Human/Mouse/Rat Galectin-3 Antibody

Name: Human/Mouse/Rat Galectin-3 Antibody
Brand: R&D Systems
SKU: AF1197

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF1197

Citations (21)

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Species Reactivity:	Human, Mouse, Rat
Immunogen:	E. coli-derived recombinant mouse Galectin‑3 Ala2-Ile264 Accession # NP_034835
Applications:	Immunohistochemistry, Simple Western, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects human, mouse, and rat Galectin-3 in Western blots. Detects mouse Galectin-3 in direct ELISAs. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) Galectin-3 is observed and less than 1% cross-reactivity with recombinant mouse (rm) Galectin-4 and rmGalectin-9 is observed.

View Full Technical Details for Human/Mouse/Rat Galectin-3 Antibody

Applications for Human/Mouse/Rat Galectin-3 Antibody

	Recommended Concentration	Sample
Western Blot	0.05-0.2 µg/mL	See below
Simple Western	10 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human/Mouse/Rat Galectin-3 Antibody

	Western Blot Detection of Mouse Galectin‑3 by Western Blot. Western blot shows lysates of 4T1 mouse breast cancer cell line, Balb/3T3 mouse embryonic fibroblast cell line and HT-2 mouse T cell line. PVDF membrane was probed with 0.05 µg/mL of Goat Anti-Mouse Galectin-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1197) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Galectin-3 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Western Blot Detection of Human and Rat Galectin‑3 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, U-118-MG human glioblastoma/astrocytoma cell line, C6 rat glioma cell line, and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse Galectin-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1197) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Galectin-3 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Immunohistochemistry Galectin‑3 in Mouse Embryo. Galectin-3 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c., cross-section through the eye) using Goat Anti-Human Galectin-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1197 ) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
	Simple Western Detection of Mouse Galectin‑3 by Simple Western^TM. Simple Western lane view shows lysates of HT-2 mouse T cell line and Balb/3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Galectin-3 at approximately 44 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse Galectin-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1197) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: Galectin-3

The galectins constitute a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified. The galectins have been classified into the prototype galectins (-1, -2, -5, -7, -10, -11, -13, -14), which contain one CRD and exist either as a monomer or a noncovalent homodimer; the chimera galectins (Galectin-3) containing one CRD linked to a nonlectin domain; and the tandem-repeat galectins (-4, -6, -8, -9, -12) consisting of two CRDs joined by a linker peptide. Galectins lack a classical signal peptide and can be localized to the cytosolic compartments where they have intracellular functions. However, via one or more as yet unidentified non-classical secretory pathways, galectins can also be secreted to function extracellularly. Individual members of the galectin family have different tissue distribution profiles and exhibit subtle differences in their carbohydrate-binding specificities. Each family member may preferentially bind to a unique subset of cell-surface glycoproteins (1-4).

Galectin-3, also known as Mac-2, L29, CBP35, and epsilon BP, is a chimera galectin that has a tendency to dimerize. Besides the soluble protein, alternatively spliced forms of chicken Galectin-3 containing a transmembrane-spanning domain and a leucine zipper motif have been reported. Galectin-3 is expressed in tumor cells, macrophages, activated T cells, osteoclasts, epithelial cells, and fibroblasts. It binds various matrix glycoproteins including laminin, fibronectin, LAMPS, 90K/Mac-2BP, MP20, and CEA. Galectin-3 promotes cell growth and proliferation for many cell types. Galectin-3 acts intracellularly to prevent apoptosis. Depending on the cell types, Galectin-3 exhibits pro- or anti-adhesive properties. Galectin-3 has proinflammatory activities in vitro and in vivo. It induces pro-inflammatory and inhibits Th2 type cytokine production. Galectin-3 chemoattracts monocytes and macrophages. It activates and degranulates basophils and mast cells. Elevated circulating levels of Galectin-3 has been show to correlate with the malignant potential of several types of cancer, suggesting that Galectin-3 is also involved in tumor growth and metastasis. Human and mouse Galectin-3 shares approximately 80% amino acid sequence similarity (1-5).

Item	Value
References	Rabinovich, A. et al. (2002) Trends in Immunol. 23:313. Rabinovich, A. et al. (2002) J. Leukocyte Biology 71:741. Hughes, R.C. (2001) Biochimie 83:667. R&D Systems Cytokine Bulletin; Summer 2002. Gorski, J.P. et al. (2002) J. Biol. Chem. 277:18840.
Entrez Gene IDs	3958 (Human); 16854 (Mouse)
Alternate Names	AGE-R3, CBP 35,35 kDa lectin, CBP35, Carbohydrate-binding protein 35, GAL3, GALBPCBP35, GALIG, Gal-3, Galactose-specific lectin 3, Galactoside-binding protein, Galectin-3, Galectin3, IgE-binding protein, L-31, L29, L31, LGALS2, LGALS3, Laminin-binding protein, Lectin L-29, MAC2GAL3, Mac-2, Mac-2 antigen, galactin-3, lectin, galactoside-binding, soluble, 3

Citations for Human/Mouse/Rat Galectin-3 Antibody (21)

Showing 1 - 10 of 21 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human/Mouse/Rat Galectin-3 Antibody include:

Species: Mouse
Sample Types: Whole Cells
Applications: ICC

T Wan et al. (2022), Astrocytic phagocytosis contributes to demyelination after focal cortical ischemia in mice Nature Communications, 13(1):1134.
PMID: 35241660
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

C Duarte et al. (2022), Crosstalk between dihydroceramides produced by Porphyromonas gingivalis and host lysosomal cathepsin B in the promotion of osteoclastogenesis Journal of Cellular and Molecular Medicine, 0(0).
PMID: 35429112
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

JC Fernández- et al. (2022), Gal3 Plays a Deleterious Role in a Mouse Model of Endotoxemia International Journal of Molecular Sciences, 23(3).
PMID: 35163089
Species: Transgenic Mouse
Sample Types: Cell Lysates
Applications: Western Blot

KA Maupin et al. (2022), Mutation of the galectin-3 glycan-binding domain (Lgals3-R200S) enhances cortical bone expansion in male mice and trabecular bone mass in female mice FEBS Open Bio, 0(0).
PMID: 36062328
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

H Shi et al. (2022), Regulating microglial miR-155 transcriptional phenotype alleviates Alzheimer's-induced retinal vasculopathy by limiting Clec7a/Galectin-3+ neurodegenerative microglia Acta neuropathologica communications, 10(1):136.
PMID: 36076283
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

NJ Esposito et al. (2021), Diurnal Photoreceptor Outer Segment Renewal in Mice Is Independent of Galectin-3 Investigative Ophthalmology Visual Science, 2021;62(2):7.
PMID: 33538769
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

C Emre et al. (2021), Age-related changes in brain phospholipids and bioactive lipids in the APP knock-in mouse model of Alzheimer's disease Acta neuropathologica communications, 9(1):116.
PMID: 34187579
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

YR Lee et al. (2020), Plasma APE1/Ref-1 Correlates with Atherosclerotic Inflammation in ApoE-/- Mice Biomedicines, 8(9).
PMID: 32967121
Species: Mouse
Sample Types: Protein
Applications: Western Blot

M Huang et al. (2020), Network analysis of the progranulin-deficient mouse brain proteome reveals pathogenic mechanisms shared in human frontotemporal dementia caused by GRN mutations Acta Neuropathol Commun, 8(1):163.
PMID: 33028409
Species: Human
Sample Types: Whole Tissue
Applications: IHC

CY Han et al. (2020), Serum amyloid A-containing HDL binds adipocyte-derived versican and macrophage-derived biglycan, reducing its anti-inflammatory properties JCI Insight, 0(0).
PMID: 32970631
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

M Svensson et al. (2020), Voluntary running does not reduce neuroinflammation or improve non-cognitive behavior in the 5xFAD mouse model of Alzheimer's disease Sci Rep, 10(1):1346.
PMID: 31992814
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

CY Han et al. (2020), Adipocyte-Derived Versican and Macrophage-Derived Biglycan Control Adipose Tissue Inflammation in Obesity Cell Rep, 31(13):107818.
PMID: 32610121
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

T Iwanaga et al. (2019), Bush-like integrin filament networks associated with hyaloid vasculature in murine neonate eyes Biomed. Res., 40(2):79-85.
PMID: 30982803
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

A Alawieh et al. (2018), Identifying the role of complement in triggering neuroinflammation after traumatic brain injury J. Neurosci., 0(0).
PMID: 29437855
I García-Dom et al. (2018), Peripheral Inflammation Enhances Microglia Response and Nigral Dopaminergic Cell Death in an in vivo MPTP Model of Parkinson's Disease Front Cell Neurosci, 12(0):398.
PMID: 30459561
A Alawieh et al. (2018), Acute Complement Inhibition Potentiates Neurorehabilitation and Enhances tPA-Mediated Neuroprotection J. Neurosci., 38(29):6527-6545.
PMID: 29921716
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

A Kishimoto et al. (2018), Histochemical characteristics of regressing vessels in the hyaloid vascular system of neonatal mice: Novel implication for vascular atrophy Exp. Eye Res., 172(0):1-9.
PMID: 29596849
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: Western Blot

(2016), Krï¿½ppel-like factor 3 (KLF3/BKLF) is required for widespread repression of the inflammatory modulator galectin-3 (Lgals3) J Biol Chem, 0(0).
PMID: 27226561
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Tsai CM et al. (2011), Galectin-1 and galectin-8 have redundant roles in promoting plasma cell formation. J. Immunol., 187(4):1643-52.
PMID: 21753146
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

Buhimschi CS et al. (2009), Characterization of RAGE, HMGB1, and S100beta in inflammation-induced preterm birth and fetal tissue injury. Am. J. Pathol., 175(3):958-75.
PMID: 19679874
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

Papaspyridonos M et al. (2007), Galectin-3 is an amplifier of inflammation in atherosclerotic plaque progression through macrophage activation and monocyte chemoattraction. Arterioscler. Thromb. Vasc. Biol., 28(3):433-40.
PMID: 18096829

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Human/Mouse/Rat Galectin-3 Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Human/Mouse/Rat Galectin-3 Antibody

Scientific Data Examples for Human/Mouse/Rat Galectin-3 Antibody

Western Blot

Western Blot

Immunohistochemistry

Simple Western

Formulation, Preparation and Storage

Reconstitution Calculator

Background: Galectin-3

Citations for Human/Mouse/Rat Galectin-3 Antibody (21)

Product Documents for Human/Mouse/Rat Galectin-3 Antibody

Product Datasheets

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