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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # CTC5

Product Summary for Human/Mouse/Rat CCR5 PE‑conjugated Antibody

Immunogen

CHO Chinese hamster ovary cell line transfected with human CCR5

Specificity

Detects human CCR5 in Western blots. For additional information regarding epitope specificity for this antibody and other R&D Systems anti‑human CCR5 antibodies, see Reference 1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Mouse/Rat CCR5 PE‑conjugated Antibody

Detection of CCR5 antibody in Human PBMCs antibody by Flow Cytometry.

Detection of CCR5 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3e Alexa Fluor® 405-conjugated Monoclonal Antibody (Catalog # FAB100V) and either (A) Mouse Anti-Human/Mouse/Rat CCR5 PE-conjugated Monoclonal Antibody (Catalog # FAB1802P) or (B) Mouse IgG1Phycoerythrin Isotype Control (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.
Detection of CCR5 antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of CCR5 in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes treated with anti-CD3, anti-CD28, 5 ng/mL Recombinant Mouse IL-12 (Catalog # 419-ML) and 10 µg/mL Goat Anti-Mouse IL-4 Polyclonal Antibody (Catalog # AB-404-NA) for 3 days to induce Th1 development were stained with Mouse Anti-Human/Mouse/Rat CCR5 PE-conjugated Monoclonal Antibody (Catalog # FAB1802P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of CCR5 antibody in Rat Splenocytes antibody by Flow Cytometry.

Detection of CCR5 in Rat Splenocytes by Flow Cytometry.

Rat splenocytes were stained with Mouse Anti-Human/Mouse/Rat CCR5 PE-conjugated Monoclonal Antibody (Catalog # FAB1802P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human/Mouse/Rat CCR5 PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs), mouse splenocytes treated with anti-CD3, anti-CD28, Recombinant Mouse IL‑12 (Catalog # 419-ML) and Goat Anti-Mouse IL‑4 Polyclonal Antibody (Catalog # AB-404-NA) to induce Th1 development, and rat splenocytes
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4 using FAB1802P in the following applications:

Published Applications

Read 7 publications using FAB1802P in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR5

CCR5 is a G protein-linked seven transmembrane domain chemokine receptor. CCR5 serves as a receptor for several chemokines including MIP-1 alpha, MIP-1 beta, MCP-2, and RANTES. It also functions as a coreceptor for Macrophage Tropic HIV-1 infection.

References

  1. Lee, B. et al. (1999) J. Biol. Chem. 274:9617.

Alternate Names

CCR5, CD195

Entrez Gene IDs

1234 (Human); 12774 (Mouse); 117029 (Rat); 484789 (Canine); 493769 (Feline)

Gene Symbol

CCR5

Product Documents for Human/Mouse/Rat CCR5 PE‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse/Rat CCR5 PE‑conjugated Antibody

For research use only

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