Human/Mouse/Rat GDF‑8/Myostatin Antibody
Catalog # AF788 | R&D Systems, Inc. a Bio-Techne Brand
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Product Summary for Human/Mouse/Rat GDF‑8/Myostatin Antibody
Immunogen
Asp268-Ser376
Accession # O08689
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human/Mouse/Rat GDF‑8/Myostatin Antibody
Detection of Recombinant Human, Mouse, and Rat GDF-8/Myostatin by Western Blot.
Western blot shows 25 ng of Recombinant Human/Mouse/Rat GDF-8/Myostatin (788-G8), Recombinant Human/Mouse/Rat GDF-11/BMP-11 (Catalog # 1958-GD), Recombinant Human BMP-6 (507-BP), and Recombinant Mouse BMP-6 (Catalog # 6325-BM). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse/Rat GDF-8/Myostatin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF788) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for GDF-8/Myostatin at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.GDF‑8/Myostatin in Mouse Embryo.
GDF-8/Myostatin was detected in immersion fixed frozen sections of mouse embryo (10 d.p.c., section through neural tube) using Goat Anti-Human/Mouse/Rat GDF-8/Myostatin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF788) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Hemoglobin Expression Induced by GDF‑8/Myostatin and Neutralization by Mouse GDF‑8/Myostatin Antibody.
Recombinant Mouse GDF-8/Myostatin (Catalog # 788-G8) increases hemoglobin expression in the K562 human chronic myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the psuedoperoxidase assay. Hemoglobin expression elicited by Recombinant Mouse GDF-8/Myostatin (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Mouse/Rat GDF-8/Myostatin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF788). The ND50 is typically 0.6-3 µg/mL.Applications for Human/Mouse/Rat GDF‑8/Myostatin Antibody
Immunohistochemistry
Sample: Perfusion fixed frozen sections of mouse embryo (10 d.p.c., section through neural tube)
Western Blot
Sample: Recombinant Human/Mouse/Rat GDF‑8/Myostatin (Catalog # 788-G8)
Neutralization
Reviewed Applications
Read 3 reviews rated 4.3 using AF788 in the following applications:
Formulation, Preparation and Storage
Purification
Endotoxin Level
Reconstitution
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GDF-8/Myostatin
References
- Storm, E.E. et al. (1994) Nature 368:639.
- Sharma, M. et al. (1999) J. Cell Physiol. 180:1.
- McPherron, A.C. et al. (1997) Nature 387:83.
- Lee, S.J. et al. (2001) Proc. Natl. Acad. Sci. USA 98:9306.
- Kim, H.S. et al. (2001) Biochem. Biophys. Res. Commun. 281:902.
Long Name
Alternate Names
Gene Symbol
UniProt
Product Documents for Human/Mouse/Rat GDF‑8/Myostatin Antibody
Product Specific Notices for Human/Mouse/Rat GDF‑8/Myostatin Antibody
For research use only
Citations for Human/Mouse/Rat GDF‑8/Myostatin Antibody (10)
Citations are publications that use Bio-Techne products. Selected citations for Human/Mouse/Rat GDF‑8/Myostatin Antibody include:
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Species: Rat
Sample Types: Tissue Homogenates
Applications: Western BlotC Li et al. (2022-09-02), The relationship between myodural bridges, hyperplasia of the suboccipital musculature, and intracranial pressure PLoS ONE, 2022-017(9):e0273193.
PMID: 36054096 -
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western BlotH Muramatsu et al. (2021-01-25), Novel myostatin-specific antibody enhances muscle strength in muscle disease models Scientific Reports, 2021-011(1):2160.
PMID: 33495503 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotQ Jin et al. (2019-05-27), A GDF11/myostatin inhibitor, GDF11 propeptide-Fc, increases skeletal muscle mass and improves muscle strength in dystrophic mdx mice Skelet Muscle, 2019-09(1):16.
PMID: 31133057 -
Species: Mouse
Sample Types: Whole Cells
Applications: NeutralizationC Wallner et al. (2017-08-29), Inhibition of GDF8 (Myostatin) accelerates bone regeneration in diabetes mellitus type 2 Sci Rep, 2017-07(1):9878.
PMID: 28852138 -
Species: Rabbit
Sample Types: Tissue Homogenates
Applications: Western BlotRD Little et al. (2017-07-24), Compensatory anabolic signaling in the sarcopenia of experimental chronic arthritis Sci Rep, 2017-07(1):6311.
PMID: 28740214 -
Species: Human
Sample Types: Tissue Homogenates
Applications: Western BlotJ Ishida et al. (2017-04-04), Myostatin signaling is up-regulated in female patients with advanced heart failure Int. J. Cardiol., 2017-00(0).
PMID: 28465115 -
Species: Human
Sample Types: Protein
Applications: Western BlotO Miroshnych et al. (2016-12-27), The Use of Platelet-Rich and Platelet-Poor Plasma to Enhance Differentiation of Skeletal Myoblasts: Implications for the Use of Autologous Blood Products for Muscle Regeneration Am J Sports Med, 2016-145(4):945-953.
PMID: 28027451 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr(2016-11-22), A single heterochronic blood exchange reveals rapid inhibition of multiple tissues by old blood Nat Commun, 2016-17(0):13363.
PMID: 27874859 -
Species: Rat
Sample Types: Whole Cells
Applications: ICCYamada M et al. (2009-12-09), High concentrations of HGF inhibit skeletal muscle satellite cell proliferation in vitro by inducing expression of myostatin: a possible mechanism for reestablishing satellite cell quiescence in vivo. Am. J. Physiol., Cell Physiol., 2009-1298(3):C465-76.
PMID: 20007454 -
Species: Human
Sample Types: Tissue Homogenates
Applications: Western BlotHittel DS et al. (2008-10-03), Increased secretion and expression of myostatin in skeletal muscle from extremely obese women. Diabetes, 2008-158(1):30-8.
PMID: 18835929
There are no citations that match your criteria.
Customer Reviews for Human/Mouse/Rat GDF‑8/Myostatin Antibody (3)
Customer Images
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Application: Simple WesternSample Tested: Human Skeletal Muscle LysateSpecies: HumanVerified Customer | Posted 06/23/2015
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Application: Western BlotSample Tested: See PMID 23829672Species: HumanVerified Customer | Posted 01/09/2015
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Application: Western BlotSample Tested: See PMID 23297411Species: MouseVerified Customer | Posted 01/09/2015
There are no reviews that match your criteria.
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