Human Jarid1A/KDM5A Antibody

Detection of Human Jarid1A by Western Blot.

Western Blot shows lysates of NTera-2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human Jarid1A Monoclonal Antibody (Catalog # MAB11705) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Jarid1A at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of Human Jarid1A by Simple Western^TM.

Left: Simple Western lane view shows lysates of NTera-2 human testicular embryonic carcinoma cell line, loaded at 0.75 mg/ml. A specific band was detected for Jarid1A at approximately 191 kDa (as indicated) using both 2 µg/ml and 10 µg/ml of Mouse Anti-Human Jarid1A Monoclonal Antibody (Catalog # MAB11705) followed by HRP-conjugated Goat Anti-Mouse Secondary Antibody (Catalog # 042-205). This experiment was conducted under reducing conditions and using the 66-440kDa separation system. Right: Simple Western electropherogram showing the same Mouse Anti-Human Jarid1A Monoclonal Antibody (Catalog # MAB11705) tested at 2 µg/ml (blue line) and 10 µg/ml (green line) in the NTera-2 human testicular embryonic carcinoma cell line.