Human M-CSF Antibody

Name: Human M-CSF Antibody
Brand: R&D Systems
SKU: MAB616
Rating: 5 (2 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB616

Citations (2)

(2)

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Key Product Details

Species Reactivity:	Human
Immunogen:	E. coli-derived recombinant human M-CSF
Applications:	ELISA Capture, Western Blot
Label:	Unconjugated
Source:	Monoclonal Mouse IgG_2A Clone # 21113
Specificity:	Detects recombinant human (rh)M-CSF in Western blots. In sandwich ELISAs, less than 7% cross-reactivity was observed with rmM-CSF. No significant cross-reactivity or interference was observed with over 75 other cytokines tested.

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Applications for Human M-CSF Antibody

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Human M-CSF (Catalog # 216-MC) under non-reducing conditions only

Human M-CSF Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human M‑CSF Biotinylated Antibody (Catalog # BAF216)

Standard: Recombinant Human M-CSF Protein (Catalog # 216-MC)

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Protein A or G purified from ascites
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: M-CSF

M-CSF, also known as CSF-1, is a four-alpha-helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation. M-CSF is also essential for the survival and proliferation of osteoclast progenitors. M-CSF also primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis. M-CSF increases during pregnancy to support implantation and growth of the decidua and placenta. Sources of M-CSF include fibroblasts, activated macrophages, endometrial secretory epithelium, bone marrow stromal cells and activated endothelial cells. The M-CSF receptor (c-fms) transduces its pleotropic effects and mediates its endocytosis. M-CSF mRNAs of various sizes occur. Full length human M-CSF transcripts encode a 522 amino acid (aa) type I transmembrane (TM) protein with a 464 aa extracellular region, a 21 aa TM domain, and a 37 aa cytoplasmic tail that forms a 140 kDa covalent dimer. Differential processing produces two proteolytically cleaved, secreted dimers. One is an N- and O- glycosylated 86 kDa dimer, while the other is modified by both glycosylation and chondroitin-sulfate proteoglycan (PG) to generate a 200 kDa subunit. Although PG-modified M-CSF can circulate, it may be immobilized by attachment to type V collagen. Shorter transcripts encode M-CSF that lack cleavage and PG sites and produce an N-glycosylated 68 kDa TM dimer and a slowly produced 44 kDa secreted dimer. Although forms may vary in activity and half-life, all contain the N-terminal 150 aa portion that is necessary and sufficient for interaction with the M-CSF receptor. The first 223 aa of mature human M-CSF shares 88%, 86%, 81% and 74% aa identity with corresponding regions of dog, cow, mouse and rat M-CSF, respectively. Human M-CSF is active in the mouse, but mouse M-CSF is reported to be species-specific.

Item	Value
Long Name	Macrophage Colony Stimulating Factor
Entrez Gene IDs	1435 (Human); 12977 (Mouse)
Alternate Names	CSF-1, CSF1, Lanimostim, M-CSF, MCSF, MCSFlanimostim, MGC31930, colony stimulating factor 1 (macrophage), macrophage colony stimulating factor, macrophage colony-stimulating factor 1

Citations for Human M-CSF Antibody (2)

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Citations are publications that use Bio-Techne products. Selected citations for Human M-CSF Antibody include:

Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Capture

D Bayik et al. (2018), Factors Influencing the Differentiation of Human Monocytic Myeloid-Derived Suppressor Cells Into Inflammatory Macrophages Front Immunol, 9(0):608.
PMID: 29632539
Species: Human
Sample Types: Cell Culture Supernates
Applications: Luminex Development

Schipper HS et al. (2010), A multiplex immunoassay for human adipokine profiling. Clin. Chem., 56(0):1320.
PMID: 20530731

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Customer Reviews for Human M-CSF Antibody (2)

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Human M-CSF Antibody

Name: Anonymous

Application: ELISA

Sample Tested: EDTA Plasma

Species: Human

Verified Customer | Posted 07/23/2019
Human M-CSF Antibody

Name: Anonymous

Application: ELISA

Sample Tested: Serum and Plasma

Species: Human

Verified Customer | Posted 11/03/2017

This antibody was used as a capture antibody in the development of a sandwich ELISA. The R&D systems polyclonal antibody AF216 was used as the detection antibody. M-CSF was measured in human serum samples with this assay.

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Human M-CSF Antibody

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Applications for Human M-CSF Antibody

Human M-CSF Sandwich Immunoassay

Formulation, Preparation and Storage

Reconstitution Calculator

Background: M-CSF

Citations for Human M-CSF Antibody (2)

Customer Reviews for Human M-CSF Antibody (2)

Customer Images

Human M-CSF Antibody

Human M-CSF Antibody

Product Documents for Human M-CSF Antibody

Product Datasheets

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