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Human MMR/CD206 Antibody

Bio-Techne includes R&D Systems | Catalog # AF2534

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AF2534
AF2534-SP

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry, Immunohistochemistry, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Summary for Human MMR/CD206 Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant human MMR/CD206
Leu19-Lys1383 (Thr399Ala) & (Leu407Phe)
Accession # P22897

Specificity

Detects human MMR/CD206 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse MMR is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human MMR/CD206 Antibody

Detection of Human MMR/CD206 antibody by Western Blot.

Detection of Human MMR/CD206 by Western Blot.

Western blot shows lysates of human immature dendritic cells. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human MMR/CD206 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2534) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MMR/CD206 at approximately 185 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

MMR/CD206 in Human Liver.

MMR/CD206 was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human MMR/CD206 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2534) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sinusoids. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Human MMR/CD206 by Simple WesternTM.

Simple Western lane view shows lysates of human immature dendritic cells, loaded at 0.2 mg/mL. A specific band was detected for MMR/CD206 at approximately 201 kDa (as indicated) using 10 µg/mL of Goat Anti-Human MMR/CD206 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2534) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human MMR/CD206 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells

Immunohistochemistry

3-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver

Simple Western

10 µg/mL
Sample: Human immature dendritic cells

Western Blot

1 µg/mL
Sample: Human immature dendritic cells
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 4 reviews rated 3.8 using AF2534 in the following applications:

Published Applications

Read 36 publications using AF2534 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMR/CD206

The human Macrophage Mannose Receptor (MMR), also known as CD206 and MRC1 (mannose receptor C, type 1), is a 190 kDa scavenger receptor that is expressed on tissue macrophages, myeloid dendritic cells, and liver and lymphatic endothelial cells (1). It belongs to a family of receptors sharing similar protein structure that also includes DEC205, phospholipase A2 receptor, and Endo180 (2, 3). The human MMR protein is synthesized as a 1456 amino acid (aa) precursor that contains an 18 aa signal sequence, a 1371 aa extracellular region, a 21 aa transmembrane segment and a 46 aa cytoplasmic domain (4). Its extracellular region is composed of an N‑terminal cysteine-rich domain, followed by a single fibronectin type II repeat, and eight C-type lectin carbohydrate recognition domains (CRD) (3, 4). Human to mouse, the extracellular region is 82% aa identical. The cysteine-rich domain mediates recognition of sulfated N‑acetylgalactosamine, which occurs on some extracellular matrix proteins and is the terminal sugar of the unusual oligosaccharides present on pituitary hormones such as lutropin and thyrotropin (5). Several of the CRDs participate in the Ca2+-dependent recognition of carbohydrates showing a preference for branched sugars with terminal mannose, fucose or N‑acetylglucosamine (6). The cytoplasmic domain of MMR includes a tyrosine-based motif for internalization in clathrin-coated vesicles. Once internalized, ligands are released following acidification of phagosomes or endosomes, and the receptor is recycled to the cell surface (3, 7). MMR mediates phagocytosis upon binding to target structures that occur on a variety of pathogenic microorganisms including Gram-negative and Gram-positive bacteria, yeasts, parasites, and mycobacteria. MMR also functions to maintain homeostasis through the endocytosis of potentially harmful glycoproteins associated with inflammation (2, 3).

References

  1. East, L. and C. Isake (2002) Biochim. Biophys. Acta 1572:364. 
  2. Chieppa, M. et al. (2003) J. Immunol. 171:4552. 
  3. Figdor, C. et al. (2002) Nat. Rev. Immunol. 2:77.
  4. Taylor, M. et al. (1990) J. Biol. Chem. 265:12156.
  5. Leteux, C. et al. (2000) J. Exp. Med. 191:1117.
  6. Martinez-Pomares, L. et al. (2001) Immunobiology 204:527.
  7. Feinberg, H. et al. (2000) J. Biol. Chem. 275:21539.

Long Name

Macrophage Mannose Receptor

Alternate Names

CD206, CLEC13D, MRC1

Entrez Gene IDs

4360 (Human); 17533 (Mouse); 291327 (Rat)

Gene Symbol

MRC1

UniProt

Additional MMR/CD206 Products

Product Documents for Human MMR/CD206 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human MMR/CD206 Antibody

For research use only

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