Human SOX2 Biotinylated Antibody

Name: Human SOX2 Biotinylated Antibody
Brand: R&D Systems
SKU: BAF2018
Price: 494 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # BAF2018

Citations (2)

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Species Reactivity:	Human
Immunogen:	E. coli-derived recombinant human SOX2 Gly135-Met317 Accession # P48431
Applications:	Immunocytochemistry/Immunofluorescence, Western Blot
Label:	Biotin
Source:	Polyclonal Goat IgG
Specificity:	Detects SOX2 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) SOX17 is observed.

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Applications for Human SOX2 Biotinylated Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human SOX2
Immunocytochemistry	5-15 µg/mL	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human SOX2 Biotinylated Antibody

	Immunocytochemistry SOX2 in Human iPSK3 cells. SOX2 was detected in immersion fixed human plasmid-derived induced pluripotent stem cells (iPSK3) using Goat Anti-Human SOX2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2018) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SOX2

SOX2 belongs to the SOX (SRY-like HMG box) family of transcription factors with diverse roles in development. SOX2 functions in specifying the first three lineages present at implantation and in regulating proliferation and differentiation in the developing peripheral nervous system (1-6).

Item	Value
References	Graham, V. et al. (2003) Neuron 39:749. Avilion, A.A. et al. (2003) Genes Dev. 17:126. Kishi, M. et al. (2000) Development 127:791. Yuan, H. et al. (1995) Genes Dev. 9:2635. Uwanogho, D. et al. (1995) Mech. Dev. 49:23. Stevanovic, M. (2003) Mol. Biol. Rep. 30:127.
Long Name	Transcription Factor SOX2
Entrez Gene IDs	6657 (Human)
Alternate Names	ANOP3, MCOPS3, MGC2413, SOX2, SRY (sex determining region Y)-box 2, SRY-related HMG-box gene 2, transcription factor SOX-2, transcription factor SOX2

Citations for Human SOX2 Biotinylated Antibody (2)

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Citations are publications that use Bio-Techne products. Selected citations for Human SOX2 Biotinylated Antibody include:

Species: Human
Sample Types: Whole Tissue
Applications: IHC

N Ohtsu et al. (2016), Eva1 Maintains the Stem-like Character of Glioblastoma-Initiating Cells by Activating the Noncanonical NF-?B Signaling Pathway Cancer Res., 76(1):171-81.
PMID: 26677976
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Auffinger B et al. (2014), Conversion of differentiated cancer cells into cancer stem-like cells in a glioblastoma model after primary chemotherapy. Cell Death Differ, 21(7):1119-31.
PMID: 24608791

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Human SOX2 Biotinylated Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Human SOX2 Biotinylated Antibody

Scientific Data Examples for Human SOX2 Biotinylated Antibody

Immunocytochemistry

Formulation, Preparation and Storage

Reconstitution Calculator

Background: SOX2

Citations for Human SOX2 Biotinylated Antibody (2)

Product Documents for Human SOX2 Biotinylated Antibody

Product Datasheets

COA

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