Is Recombinant Human/Mouse/Rat GDF-8/Myostatin Protein (Catalog # 788-G8) the mature form of GDF8?

Name: Recombinant Human/Mouse/Rat GDF-8/Myostatin Protein
Brand: R&D Systems
SKU: 788-G8
Rating: 5 (2 reviews)

The mature form of GDF8 consists of a disulfide-linked homodimer. Catalog 788-G8 consists of Asp268-Ser376. There are multiple disulfide bonds within this sequence, which form a disulfide-linked heterodimer with another Asp268-Ser376 molecule.

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Catalog # 788-G8 | R&D Systems, Inc. a Bio-Techne Brand

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Citations (40)

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Key Product Details

Source:	NS0
Accession #:	O08689
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human/Mouse/Rat GDF-8/Myostatin Protein

Product Specifications for Recombinant Human/Mouse/Rat GDF-8/Myostatin Protein

Source	Mouse myeloma cell line, NS0-derived GDF-8/Myostatin protein Asp268-Ser376
Purity	>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Asp268
Predicted Molecular Mass	12.4 kDa
SDS-PAGE	12 kDa, reducing conditions 24 kDa, non-reducing conditions
Activity	Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cells. Schwall, R.H. et al. (1991) Method Enzymol. 198:340. The ED₅₀ for this effect is 2-10 ng/mL.

Scientific Data Examples for Recombinant Human/Mouse/Rat GDF-8/Myostatin Protein

	Recombinant Human/Mouse/Rat GDF-8/Myostatin Protein Bioactivity Recombinant Human/Mouse/Rat GDF-8/ Myostatin (Catalog # 788-G8) induces hemoglobin expression in the K562 human chronic myelogenous leukemia cell line. The ED₅₀ for this effect is 2-10 ng/mL.
	Recombinant Human/Mouse/Rat GDF-8/Myostatin Protein SDS-PAGE 1 μg/lane of Recombinant Human/Mouse/Rat GDF-8/Myostatin was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing the main bands at 12 kDa and 24 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

788-G8

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. *1 mg pack size (01M) is supplied as a 0.2 µm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

788-G8/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution	Reconstitute at 100 μg/mL in sterile 4 mM HCl.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GDF-8/Myostatin

Growth Differentiation Factor 8 (GDF-8), also known as myostatin, is a member of the TGF-beta superfamily that is expressed specifically in developing and adult skeletal muscle. GDF-8 cDNA encodes a 376 amino acid (aa) prepropeptide with a 24 aa residue signal peptide, a 223 aa residue amino-terminal propeptide, and a 109 aa residue carboxy-terminal mature protein. Mature GDF-8 contains the canonical 7-cysteine motif common to other TGF-beta superfamily members. Similar to the TGF‑ beta s, activins and BMP-11, GDF-8 also contains one extra pair of cysteine residues that is not found in other family members. The bioactive form of GDF-8 is a homodimer with an apparent molecular weight of approximately 25 kDa. GDF-8 is highly conserved across species. At the amino acid sequence level, mature human, mouse, rat and cow GDF-8 are 100% identical. Within the TGF-beta superfamily, GDF-8 is most closely related to BMP-11, a mammalian protein that acts as a dorsal mesoderm and neural inducer in Xenopus explants. The two proteins share 90% amino acid sequence identity within their mature chain. A targeted disruption of GDF-8 in mouse results in large mice with a widespread increase in skeletal muscle mass, indicating that GDF-8 is a negative regulator of skeletal muscle growth. A mutation in the bovine GDF-8 gene has been shown to be responsible for the double-muscled phenotype in cattle breeds such as Belgian Blue cattle that is characterized by an increase in muscle mass. GDF-8 has also been shown to inhibit preadipocyte differentiation to adipocytes. Mature GDF-8 binds to activin type II receptors and the binding is antagonized by the activin-binding protein, follistatin. R&D Systems recombinant GDF-8 preparations have been shown to act similarly to Activin A in both the Xenopus animal cap and the K562 assays.

Item	Value
References	Storm, E.E. et al. (1994) Nature 368:639. Sharma, M. et al. (1999) J. Cell Physiol. 180:1. McPherron, A.C. et al. (1997) Nature 387:83. Lee, S.J. et al. (2001) Proc. Natl. Acad. Sci. USA 98:9306. Kim, H.S. et al. (2001) Biochem. Biophys. Res. Commun. 281:902.
Long Name	Growth Differentiation Factor 8
Entrez Gene IDs	2660 (Human); 17700 (Mouse)
Alternate Names	GDF-8, GDF8, GDF8growth differentiation factor 8, MSLHP, MSTN, Myostatin, growth/differentiation factor 8

Citations for Recombinant Human/Mouse/Rat GDF-8/Myostatin Protein (40)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human/Mouse/Rat GDF-8/Myostatin Protein include:

Species: Human
Sample Types: Transfected Whole Cells
Applications: Bioassay

R Mishra et al. (2022-10-06), Maternal immunization against myostatin suppresses post-hatch chicken growth PLoS ONE, 2022-117(10):e0275753.
PMID: 36201511
Species: Mouse
Sample Types: Serum
Applications: ELISA Capture

DK Sung et al. (2022-08-13), A New Method of Myostatin Inhibition in Mice via Oral Administration of Lactobacillus casei Expressing Modified Myostatin Protein, BLS-M22 International Journal of Molecular Sciences, 2022-023(16).
PMID: 36012334
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

A Hagg et al. (2020-11-04), TMEPAI/PMEPA1 Is a Positive Regulator of Skeletal Muscle Mass Front Physiol, 2020-111(0):560225.
PMID: 33250771
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

YC Chang et al. (2020-07-22), The green tea polyphenol epigallocatechin-3-gallate attenuates age-associated muscle loss via regulation of miR-486-5p and myostatin Arch. Biochem. Biophys., 2020-0692(0):108511.
PMID: 32710883
Species: Mouse
Sample Types: In Vivo, Whole Cells
Applications: Cell Culture, In Vivo

EAC Goossens et al. (2020-05-15), Myostatin Inhibits Vascular Smooth Muscle Cell Proliferation and Local 14q32 microRNA Expression, But Not Systemic Inflammation or Restenosis Int J Mol Sci, 2020-021(10).
PMID: 32429150
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

M Kodaka et al. (2020-04-08), Characterization of a novel compound that promotes myogenesis viaï¿½Akt and transcriptional co-activator with PDZ-binding motif (TAZ) in mouse C2C12 cells PLoS ONE, 2020-015(4):e0231265.
PMID: 32267872
Species: Human
Sample Types: Cell Culture Supernates

KB Dagbay et al. (2020-02-19), Structural basis of specific inhibition of extracellular activation of pro- or latent myostatin by the monoclonal antibody SRK-015 J. Biol. Chem., 2020-0295(16):5404-5418.
PMID: 32075906
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay, Cell Culture

XH Liu et al. (2019-08-01), AdipoRon prevents myostatin-induced upregulation of fatty acid synthesis and downregulation of insulin activity in a mouse hepatocyte line Physiol Rep, 2019-07(13):e14152.
PMID: 31250564
Species: Human
Sample Types: Cell Lysates
Applications: Bioassay

Q Jin et al. (2019-05-27), A GDF11/myostatin inhibitor, GDF11 propeptide-Fc, increases skeletal muscle mass and improves muscle strength in dystrophic mdx mice Skelet Muscle, 2019-09(1):16.
PMID: 31133057
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

JH Kim et al. (2019-04-18), Identification of the minimum region of flatfish myostatin propeptide (Pep45-65) for myostatin inhibition and its potential to enhance muscle growth and performance in animals PLoS ONE, 2019-014(4):e0215298.
PMID: 30998775
Species: Porcine
Sample Types: Whole Tissue
Applications: Bioassay

JD Yoon et al. (2019-02-13), GDF8 enhances SOX2 expression and blastocyst total cell number in porcine IVF embryo development Theriogenology, 2019-0129(0):70-76.
PMID: 30825707
Species: Mouse
Sample Types: Serum

A Iskenderia et al. (2018-10-27), Myostatin and activin blockade by engineered follistatin results in hypertrophy and improves dystrophic pathology in mdx mouse more than myostatin blockade alone Skelet Muscle, 2018-18(1):34.
PMID: 30368252
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

KM Estrellas et al. (2018-08-23), Biological scaffold-mediated delivery of myostatin inhibitor promotes a regenerative immune response in an animal model of Duchenne muscular dystrophy J. Biol. Chem., 2018-00(0).
PMID: 30139748
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

SJ Lessard et al. (2018-08-02), JNK regulates muscle remodeling via myostatin/SMAD inhibition Nat Commun, 2018-09(1):3030.
PMID: 30072727
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

L Formicola et al. (2018-05-24), Inhibition of the Activin Receptor Type-2B Pathway Restores Regenerative Capacity in Satellite Cell-Depleted Skeletal Muscle Front Physiol, 2018-09(0):515.
PMID: 29881353
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

LF Wu et al. (2017-12-16), Relative abundance of mature myostatin rather than total myostatin is negatively associated with bone mineral density in Chinese J. Cell. Mol. Med., 2017-10(0).
PMID: 29247983
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

SA Samant et al. (2017-09-19), The histone deacetylase SIRT6 blocks myostatin expression and development of muscle atrophy Sci Rep, 2017-07(1):11877.
PMID: 28928419
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

JL Chen et al. (2017-06-12), Specific targeting of TGF-? family ligands demonstrates distinct roles in the regulation of muscle mass in health and disease Proc. Natl. Acad. Sci. U.S.A., 2017-00(0).
PMID: 28607086
Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance

E Latres et al. (2017-04-28), Activin A more prominently regulates muscle mass in primates than does GDF8 Nat Commun, 2017-08(0):15153.
PMID: 28452368
Species: Rat
Sample Types: Serum
Applications: Bioassay

Y Zhao et al. (2017-04-26), A multiplexed immunocapture liquid chromatography tandem mass spectrometry assay for the simultaneous measurement of myostatin and GDF-11 in rat serum using an automated sample preparation platform Anal. Chim. Acta, 2017-0979(0):36-44.
PMID: 28599707
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

SB Lee et al. (2017-04-03), Maltose binding protein-fusion enhances the bioactivity of truncated forms of pig myostatin propeptide produced in E. coli PLoS ONE, 2017-012(4):e0174956.
PMID: 28369115
Species: Human, Mouse
Sample Types: Whole Cells
Applications: Bioassay

DW Hammers et al. (2017-04-01), Supraphysiological levels of GDF11 induce striated muscle atrophy EMBO Mol Med, 2017-00(0).
PMID: 28270449
Species: Human, Mouse
Sample Types: In Vivo, Whole Cells
Applications: Bioassay, In Vivo

RG Walker et al. (2017-03-03), Structural basis for potency differences between GDF8 and GDF11 BMC Biol, 2017-015(1):19.
PMID: 28257634
Applications: Control

Tamas Kocsis (2016-12-13), Myostatin propeptide mutation of the hypermuscular Compact mice decreases the formation of myostatin and improves insulin sensitivity Am. J. Physiol. Endocrinol. Metab, 2016-10(0):ajpendo.00216.
PMID: 27965203
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Latres E et al. (2015-10-09), Myostatin blockade with a fully human monoclonal antibody induces muscle hypertrophy and reverses muscle atrophy in young and aged mice. Skelet Muscle, 2015-15(0):34.
PMID: 26457176
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Seto D et al. (2015-06-19), A Key Role for Leukemia Inhibitory Factor in C26 Cancer Cachexia. J Biol Chem, 2015-0290(32):19976-86.
PMID: 26092726
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Horbelt D et al. (2014-11-03), Small molecules dorsomorphin and LDN-193189 inhibit myostatin/GDF8 signaling and promote functional myoblast differentiation. J Biol Chem, 2014-1290(6):3390-404.
PMID: 25368322
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Relizani K et al. (2014-05-27), Blockade of ActRIIB signaling triggers muscle fatigability and metabolic myopathy. Mol Ther, 2014-022(8):1423-33.
PMID: 24861054
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Biesemann N et al. (2014-05-07), Myostatin regulates energy homeostasis in the heart and prevents heart failure. Circ Res, 2014-0115(2):296-310.
PMID: 24807786
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Yang et al. (2014-02-18), Screening with a novel cell-based assay for TAZ activators identifies a compound that enhances myogenesis in C2C12 cells and facilitates muscle repair in a muscle injury model. Mol Cell Biol, 2014-034(9):1607-21.
PMID: 24550007
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Rodgers B et al. (2014-01-01), Myostatin stimulates, not inihibits, C2C12 myoblast proliferation. Endocrinology, 2014-0155(3):670-5.
PMID: 24424069
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

Sneddon et al. (2012-10-07), Self-renewal of embryonic-stem-cell-derived progenitors by organ-matched mesenchyme. Nature, 2012-1491(7426):765-8.
PMID: 23041930
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Wilson TR et al. (2012-07-26), Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012-0487(7408):505-9.
PMID: 22763448
Sample Types: N/A
Applications: ELISA (Standard)

Diel P et al. (2010-08-27), Analysis of the effects of androgens and training on myostatin propeptide and follistatin concentrations in blood and skeletal muscle using highly sensitive Immuno PCR. Mol. Cell. Endocrinol., 2010-0330(1):1-9.
PMID: 20801187
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Wilkes JJ et al. (2009-02-10), Loss-of-function mutation in myostatin reduces tumor necrosis factor alpha production and protects liver against obesity-induced insulin resistance. Diabetes, 2009-058(5):1133-43.
PMID: 19208906
Species: Human
Sample Types: Recombinant Protein
Applications: ELISA Developmet, Surface Plasmon Resonance

Sengle G et al. (2008-07-02), A new model for growth factor activation: type II receptors compete with the prodomain for BMP-7. J. Mol. Biol., 2008-0381(4):1025-39.
PMID: 18621057
Species: Human
Sample Types: Recombinant Protein
Applications: Binding Assay, Surface Plasmon Resonance

Sengle G et al. (2008-03-13), Targeting of bone morphogenetic protein growth factor complexes to fibrillin. J. Biol. Chem., 2008-0283(20):13874-88.
PMID: 18339631
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Miller TM et al. (2006-12-12), Gene transfer demonstrates that muscle is not a primary target for non-cell-autonomous toxicity in familial amyotrophic lateral sclerosis. Proc. Natl. Acad. Sci. U.S.A., 2006-1103(51):19546-51.
PMID: 17164329
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Ohsawa Y et al. (2006-10-12), Muscular atrophy of caveolin-3-deficient mice is rescued by myostatin inhibition. J. Clin. Invest., 2006-1116(11):2924-34.
PMID: 17039257
Species: Chicken
Sample Types: Whole Tissue
Applications: Bioassay

Amthor H et al. (2004-06-01), Follistatin complexes Myostatin and antagonises Myostatin-mediated inhibition of myogenesis. Dev. Biol., 2004-0270(1):19-30.
PMID: 15136138

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FAQs for Recombinant Human/Mouse/Rat GDF-8/Myostatin Protein

Is Recombinant Human/Mouse/Rat GDF-8/Myostatin Protein (Catalog # 788-G8) the mature form of GDF8?

The mature form of GDF8 consists of a disulfide-linked homodimer. Catalog 788-G8 consists of Asp268-Ser376. There are multiple disulfide bonds within this sequence, which form a disulfide-linked heterodimer with another Asp268-Ser376 molecule.

Recombinant Human/Mouse/Rat GDF-8/Myostatin Protein Best Seller

Catalog # 788-G8 | R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Human/Mouse/Rat GDF-8/Myostatin Protein

Scientific Data Examples for Recombinant Human/Mouse/Rat GDF-8/Myostatin Protein

Formulation, Preparation and Storage

Carrier Free

788-G8

Carrier

788-G8/CF

Carrier Free

Reconstitution Calculator

Background: GDF-8/Myostatin

Citations for Recombinant Human/Mouse/Rat GDF-8/Myostatin Protein (40)

Customer Reviews for Recombinant Human/Mouse/Rat GDF-8/Myostatin Protein (2)

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Recombinant Human/Mouse/Rat GDF-8/Myostatin Protein

Recombinant Human/Mouse/Rat GDF-8/Myostatin Protein

FAQs for Recombinant Human/Mouse/Rat GDF-8/Myostatin Protein

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