Human/Mouse/Rat FAK Antibody

Name: Human/Mouse/Rat FAK Antibody
Brand: rnd
SKU: AF4467

Catalog # AF4467

Citations (2)

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Key Product Details

Species Reactivity:	Human, Mouse, Rat
Immunogen:	E. coli-derived recombinant human FAK Asp213-Thr412 Accession # Q05397
Applications:	Immunohistochemistry, Knockout Validated, Western Blot
Label:	Unconjugated
Source:	Polyclonal Sheep IgG
Specificity:	Detects human, mouse and rat FAK.

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Applications

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	See below

Item	Value
Knockout Validated	FAK is specifically detected in HEK293T human embryonic kidney parental cell line but is not detectable in FAK knockout HEK293T cell line.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Human/Mouse/Rat FAK Antibody Scientific Data Examples

	Western Blot Detection of Human, Mouse, and Rat FAK by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line, HUVEC human umbilical vein endothelial cells, Neuro‑2A mouse neuroblastoma cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat FAK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4467) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for FAK at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
	Immunohistochemistry FAK in Human Brain. FAK was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using 3 µg/mL Human/Mouse/Rat FAK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4467) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
	Knockout Validated Western Blot Shows Human FAK Specificity by Using Knockout Cell Line. Western blot shows lysates of HEK293T human embryonic kidney parental cell line and FAK knockout HEK293T cell line (KO). PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat FAK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4467) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FAK at approximately 135 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: FAK

Focal adhesion kinase 1 (FAK) is a ubiquitously expressed non-receptor protein tyrosine kinase that is concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. This cellular localization is directed by a "Focal Adhesion Targeting" (FAT) sequence, a 125 amino acid sequence at the C-terminus. FAK plays an important role in migration, cell spreading, differentiation, cytoskeleton protein phosphorylation, apoptosis and acceleration of the G1 to S phase transition of the cell cycle. It associates with several different signaling proteins such as Src-family PTKs, p130Cas, Shc, Grb2, PI 3-kinase, and paxillin. This enables FAK to function within a network of integrin-stimulated signaling pathways leading to the activation of targets such as the ERK and JNK/mitogen-activated protein kinase pathways. FAK is also linked to oncogenes at biochemical and functional levels. Increased expression and/or activity of FAK in various tumors has been correlated with enhanced migration and invasiveness of human tumor cells in addition to promoting increased cell proliferation.

Item	Value
Long Name	Focal adhesion kinase 1
Entrez Gene IDs	5747 (Human); 14083 (Mouse); 25614 (Rat)
Alternate Names	EC 2.7.10, FADK 1, FADK1, FADKFAK-related non-kinase polypeptide, FAK, FAK1FRNK, FAKEC 2.7.10.2, PTK2, PTK2 protein tyrosine kinase 2, Protein-tyrosine kinase 2, focal adhesion kinase 1, pp125FAK

Citations for Human/Mouse/Rat FAK Antibody (2)

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Citations are publications that use Bio-Techne products. Selected citations for Human/Mouse/Rat FAK Antibody include:

Species: Rat
Sample Types: Tissue Homogenates
Applications: ELISA Development (Capture)

C Ferrié et al. (2019), Focal adhesion kinase coordinates costamere-related JNK signaling with muscle fiber transformation after Achilles tenotomy and tendon reconstruction Exp. Mol. Pathol., 0(0).
PMID: 30879953
Species: Human
Sample Types: Tissue Homogenates
Applications: Immunoprecipitation

M Flück et al. (2018), Knee Extensors Muscle Plasticity Over a 5-Years Rehabilitation Process After Open Knee Surgery Front Physiol, 9(0):1343.
PMID: 30337877

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