Key Product Details
Product Summary for Human/Mouse/Rat GDF‑8/Myostatin Antibody
Accession # O08689
Scientific Data Images for Human/Mouse/Rat GDF‑8/Myostatin Antibody
Detection of Recombinant Human, Mouse, and Rat GDF-8/Myostatin by Western Blot.Western blot shows 25 ng of Recombinant Human/Mouse/Rat GDF-8/Myostatin (788-G8), Recombinant Human/Mouse/Rat GDF-11/BMP-11 (Catalog # 1958-GD), Recombinant Human BMP-6 (507-BP), and Recombinant Mouse BMP-6 (Catalog # 6325-BM). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse/Rat GDF-8/Myostatin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF788) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for GDF-8/Myostatin at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
GDF‑8/Myostatin in Mouse Embryo.GDF-8/Myostatin was detected in immersion fixed frozen sections of mouse embryo (10 d.p.c., section through neural tube) using Goat Anti-Human/Mouse/Rat GDF-8/Myostatin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF788) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Hemoglobin Expression Induced by GDF‑8/Myostatin and Neutralization by Mouse GDF‑8/Myostatin Antibody.Recombinant Mouse GDF-8/Myostatin (Catalog # 788-G8) increases hemoglobin expression in the K562 human chronic myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the psuedoperoxidase assay. Hemoglobin expression elicited by Recombinant Mouse GDF-8/Myostatin (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Mouse/Rat GDF-8/Myostatin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF788). The ND50 is typically 0.6-3 µg/mL.
Applications for Human/Mouse/Rat GDF‑8/Myostatin Antibody
Sample: Perfusion fixed frozen sections of mouse embryo (10 d.p.c., section through neural tube)
Sample: Recombinant Human/Mouse/Rat GDF‑8/Myostatin (Catalog # 788-G8)
Read 3 reviews rated 4.3 using AF788 in the following applications:
Formulation, Preparation and Storage
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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- Lee, S.J. et al. (2001) Proc. Natl. Acad. Sci. USA 98:9306.
- Kim, H.S. et al. (2001) Biochem. Biophys. Res. Commun. 281:902.
Product Specific Notices for Human/Mouse/Rat GDF‑8/Myostatin Antibody
For research use only