Detects human Integrin beta8 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Integrins beta1, beta2, beta3, beta4, beta5, beta6, beta7, or recombinant mouse Integrin beta8 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human Integrin beta8 Antibody

Detection of Integrin beta8 in A172 Human Cell Line by Flow Cytometry.

WM-115 human melanoma cell line was stained with Human Integrin beta8 Monoclonal Antibody (Catalog # MAB4775, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Integrin beta8 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: WM-115 human melanoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin beta 8

Integrin beta 8 (Integrin beta8) is a 90 kDa type I transmembrane glycoprotein of the Integrin family of adhesion molecules. It associates with Integrin alphaV to form a receptor for vitronectin, fibrin, and the latency associated peptide (LAP). Binding to LAP promotes the proteolytic release of active TGF-beta from LAP. Integrin alphaV beta8 is required for vascular morphogenesis in the embryonic brain and yolk sac. Within the extracellular domain, human Integrin beta8 shares 87% aa sequence identity with mouse and rat Integrin beta8.

Alternate Names

ITGB8

Entrez Gene IDs

3696 (Human); 320910 (Mouse); 362800 (Rat)

Gene Symbol

ITGB8

UniProt

P26012

Additional Integrin beta 8 Products

Product Documents for Human Integrin beta8 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Integrin beta8 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Integrin beta8 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4775

Key Product Details

Species Reactivity

Validated:

Cited:

Applications