Skip to main content

Human/Mouse/Rat 14-3-3 gamma Antibody

Bio-Techne includes R&D Systems | Catalog # MAB5700

Catalog #
Availability
Size / Price
Qty
Loading...
MAB5700
MAB5700-SP

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 505810

Product Summary for Human/Mouse/Rat 14-3-3 gamma Antibody

Immunogen

E. coli-derived recombinant human 14-3-3 gamma
Met1-Asn247
Accession # P61981

Specificity

Detects human, mouse, and rat 14-3-3 gamma in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human/Mouse/Rat 14-3-3 gamma Antibody

Detection of Human, Mouse, and Rat 14-3-3? antibody by Western Blot.

Detection of Human, Mouse, and Rat 14‑3‑3 gamma by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat 14-3-3 gamma Monoclonal Antibody (Catalog # MAB5700) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for 14-3-3 gamma was detected at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human 14-3-3? antibody by Western Blot.

Detection of Human 14-3-3 gamma by Western Blot.

Western blot shows recombinant human 14-3-3 beta, epsilon, eta, gamma, sigma, theta, and zeta (5 ng/lane. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat 14-3-3 gamma Monoclonal Antibody (Catalog # MAB5700) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for 14-3-3 gamma was detected at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat 14-3-3 gamma Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty
Loading...

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 14-3-3 gamma

14-3-3 proteins are a highly conserved family of homo- and heterodimeric phosphoserine/threonine-binding proteins present in all eukaryotic cells. 14-3-3 proteins act as key regulators of intracellular signal transduction through their ability to bind specific motifs phosphorylated on serine or threonine. There are at least 7 distinct mammalian 14-3-3 genes: alpha/ beta, epsilon, eta, gamma, tau/ theta, sigma, and zeta/ delta. 14-3-3 gamma becomes phosphorylated in response to PDGF and subsequently interacts with different PKC isoforms.

Alternate Names

1433 gamma, YWHAG

Entrez Gene IDs

7532 (Human); 22628 (Mouse); 56010 (Rat)

Gene Symbol

YWHAG

UniProt

Additional 14-3-3 gamma Products

Product Documents for Human/Mouse/Rat 14-3-3 gamma Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse/Rat 14-3-3 gamma Antibody

For research use only

Loading...
Loading...
Loading...
Loading...