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Human/Mouse/Rat SOX2 Antibody Best Seller

Bio-Techne includes R&D Systems | Catalog # AF2018

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AF2018
AF2018-SP

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Chromatin Immunoprecipitation (ChIP), Immunocytochemistry, Immunohistochemistry, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Summary for Human/Mouse/Rat SOX2 Antibody

Immunogen

E. coli-derived recombinant human SOX2
Gly135-Met317
Accession # P48431

Specificity

Detects human SOX2 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat SOX2 Antibody

Detection of Human, Mouse, and Rat SOX2 antibody by Western Blot.

Detection of Human, Mouse, and Rat SOX2 by Western Blot.

Western blot shows lysates of D3 mouse embryonic stem cell line, NTera-2 human testicular embryonic carcinoma cell line, F9 mouse teratocarcinoma stem cells, and rat cortical stem cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat SOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2018) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for SOX2 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of SOX2-regulated Genes antibody by Chromatin Immunoprecipitation.

Detection of SOX2-regulated Genes by Chromatin Immunoprecipitation.

BG01V human embryonic stem cells were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. SOX2/DNA complexes were immunoprecipitated using 5 µg Goat Anti-Human/Mouse/Rat SOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2018) or control antibody (AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (MAG999) and DNA was purified using chelating resin solution. Thenanogpromoter was detected by standard PCR.
SOX2 antibody in Mouse Cortical Stem Cells by Immunocytochemistry (ICC).

SOX2 in Mouse Cortical Stem Cells.

SOX2 was detected in immersion fixed undifferentiated mouse cortical stem cells using Goat Anti-Human/Mouse/Rat SOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2018) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; (NL001) and counterstained with DAPI (blue). Nestin was also detected in stem cells using Mouse Anti-Mouse/Rat Nestin Monoclonal Antibody (MAB2736) and co-stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; NL009). Specific staining of SOX2 was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Applications for Human/Mouse/Rat SOX2 Antibody

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

5 µg/5 x 106 cells
Sample: BG01V human embryonic stem cells chromatin, for detection see our available Human Nanog Primer Pair (Catalog # RDP-320-025)

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed NTera-2 human testicular embryonic carcinoma cell line, BG01V human embryonic stem cells cultured on irradiated mouse embryonic fibroblasts, undifferentiated mouse cortical stem cells, undifferentiated rat cortical stem cells, ADLF1 and FAB2 induced pluripotent stem cell lines, and HepG2 human hepatocellular carcinoma cells

Immunohistochemistry

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of glioblastoma

Simple Western

5 µg/mL
Sample: BG01V human embryonic stem cells and NTera‑2 human testicular embryonic carcinoma cell line

Western Blot

1 µg/mL
Sample: D3 mouse embryonic stem cell line, NTera‑2 human testicular embryonic carcinoma cell line, F9 mouse teratocarcinoma stem cells, and rat cortical stem cells
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 13 reviews rated 3.8 using AF2018 in the following applications:

Published Applications

Read 351 publications using AF2018 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SOX2

SOX2 belongs to the SOX (SRY-like HMG box) family of transcription factors with diverse roles in development. SOX2 functions in specifying the first three lineages present at implantation and in regulating proliferation and differentiation in the developing peripheral nervous system (1-5).

References

  1. Graham, V. et al. (2003) Neuron 39:749.
  2. Avilion, A.A. et al. (2003) Genes Dev. 17:126.
  3. Kishi, M. et al. (2000) Development 127:791.
  4. Yuan, H. et al. (1995) Genes Dev. 9:2635.
  5. Uwanogho, D. et al. (1995) Mech. Dev. 49:23.
  6. Stevanovic, M. (2003) Mol. Biol. Rep. 30:127.

Long Name

Transcription Factor SOX2

Alternate Names

ANOP3, MCOPS3, MGC2413, SRY (sex determining region Y)-box 2, SRY-related HMG-box gene 2, transcription factor SOX2, transcription factor SOX-2

Entrez Gene IDs

6657 (Human)

Gene Symbol

SOX2

UniProt

Additional SOX2 Products

Product Documents for Human/Mouse/Rat SOX2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse/Rat SOX2 Antibody

For research use only

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