Human/Mouse/Rat GDF‑8/Myostatin Antibody

Name: Human/Mouse/Rat GDF‑8/Myostatin Antibody
Brand: R&D Systems
SKU: AF788
Rating: 4 (3 reviews)

Catalog # AF788 | R&D Systems, Inc. a Bio-Techne Brand

(3)

Citations (10)

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Product Summary
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Neutralization, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Summary for Human/Mouse/Rat GDF‑8/Myostatin Antibody

Immunogen

E. coli-derived recombinant mouse GDF-8/Myostatin
Asp268-Ser376
Accession # O08689

Specificity

Detects human, mouse, and rat GDF-8/Myostatin in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat GDF‑8/Myostatin Antibody

Detection of Recombinant Human, Mouse, and Rat GDF-8/Myostatin by Western Blot.

Western blot shows 25 ng of Recombinant Human/Mouse/Rat GDF-8/Myostatin (788-G8), Recombinant Human/Mouse/Rat GDF-11/BMP-11 (Catalog # 1958-GD), Recombinant Human BMP-6 (507-BP), and Recombinant Mouse BMP-6 (Catalog # 6325-BM). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse/Rat GDF-8/Myostatin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF788) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for GDF-8/Myostatin at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

GDF‑8/Myostatin in Mouse Embryo.

GDF-8/Myostatin was detected in immersion fixed frozen sections of mouse embryo (10 d.p.c., section through neural tube) using Goat Anti-Human/Mouse/Rat GDF-8/Myostatin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF788) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Hemoglobin Expression Induced by GDF‑8/Myostatin and Neutralization by Mouse GDF‑8/Myostatin Antibody.

Recombinant Mouse GDF-8/Myostatin (Catalog # 788-G8) increases hemoglobin expression in the K562 human chronic myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the psuedoperoxidase assay. Hemoglobin expression elicited by Recombinant Mouse GDF-8/Myostatin (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Mouse/Rat GDF-8/Myostatin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF788). The ND₅₀ is typically 0.6-3 µg/mL.

Applications for Human/Mouse/Rat GDF‑8/Myostatin Antibody

Application

Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse embryo (10 d.p.c., section through neural tube)

Western Blot

0.1 µg/mL
Sample: Recombinant Human/Mouse/Rat GDF‑8/Myostatin (Catalog # 788-G8)

Neutralization

Measured by its ability to neutralize GDF‑8/Myostatin-induced hemoglobin expression in the K562 human chronic myelogenous leukemia cell line. The Neutralization Dose (ND₅₀) is typically 0.6-3 µg/mL in the presence of 30 ng/mL Recombinant Mouse GDF‑8/Myostatin.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 3 reviews rated 4.3 using AF788 in the following applications:

Simple Western (1 Review)
Western Blot (2 Reviews)

Formulation, Preparation and Storage

Purification

Antigen Affinity-purified

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GDF-8/Myostatin

Growth Differentiation Factor 8 (GDF-8), also known as myostatin, is a member of the TGF-beta superfamily that is expressed specifically in developing and adult skeletal muscle. GDF-8 cDNA encodes a 376 amino acid (aa) prepropeptide with a 24 aa residue signal peptide, a 223 aa residue amino-terminal propeptide, and a 109 aa residue carboxy-terminal mature protein. Mature GDF-8 contains the canonical 7-cysteine motif common to other TGF-beta superfamily members. Similar to the TGF-beta s, activins and BMP-11, GDF-8 also contains one extra pair of cysteine residues that is not found in other family members. The bioactive form of GDF-8 is a homodimer with an apparent molecular weight of approximately 25 kDa. GDF-8 is highly conserved across species. At the amino acid sequence level, mature human, mouse, rat and cow GDF-8 are 100% identical. Within the TGF-beta superfamily, GDF-8 is most closely related to BMP-11, a mammalian protein that acts as a dorsal mesoderm and neural inducer in Xenopus explants. The two proteins share 90% amino acid sequence identity within their mature chain. A targeted disruption of GDF-8 in mouse results in large mice with a widespread increase in skeletal muscle mass, indicating that GDF-8 is a negative regulator of skeletal muscle growth. A mutation in the bovine GDF-8 gene has been shown to be responsible for the double-muscled phenotype in cattle breeds such as Belgian Blue cattle that is characterized by an increase in muscle mass. GDF-8 has also been shown to inhibit preadipocyte differentiation to adipocytes. Mature GDF-8 binds to activin type II receptors and the binding is antagonized by the activin-binding protein, follistatin. R&D Systems recombinant GDF-8 preparations have been shown to act similarly to Activin A in both the Xenopus animal cap and the K562 assays.

References

Storm, E.E. et al. (1994) Nature 368:639.
Sharma, M. et al. (1999) J. Cell Physiol. 180:1.
McPherron, A.C. et al. (1997) Nature 387:83.
Lee, S.J. et al. (2001) Proc. Natl. Acad. Sci. USA 98:9306.
Kim, H.S. et al. (2001) Biochem. Biophys. Res. Commun. 281:902.

Long Name

Growth Differentiation Factor 8

Alternate Names

GDF8, MSLHP, MSTN, Myostatin

Entrez Gene IDs

2660 (Human); 17700 (Mouse)

Gene Symbol

MSTN

UniProt

O08689

Product Documents for Human/Mouse/Rat GDF‑8/Myostatin Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human/Mouse/Rat GDF‑8/Myostatin Antibody

For research use only

Citations
Reviews

Citations for Human/Mouse/Rat GDF‑8/Myostatin Antibody (10)

Showing 1 - 10 of 10 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human/Mouse/Rat GDF‑8/Myostatin Antibody include:

Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot

C Li et al. (2022-09-02), The relationship between myodural bridges, hyperplasia of the suboccipital musculature, and intracranial pressure PLoS ONE, 2022-017(9):e0273193.
PMID: 36054096
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

H Muramatsu et al. (2021-01-25), Novel myostatin-specific antibody enhances muscle strength in muscle disease models Scientific Reports, 2021-011(1):2160.
PMID: 33495503
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Q Jin et al. (2019-05-27), A GDF11/myostatin inhibitor, GDF11 propeptide-Fc, increases skeletal muscle mass and improves muscle strength in dystrophic mdx mice Skelet Muscle, 2019-09(1):16.
PMID: 31133057
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization

C Wallner et al. (2017-08-29), Inhibition of GDF8 (Myostatin) accelerates bone regeneration in diabetes mellitus type 2 Sci Rep, 2017-07(1):9878.
PMID: 28852138
Species: Rabbit
Sample Types: Tissue Homogenates
Applications: Western Blot

RD Little et al. (2017-07-24), Compensatory anabolic signaling in the sarcopenia of experimental chronic arthritis Sci Rep, 2017-07(1):6311.
PMID: 28740214
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot

J Ishida et al. (2017-04-04), Myostatin signaling is up-regulated in female patients with advanced heart failure Int. J. Cardiol., 2017-00(0).
PMID: 28465115
Species: Human
Sample Types: Protein
Applications: Western Blot

O Miroshnych et al. (2016-12-27), The Use of Platelet-Rich and Platelet-Poor Plasma to Enhance Differentiation of Skeletal Myoblasts: Implications for the Use of Autologous Blood Products for Muscle Regeneration Am J Sports Med, 2016-145(4):945-953.
PMID: 28027451
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

(2016-11-22), A single heterochronic blood exchange reveals rapid inhibition of multiple tissues by old blood Nat Commun, 2016-17(0):13363.
PMID: 27874859
Species: Rat
Sample Types: Whole Cells
Applications: ICC

Yamada M et al. (2009-12-09), High concentrations of HGF inhibit skeletal muscle satellite cell proliferation in vitro by inducing expression of myostatin: a possible mechanism for reestablishing satellite cell quiescence in vivo. Am. J. Physiol., Cell Physiol., 2009-1298(3):C465-76.
PMID: 20007454
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot

Hittel DS et al. (2008-10-03), Increased secretion and expression of myostatin in skeletal muscle from extremely obese women. Diabetes, 2008-158(1):30-8.
PMID: 18835929

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