Detects human MUC-1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) MUC-20, rhMUC-20S, or rhCA125/MUC-16 was observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human MUC-1 Antibody

MUC1 in Human Pancreas Tumor via seqIF™ staining on COMET™

MUC1 was detected in immersion fixed paraffin-embedded sections of human pancreas tumor using Mouse Anti-Human MUC1, Monoclonal Antibody (Catalog # MAB6298) at 4ug/mL at 27 ° Celsius for 2 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.

MUC1 in Human Colon via seqIF™ staining on COMET™

MUC1 was detected in immersion fixed paraffin-embedded sections of human colon using Mouse Anti-Human MUC1, Monoclonal Antibody (Catalog # MAB6298) at 4ug/mL at 27 ° Celsius for 2 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.

MUC1 in Human Breast Tumor via seqIF™ staining on COMET™

MUC1 was detected in immersion fixed paraffin-embedded sections of human breast tumor using Mouse Anti-Human MUC1, Monoclonal Antibody (Catalog # MAB6298) at 4ug/mL at 27 ° Celsius for 2 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.

Detection of Human MUC‑1 by Western Blot.

Western blot shows lysates of human lung tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human MUC-1 Monoclonal Antibody (Catalog # MAB6298) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for MUC-1 at approximately 300 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Detection of MUC-1 in MCF‑7 Human Cell Line by Flow Cytometry.

MCF-7 human breast cancer cell line was stained with Mouse Anti-Human MUC-1 Monoclonal Antibody (Catalog # MAB6298, filled histogram) or isotype control antibody (MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B).

MUC‑1 in Capan‑1 Human Cell Line.

MUC-1 was detected in immersion fixed Capan-1 human pancreatic adenocarcinoma cell line using Mouse Anti-Human MUC-1 Monoclonal Antibody (Catalog # MAB6298) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

MUC‑1 in Human Breast.

MUC-1 was detected in immersion fixed paraffin-embedded sections of human breast using Mouse Anti-Human MUC-1 Monoclonal Antibody (Catalog # MAB6298) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human MUC‑1 by Simple Western^TM.

Simple Western lane view shows lysates of human lung tissue, loaded at 0.2 mg/mL. Specific bands were detected for MUC‑1 at approximately 300-500 kDa (as indicated) using 40 µg/mL of Mouse Anti-Human MUC‑1 Monoclonal Antibody (Catalog # MAB6298). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.

Detection of Human MUC1 by Immunocytochemistry/Immunofluorescence

Co-localization of PAM4 antigen with MUC5AC by immunofluorescence staining(A) Mucin-expressing cell lines were stained with DAPI, hPAM4, and anti-MUC5AC (2-12M1 for Capan-1 and BxPC-3; 2-11M1 for HT-29 and MCF-7), then examined by immunofluorescence microcopy. (B) BxPC-3 and HT-29 cells were stained with DAPI, hPAM4, and alpha-MUC1. PAM4 antigen was shown to co-localize with MUC5AC, not MUC1. Image collected and cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.2760), licensed under a CC-BY license. Not internally tested by R&D Systems.

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Applications for Human MUC-1 Antibody

Application

Recommended Usage

COMET

4 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast tumor, colon and pancreas tumor

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: MCF‑7 human breast cancer cell line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed Capan-1 human pancreatic adenocarcinoma cell line

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast

Simple Western

40 µg/mL
Sample: Human lung tissue

Western Blot

2 µg/mL
Sample: Human lung tissue under non-reducing conditions only

Reviewed Applications

Read 1 review rated 5 using MAB6298 in the following applications:

Immunohistochemistry (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MUC-1

MUC-1 (Mucin-1; also PEM, PEMT, Episialin, tumor-associated mucin, and carcinoma associated mucin) is a 240-450 kDa type I transmembrane glycoprotein. Human MUC-1 is 1255 amino acids (aa) in length and contains a 23 aa signal sequence, a 1135 aa extracellular domain (ECD), a 23 aa transmembrane segment, and a 74 aa cytoplasmic tail. The ECD consists of degenerate tandem repeats and a tandem repeat region, which makes up the major portion of the protein. Potential O-glycosylation sites (serines and threonines) make up more than one-fourth of the amino acids. Splicing variants produce ten isoforms for human MUC-1. Human MUC-1 is 28% aa identical to mouse MUC-1.

Long Name

Mucin 1, Cell Surface-associated

Alternate Names

CD227, Episialin, H23AG, KL-6, Mucin-1, PEM, PEMT

Entrez Gene IDs

4582 (Human)

Gene Symbol

MUC1

UniProt

P15941

Additional MUC-1 Products

Product Documents for Human MUC-1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Human MUC-1 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

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Human MUC-1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6298

Key Product Details

Species Reactivity

Validated:

Cited:

Applications