Human/Mouse/Rat Galectin-3 Antibody

Name: Human/Mouse/Rat Galectin-3 Antibody
Brand: R&D Systems
SKU: MAB1197

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB1197

Citations (4)

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Species Reactivity:	Human, Mouse, Rat
Immunogen:	E. coli-derived recombinant mouse Galectin‑3 Ala2-Ile264 Accession # P16110
Applications:	ELISA Capture, Simple Western, Western Blot
Label:	Unconjugated
Source:	Monoclonal Rat IgG_2A Clone # 202213
Specificity:	Detects human, mouse, and rat Galectin-3 in Western blots. Detects human and mouse Galectin-3 in direct ELISAs. In direct ELISAs, no cross-reactivity with rhGalectin-2, -4, -8 or recombinant mouse Galectin-1, -4, or -7 is observed.

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Applications for Human/Mouse/Rat Galectin-3 Antibody

	Recommended Concentration	Sample
Western Blot	0.25-0.5 µg/mL	See below
Simple Western	10 µg/mL	See below

Mouse Galectin-3 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Mouse Galectin‑3 Biotinylated Antibody (Catalog # BAF1197)

Standard: Recombinant Mouse Galectin-3 Protein (Catalog # 1197-GA)

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human/Mouse/Rat Galectin-3 Antibody

	Western Blot Detection of Mouse Galectin‑3 by Western Blot. Western blot shows lysates of 4T1 mouse breast cancer cell line, Balb/3T3 mouse embryonic fibroblast cell line and HT-2 mouse T cell line. PVDF membrane was probed with 0.25 µg/mL of Rat Anti-Mouse Galectin-3 Monoclonal Antibody (Catalog # MAB1197) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Galectin-3 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Western Blot Detection of Human and Rat Galectin‑3 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, U-118-MG human glioblastoma/astrocytoma cell line, C6 rat glioma cell line, and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse Galectin-3 Monoclonal Antibody (Catalog # MAB1197) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Galectin-3 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Simple Western Detection of Mouse Galectin‑3 by Simple Western^TM. Simple Western lane view shows lysates of HT-2 mouse T cell line and Balb/3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Galectin-3 at approximately 43 kDa (as indicated) using 10 µg/mL of Rat Anti-Mouse Galectin-3 Monoclonal Antibody (Catalog # MAB1197) followed by 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: Galectin-3

The galectins constitute a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified. The galectins have been classified into the prototype galectins (-1, -2, -5, -7, -10, -11, -13, -14), which contain one CRD and exist either as a monomer or a noncovalent homodimer; the chimera galectins (Galectin-3) containing one CRD linked to a nonlectin domain; and the tandem-repeat galectins (-4, -6, -8, -9, -12) consisting of two CRDs joined by a linker peptide. Galectins lack a classical signal peptide and can be localized to the cytosolic compartments where they have intracellular functions. However, via one or more as yet unidentified non-classical secretory pathways, galectins can also be secreted to function extracellularly. Individual members of the galectin family have different tissue distribution profiles and exhibit subtle differences in their carbohydrate-binding specificities. Each family member may preferentially bind to a unique subset of cell-surface glycoproteins (1-4). Galectin-3, also known as Mac-2, L29, CBP35, and epsilon BP, is a chimera galectin that has a tendency to dimerize. Besides the soluble protein, alternatively spliced forms of chicken Galectin-3 containing a transmembrane-spanning domain and a leucine zipper motif have been reported. Galectin-3 is expressed in tumor cells, macrophages, activated T cells, osteoclasts, epithelial cells, and fibroblasts. It binds various matrix glycoproteins including laminin, fibronectin, LAMPS, 90K/Mac-2BP, MP20, and CEA. Galectin-3 promotes cell growth and proliferation for many cell types. Galectin-3 acts intracellularly to prevent apoptosis. Depending on the cell types, Galectin-3 exhibits pro- or anti-adhesive properties. Galectin-3 has proinflammatory activities in vitro and in vivo. It induces pro-inflammatory and inhibits Th2 type cytokine production. Galectin-3 chemoattracts monocytes and macrophages. It activates and degranulates basophils and mast cells. Elevated circulating levels of Galectin-3 has been show to correlate with the malignant potential of several types of cancer, suggesting that Galectin-3 is also involved in tumor growth and metastasis. Human and mouse Galectin-3 shares approximately 80% amino acid sequence similarity (1-5).

Item	Value
References	Rabinovich, A. et al. (2002) Trends in Immunol. 23:313. Rabinovich, A. et al. (2002) J. Leukocyte Biology 71:741. Hughes, R.C. (2001) Biochimie 83:667. R&D Systems Cytokine Bulletin; Summer 2002. Gorski, J.P. et al. (2002) J. Biol. Chem. 277:18840.
Entrez Gene IDs	3958 (Human); 16854 (Mouse)
Alternate Names	AGE-R3, CBP 35,35 kDa lectin, CBP35, Carbohydrate-binding protein 35, GAL3, GALBPCBP35, GALIG, Gal-3, Galactose-specific lectin 3, Galactoside-binding protein, Galectin-3, Galectin3, IgE-binding protein, L-31, L29, L31, LGALS2, LGALS3, Laminin-binding protein, Lectin L-29, MAC2GAL3, Mac-2, Mac-2 antigen, galactin-3, lectin, galactoside-binding, soluble, 3

Citations for Human/Mouse/Rat Galectin-3 Antibody (4)

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Citations are publications that use Bio-Techne products. Selected citations for Human/Mouse/Rat Galectin-3 Antibody include:

Species: Rat
Sample Types: Hippocampal Cell Lysates
Applications: Western Blot

CC Tao et al. (2020), Galectin-3 promotes A&beta oligomerization and A&beta toxicity in a mouse model of Alzheimer's disease Cell Death Differ., 27(1):192-209.
PMID: 31127200
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

T Clemente et al. (2017), Proteomic and functional analysis identifies galectin-1 as a novel regulatory component of the cytotoxic granule machinery Cell Death Dis, 8(12):e3176.
PMID: 29215607
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

Fermino M et al. (2013), Galectin-3 negatively regulates the frequency and function of CD4(+) CD25(+) Foxp3(+) regulatory T cells and influences the course of Leishmania major infection. Eur J Immunol, 43(7):1806-17.
PMID: 23592449
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization

(2012), The receptor of advanced glycation end products plays a central role in advanced oxidation protein products-induced podocyte apoptosis. Kidney Int, 82(7):759-70.
PMID: 22622498

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Human/Mouse/Rat Galectin-3 Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Human/Mouse/Rat Galectin-3 Antibody

Mouse Galectin-3 Sandwich Immunoassay

Scientific Data Examples for Human/Mouse/Rat Galectin-3 Antibody

Western Blot

Western Blot

Simple Western

Formulation, Preparation and Storage

Reconstitution Calculator

Background: Galectin-3

Citations for Human/Mouse/Rat Galectin-3 Antibody (4)

Product Documents for Human/Mouse/Rat Galectin-3 Antibody

Product Datasheets

COA

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