by NKG2D Naturally Expressed on Ovarian Cancer Cells Neoplasia, 19(6):471-482.
PMID: 28499126
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

C Niu et al. (2017), Low-dose bortezomib increases the expression of NKG2D and DNAM-1 ligands and enhances induced NK and ?? T cell-mediated lysis in multiple myeloma Oncotarget, 8(4):5954-5964.
PMID: 27992381
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

(2016), A functional polymorphism in the NKG2D gene modulates NK-cell cytotoxicity and is associated with susceptibility to Human Papilloma Virus-related cancers Sci Rep, 6(0):39231.
PMID: 27995954
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

M Ke et al. (2016), SEP enhanced the antitumor activity of 5-fluorouracil by up-regulating NKG2D/MICA and reversed immune suppression via inhibiting ROS and caspase-3 in mice Oncotarget, 7(31):49509-49526.
PMID: 27385218
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

(2016), NK cell function is markedly impaired in patients with chronic lymphocytic leukaemia but is preserved in patients with small lymphocytic lymphoma Oncotarget, 0(0).
PMID: 27655680
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Frishman-Levy L et al. (2015), Central nervous system acute lymphoblastic leukemia: role of natural killer cells. Blood, 125(22):3420-31.
PMID: 25896649
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Kruse V et al. (2015), Human Induced Pluripotent Stem Cells Are Targets for Allogeneic and Autologous Natural Killer (NK) Cells and Killing Is Partly Mediated by the Activating NK Receptor DNAM-1. PLoS ONE, 10(5):e0125544.
PMID: 25950680
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Giannattasio A et al. (2015), Cytotoxicity and infiltration of human NK cells in in vivo-like tumor spheroids. BMC Cancer, 15(0):351.
PMID: 25933805
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Awad A et al. (2014), Natural killer cells induce eosinophil activation and apoptosis. PLoS ONE, 9(4):e94492.
PMID: 24727794
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Serrano-Pertierra E et al. (2014), L-plastin is involved in NKG2D recruitment into lipid rafts and NKG2D-mediated NK cell migration. J Leukoc Biol, 96(3):437-45.
PMID: 24803550
Species: Human
Sample Types: Whole Cells
Applications: ICC

Molfetta R et al. (2014), c-Cbl regulates MICA- but not ULBP2-induced NKG2D down-modulation in human NK cells. Eur J Immunol, 44(9):2761-70.
PMID: 24846123
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Sangiolo et al. (2014), Cytokine-induced killer cells eradicate bone and soft-tissue sarcomas. Cancer Res, 74(1):119-29.
PMID: 24356422
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Karimi M et al. (2014), A truncated human NKG2D splice isoform negatively regulates NKG2D-mediated function. J Immunol, 193(6):2764-71.
PMID: 25092887
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Celis-Gutierrez J et al. (2014), Dok1 and Dok2 proteins regulate natural killer cell development and function. EMBO J, 33(17):1928-40.
PMID: 24963146
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Lim S et al. (2013), Ex vivo expansion of highly cytotoxic human NK cells by cocultivation with irradiated tumor cells for adoptive immunotherapy. Cancer Res, 73(8):2598-607.
PMID: 23580577
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay

Ham et al. (2013), Dedicator of cytokinesis 8 interacts with talin and Wiskott-Aldrich syndrome protein to regulate NK cell cytotoxicity. J Immunol, 190(7):3661-9.
PMID: 23455509
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Hosomi S et al. (2013), CEACAM1 on activated NK cells inhibits NKG2D-mediated cytolytic function and signaling. Eur J Immunol, 43(9):2473-83.
PMID: 23696226
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Windheim et al. (2013), A unique secreted adenovirus E3 protein binds to the leukocyte common antigen CD45 and modulates leukocyte functions. Proc Natl Acad Sci U S A, 110(50):E4884-93.
PMID: 24218549
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Ho et al. (2013), The road to memory: an early rest for the long journey. J Immunol, 191(11):5603-14.
PMID: 24184558
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Di Carlo E et al. (2013), Mechanisms of the antitumor activity of human Vgamma9Vdelta2 T cells in combination with zoledronic acid in a preclinical model of neuroblastoma. Mol Ther, 21(5):1034-43.
PMID: 23481325
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Leung W et al. (2013), Modulation of NKG2D ligand expression and metastasis in tumors by spironolactone via RXRgamma activation. J Exp Med, 210(12):2675-92.
PMID: 24190430
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Glassner A et al. (2012), NK cells from HCV-infected patients effectively induce apoptosis of activated primary human hepatic stellate cells in a TRAIL-, FasL- and NKG2D-dependent manner. Lab. Invest., 92(7):967-77.
PMID: 22449797
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay

Nielsen N et al. (2012), Cytotoxicity of CD56(bright) NK cells towards autologous activated CD4+ T cells is mediated through NKG2D, LFA-1 and TRAIL and dampened via CD94/NKG2A. PLoS ONE, 7(2):e31959.
PMID: 22384114
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Tsukerman P et al. (2012), MiR-10b Downregulates the Stress-Induced Cell Surface Molecule MICB, a Critical Ligand for Cancer Cell Recognition by Natural Killer Cells. Cancer Res, 72(21):5463-72.
PMID: 22915757
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay

(2012), Wiskott-Aldrich syndrome protein (WASp) and N-WASp are involved in the regulation of NK-cell migration upon NKG2D activation. Eur J Immunol, 42(8):2142-51.
PMID: 22585739
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Andresen L et al. (2012), 2-deoxy D-glucose prevents cell surface expression of NKG2D ligands through inhibition of N-linked glycosylation. J. Immunol., 188(4):1847-55.
PMID: 22227571
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Brown A et al. (2012), Super-resolution imaging of remodeled synaptic actin reveals different synergies between NK cell receptors and integrins. Blood, 120(18):3729-40.
PMID: 22966166
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Heinemann A et al. (2012), Tumor suppressive microRNAs miR-34a/c control cancer cell expression of ULBP2, a stress-induced ligand of the natural killer cell receptor NKG2D. Cancer Res., 72(2):460-71.
PMID: 22102694
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Morandi F et al. (2012), Human TCR gamma delta+ T cells represent a novel target for IL-27 activity. Eur. J. Immunol., 42(6):1547-52.
PMID: 22678908
Species: Human
Sample Types: Whole Cells
Applications: Blocking

Donia M et al. (2012), Analysis of Vdelta1 T cells in clinical grade melanoma-infiltrating lymphocytes. Oncoimmunology, 1(8):1297-1304.
PMID: 23243593
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Inoue T et al. (2011), Potent anti-tumor killing activity of the multifunctional Treg cell line HOZOT against human tumors with diverse origins. Int. J. Oncol., 38(5):1299-306.
PMID: 21373756
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay

Angelini DF et al. (2011), NKG2A inhibits NKG2C effector functions of gammadelta T cells: implications in health and disease. J. Leukoc. Biol., 89(1):75-84.
PMID: 20952657
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Ardolino M et al. (2011), DNAM-1 ligand expression on Ag-stimulated T lymphocytes is mediated by ROS-dependent activation of DNA-damage response: relevance for NK-T cell interaction. Blood, 117(18):4778-86.
PMID: 21406724
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay

Qin H et al. (2011), Natural killer cells from children with type 1 diabetes have defects in NKG2D-dependent function and signaling. Diabetes, 60(3):857-66.
PMID: 21270236
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Cabezon R et al. (2011), Analysis of HLDA9 mAbs on plasmacytoid dendritic cells. Immunol. Lett., 134(2):167-73.
PMID: 20933012
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Maniar A et al. (2010), Human gammadelta T lymphocytes induce robust NK cell-mediated antitumor cytotoxicity through CD137 engagement. Blood, 116(10):1726-33.
PMID: 20519625
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Cho D et al. (2010), Cytotoxicity of activated natural killer cells against pediatric solid tumors. Clin. Cancer Res., 16(15):3901-9.
PMID: 20542985
Species: Human
Sample Types: Whole Cells
Applications: Blocking

Schwinn N et al. (2009), Interferon-gamma down-regulates NKG2D ligand expression and impairs the NKG2D-mediated cytolysis of MHC class I-deficient melanoma by natural killer cells. Int. J. Cancer, 124(7):1594-604.
PMID: 19089914
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay

Purdy AK et al. (2009), SHP-2 expression negatively regulates NK cell function. J. Immunol., 183(11):7234-43.
PMID: 19915046
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Markel G et al. (2009), Natural killer lysis receptor (NKLR)/NKLR-ligand matching as a novel approach for enhancing anti-tumor activity of allogeneic NK cells. PLoS ONE, 4(5):e5597.
PMID: 19440333
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay

Cerboni C et al. (2009), Detuning CD8+ T lymphocytes by down-regulation of the activating receptor NKG2D: role of NKG2D ligands released by activated T cells. Blood, 113(13):2955-64.
PMID: 19124832
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Tang KF et al. (2008), Induction of MHC class I-related chain B (MICB) by 5-aza-2'-deoxycytidine. Biochem. Biophys. Res. Commun., 370(4):578-83.
PMID: 18395517
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Verhoeven DH et al. (2008), NK cells recognize and lyse Ewing sarcoma cells through NKG2D and DNAM-1 receptor dependent pathways. Mol. Immunol., 45(15):3917-25.
PMID: 18657862
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay

Dulphy N et al. (2008), An unusual CD56(bright) CD16(low) NK cell subset dominates the early posttransplant period following HLA-matched hematopoietic stem cell transplantation. J. Immunol., 181(3):2227-37.
PMID: 18641363
Species: Human
Sample Types: Peptide
Applications: Surface Plasmon Resonance

Kwong KY et al. (2008), Generation, affinity maturation, and characterization of a human anti-human NKG2D monoclonal antibody with dual antagonistic and agonistic activity. J. Mol. Biol., 384(5):1143-56.
PMID: 18809410
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay

Zhang H et al. (2007), 4-1BB is superior to CD28 costimulation for generating CD8+ cytotoxic lymphocytes for adoptive immunotherapy. J. Immunol., 179(7):4910-8.
PMID: 17878391
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Shi J et al. (2007), Bortezomib down-regulates the cell-surface expression of HLA class I and enhances natural killer cell-mediated lysis of myeloma. Blood, 111(3):1309-17.
PMID: 17947507
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Kim YJ et al. (2007), 4-1BB regulates NKG2D costimulation in human cord blood CD8+ T cells. Blood, 111(3):1378-86.
PMID: 18024793
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Roda-Navarro P et al. (2006), Transfer of NKG2D and MICB at the cytotoxic NK cell immune synapse correlates with a reduction in NK cell cytotoxic function. Proc. Natl. Acad. Sci. U.S.A., 103(30):11258-63.
PMID: 16849432
Species: Human
Sample Types: Recombinant Protein
Applications: Neutralization

Hanabuchi S et al. (2006), Human plasmacytoid predendritic cells activate NK cells through glucocorticoid-induced tumor necrosis factor receptor-ligand (GITRL). Blood, 107(9):3617-23.
PMID: 16397134
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Brando C et al. (2005), Receptors and lytic mediators regulating anti-tumor activity by the leukemic killer T cell line TALL-104. J. Leukoc. Biol., 78(2):359-71.
PMID: 15937142
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Terme M et al. (2005), BCR/ABL promotes dendritic cell-mediated natural killer cell activation. Cancer Res., 65(14):6409-17.
PMID: 16024645
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Davis Z et al. (0), CD155 on HIV-Infected Cells Is Not Modulated by HIV-1 Vpu and Nef but Synergizes with NKG2D Ligands to Trigger NK Cell Lysis of Autologous Primary HIV-Infected Cells. AIDS Res Hum Retroviruse33(2):93-100.
PMID: 27296670

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Human NKG2D/CD314 Antibody

Name: Anonymous

Application: Flow

Sample Tested: Peripheral blood mononuclear cells (PBMCs)

Species: Human

Verified Customer | Posted 01/24/2021
Human NKG2D/CD314 Antibody

Name: Leslie Priddy

Application: IHC

Sample Tested: Paraffined tumor tissue from HCC xenograft

Species: Human

Verified Customer | Posted 04/13/2018

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Human NKG2D/CD314 Antibody

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Background: NKG2D/CD314

Citations for Human NKG2D/CD314 Antibody (82)

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Human NKG2D/CD314 Antibody

Human NKG2D/CD314 Antibody

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