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Human/Mouse Bax Antibody
R&D Systems, Inc. a Bio-Techne Brand
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Key Product Details
| Species Reactivity: | Human, Mouse |
| Immunogen: | KLH-coupled human Bax synthetic peptide |
| Applications: | Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Knockout Validated, Simple Western, Western Blot |
| Label: | Unconjugated |
| Source: | Polyclonal Rabbit IgG |
| Specificity: |
Detects human and mouse Bax. |
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Western BlotDetection of Human/Mouse Bax by Western Blot. Western blot shows lysates of CHP-100 human neuroblastoma cell line, THP-1 human acute monocytic leukemia cell line, L-929 mouse fibroblast cell line, and DA3 mouse myeloma cell line. PVDF membrane was probed with 0.3 µg/mL of Rabbit Anti-Human/Mouse Bax Antigen Affinity-purified Polyclonal Antibody (Catalog # AF820) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Bax at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2. |
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ImmunocytochemistryBax in A549 Human Cell Line. Bax was detected in immersion fixed A549 human lung carcinoma cell line using Rabbit Anti-Human/Mouse Bax Antigen Affinity-purified Polyclonal Antibody (Catalog # AF820) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
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ImmunohistochemistryBax in Human Breast. Bax was detected in immersion fixed paraffin-embedded sections of human breast using 15 µg/mL Rabbit Anti-Human/Mouse Bax Antigen Affinity-purified Polyclonal Antibody (Catalog # AF820) overnight at 4 °C. Tissue was stained (red) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
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ImmunohistochemistryBax in Human Liver. Bax was detected in immersion fixed paraffin-embedded sections of human liver using 10 µg/mL Rabbit Anti-Human/Mouse Bax Antigen Affinity-purified Polyclonal Antibody (Catalog # AF820) overnight at 4 °C. Tissue was stained with the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
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Knockout ValidatedWestern Blot Shows Human Bax Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Bax knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse Bax Antigen Affinity-purified Polyclonal Antibody (Catalog # AF820) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Bax at approximately 20 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
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Simple WesternDetection of Human Bax by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Bax knockout HeLa cell line (KO), loaded at 0.2 mg/mL. A specific band was detected for Bax at approximately 26 kDa (as indicated) using 50 µg/mL of Rabbit Anti-Human/Mouse Bax Antigen Affinity-purified Polyclonal Antibody (Catalog # AF820). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
Citations for Human/Mouse Bax Antibody (7)
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Citations are publications that use Bio-Techne products. Selected citations for Human/Mouse Bax Antibody include:
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Species: Rat
Sample Types: Tissue Lysates
Applications: Western BlotH Wang et al. (2022), Human Umbilical Cord Mesenchymal Stem Cells Attenuate Severe Burn-Induced Multiple Organ Injury via Potentiating IGF-1 and BCL-2/BAX Pathway Stem Cells International, 2022(0):5474289.
PMID: 36591374 -
Species: Rat
Sample Types: Cell Lysates
Applications: Western BlotK Hira et al. (2021), Cyclo(Val-Pro) and Cyclo(Leu-Hydroxy-Pro) from Pseudomonas sp. (ABS-36) alleviates acute and chronic renal injury under in vitro and in vivo models (Ischemic reperfusion and unilateral ureter obstruction) International immunopharmacology, 103(0):108494.
PMID: 34973530 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotX Ma et al. (2019), Lipid storage droplet protein 5 reduces sodium palmitate?induced lipotoxicity in human normal liver cells by regulating lipid metabolism?related factors Mol Med Rep, 20(2):879-886.
PMID: 31173228 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P(2016), Bcl-xL Is Essential for the Survival and Function of Differentiated Neurons in the Cortex That Control Complex Behaviors J Neurosci, 36(20):5448-61.
PMID: 27194326 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotKarrar A et al. (2006), Human liver sinusoidal endothelial cells induce apoptosis in activated T cells: a role in tolerance induction. Gut, 56(2):243-52.
PMID: 16840505 -
Species: Rat
Sample Types: Cell Lysates
Applications: Western BlotXi H et al. (2004), Sustained early growth response gene 3 expression inhibits the survival of CD4/CD8 double-positive thymocytes. J. Immunol., 173(1):340-8.
PMID: 15210792 -
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western BlotJayanthi S et al. (2001), Methamphetamine causes differential regulation of pro-death and anti-death Bcl-2 genes in the mouse neocortex. , 15(10):1745-52.
PMID: 11481222
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Customer Reviews for Human/Mouse Bax Antibody (2)
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Application: ELISASample Tested: Cancer cell lysatesSpecies: HumanVerified Customer | Posted 07/30/2019
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Application: WBSample Tested: MDA-MB-231 human breast cancer cell lineSpecies: HumanVerified Customer | Posted 01/11/2018
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