Human/Mouse/Rat CCR5 APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB1802A
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Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Rat
Applications
Validated:
Flow Cytometry
Cited:
Flow Cytometry, Immunocytochemistry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # CTC5
Product Specifications
Immunogen
CHO Chinese hamster ovary cell line transfected with human CCR5
Specificity
Detects human CCR5 in Western blots. For additional information regarding epitope specificity for this antibody and other R&D Systems anti‑human CCR5 antibodies, see Reference 1.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human/Mouse/Rat CCR5 APC-conjugated Antibody
Detection of CCR5 in Human PBMCs by Flow Cytometry.
Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3e Alexa Fluor® 405-conjugated Monoclonal Antibody (Catalog # FAB100V) and either (A) Mouse Anti-Human/Mouse/Rat CCR5 APC-conjugated Monoclonal Antibody (Catalog # FAB1802A) or (B) Mouse IgG1Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.
Detection of CCR5 in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes treated with anti-CD3, anti-CD28, 5 ng/mL Recombinant Mouse IL-12 (Catalog # 419-ML) and 10 µg/mL Goat Anti-Mouse IL-4 Poly-clonal Antibody (Catalog # AB-404-NA) to induce Th1 development for 3 days were stained with Mouse Anti-Human/Mouse/Rat CCR5 APC-conjugated Monoclonal Antibody (Catalog # FAB1802A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of CCR5 in Rat Splenocytes by Flow Cytometry.
Rat splenocytes were stained with Mouse Anti-Human/Mouse/Rat CCR5 APC-conjugated Monoclonal Antibody (Catalog # FAB1802A) and Anti-Rat CD3 PE-conjugated Antibody. Quadrant markers were set based on control antibody staining (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.Applications for Human/Mouse/Rat CCR5 APC-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs), mouse splenocytes treated with anti-CD3, anti-CD28, Recombinant Mouse IL‑12 (Catalog # 419-ML) and Goat Anti-Mouse IL‑4 Polyclonal Antibody (Catalog # AB-404-NA) to induce Th1 development, and rat splenocytes
Sample: Human peripheral blood mononuclear cells (PBMCs), mouse splenocytes treated with anti-CD3, anti-CD28, Recombinant Mouse IL‑12 (Catalog # 419-ML) and Goat Anti-Mouse IL‑4 Polyclonal Antibody (Catalog # AB-404-NA) to induce Th1 development, and rat splenocytes
Reviewed Applications
Read 2 reviews rated 5 using FAB1802A in the following applications:
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Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CCR5
CCR5 is a G protein-linked seven transmembrane domain chemokine receptor. CCR5 serves as a receptor for several chemokines including MIP-1 alpha, MIP-1 beta, MCP-2, and RANTES. It also functions as a coreceptor for Macrophage Tropic HIV-1 infection.
References
- Lee, B. et al. (1999) J. Biol. Chem. 274:9617.
Alternate Names
CCR5, CD195
Gene Symbol
CCR5
Additional CCR5 Products
Product Documents for Human/Mouse/Rat CCR5 APC-conjugated Antibody
Product Specific Notices for Human/Mouse/Rat CCR5 APC-conjugated Antibody
For research use only
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