Key Product Details

Species Reactivity

Human

Applications

Western Blot, Intracellular Staining by Flow Cytometry, Immunocytochemistry, Simple Western, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 693010

View all Lck Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Lck
Met1-Val66
Accession # P06239

Specificity

Detects human Lck in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant rat Lck is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Lck Antibody

Detection of Human Lck antibody by Western Blot.

Detection of Human Lck by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line and COLO 205 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Lck Monoclonal Antibody (Catalog # MAB37041) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for Lck at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Lck antibody in Human Blood Lymphocytes antibody by Flow Cytometry.

Detection of Lck in Human Blood Lymphocytes by Flow Cytometry.

Human peripheral blood lymphocytes were stained with Mouse Anti-Human Lck Monoclonal Antibody (Catalog # MAB37041) followed by Phycoery-thrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (FAB100A). Quadrant markers were set based on control antibody staining (MAB003).
Lck antibody in Human PBMCs by Immunocytochemistry (ICC).

Lck in Human PBMCs.

Lck was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human Lck Monoclonal Antibody (Catalog # MAB37041) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
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Applications for Human Lck Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs)

Intracellular Staining by Flow Cytometry

2.5 µg/106 cells
Sample: Human peripheral blood lymphocytes fixed with paraformaldehyde and permeabilized with saponin

Simple Western

2 µg/mL
Sample: Jurkat human acute T cell leukemia cell line

Western Blot

0.2 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and COLO 205 human colorectal adenocarcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lck

Lck (p56lck) is a 56 kDa cytosolic phosphoprotein within the Src family of non-receptor tyrosine kinases. The 509 amino acid (aa) human Lck contains an SH3 domain (aa 61-121), an SH2 domain (aa 127-224) and a protein kinase domain (aa 251-259). Within aa 2-66, human Lck shares 89% aa sequence identity with mouse and rat Lck. A short (363 aa) isoform contains alternate sequence starting at aa 348, while a 539 aa isoform contains inserted sequence after aa 321. Lck interacts with T cell CD4 and CD8 molecules and plays a pivotal role in regulating T cell activation. It can be overexpressed in cancers and functions as an oncogene.

Long Name

Lymphocyte Protein Tyrosine Kinase

Alternate Names

Hck-3, Lcktkr, LSTRA, p56lck, pp58lck, YT16

Entrez Gene IDs

3932 (Human); 16818 (Mouse); 313050 (Rat)

Gene Symbol

LCK

UniProt

P06239

Additional Lck Products

Product Documents for Human Lck Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Lck Antibody

For research use only

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