Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Neutralization, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation, Bioassay, Blocking, ELISA Capture, Flow Cytometery

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # P5D2

View all Integrin beta 1/CD29 Antibodies »

Product Specifications

Immunogen

Human skin keratinocytes

Specificity

Detects human Integrin beta1/CD29.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human Integrin beta1/CD29 Antibody

Detection of Integrin beta1/CD29 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human Integrin beta1/CD29 Monoclonal Antibody (Catalog # MAB17781, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Detection of Integrin beta1/CD29 in A549 human lung carcinoma cell line by Flow Cytometry.

A549 human lung carcinoma cell line was stained with Mouse Anti-Human Integrin beta1/CD29 Monoclonal Antibody (Catalog # MAB17781, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Integrin beta1/CD29 in human PBMCs.

Integrin beta1/CD29 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human Integrin beta1/CD29 Monoclonal Antibody (Catalog # MAB17781) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Integrin beta1/CD29 Specificity is Shown by Flow Cytometry in Knockout Cell Line.

Integrin beta1/CD29 knockout A549 human lung carcinoma cell line was stained with Mouse Anti-Human Integrin beta1/CD29 Monoclonal Antibody (Catalog # MAB17781, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog# F0102B). No staining in the Integrin beta1/CD29 knockout A549 cell line was observed. View our protocol for Staining Membrane-associated Proteins.

Detection of Integrin beta1/CD29 by Flow Cytometry

DC-conditioned medium inhibits mesenchymal stromal cells (MSC) differentiation into adipocytes through osteopontin release. (A) MSCs were cultured for 15 days in adipocyte differentiation medium in the presence of 30% DC-CM or RPMI (control condition, ctrl) and stained with Oil Red O to reveal lipid droplets (original magnification 5×) (left panel). Adipocytes were counted in five random fields from one representative well per group (middle panel) and Oil Red O extracted with isopropanol was measured at optical density 490 (right panel) (mean ± SEM of four independent wells). *p < 0.05 vs. ctrl by Student’s t-test. (B) The mRNA levels of ADIPOQ, FABP4, and PPAR gamma2 were analyzed by real-time PCR at days 5 and 12 of culture. Data were shown as means ± SEM (n = 3). *p < 0.05 vs. ctrl by Student’s t-test. (C) MSCs were examined for the expression of CD29 and CD44 by flow cytometry (gray area, isotype control; white area, specific antibody). MSCs were induced by adipogenic differentiation medium in control condition, with rhOPN or DC-CM in the presence or the absence of the indicated antibodies. Relative mRNA expression of ADIPOQ and FABP4 was measured by real-time PCR on day 12 of adipogenic induction. RPL13A was used for normalization. Data were shown as means ± SEM (n = 3). *p < 0.05 vs. ctrl; #p < 0.05 vs DC-CM in presence of the isotype control by one-way ANOVA followed by Tukey’s Multiple Comparison Test. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29910810), licensed under a CC-BY license. Not internally tested by R&D Systems.

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Applications for Human Integrin beta1/CD29 Antibody

Application

Recommended Usage

Blockade of Receptor-ligand Interaction

Mukhopadhyay, N.K. et al. (2004) Ann. Thorac. Surg. 78:450.
Blaschke, F. et al. (2002) Biochem. Biophys. Res. Commun. 296:890.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Human peripheral blood mononuclear cells (PBMCs) and A549 human lung carcinoma cell line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs)

Immunoprecipitation

Lin, Q. et al. (2004) Biochim. Biophys. Acta 1689:175.
Joneckis, C.C. et al. (1993) Blood 82:3548.

Knockout Validated

Integrin beta1/CD29 is specifically detected in A549 lung carcinoma parental cell line but is not detectable in Integrin beta1/CD29 knockout A549 cell line.

Reviewed Applications

Read 5 reviews rated 4.6 using MAB17781 in the following applications:

Tethering reagent to attach non-adherent cells (1 Review)
Western Blot (4 Reviews)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin beta 1/CD29

The Integrin beta1 subunit, also known as CD29, associates with at least ten different Integrin alpha subunits. It regulates not only its multiple ligands, but activates a signaling cascade in its expressing cells. CD29 is proposed to play a role in cell adhesion, apoptosis, and differentiation (1,2). Over amino acids (aa) 21-728, human and mouse share 92% aa sequence identity.

References

Barkan, D. and A.F. Chambers (2011) Clin. Cancer Res. 17:7219.
Humphries, M.J. (2000) Biochem. Soc. Trans. 28:311.

Alternate Names

CD29, ITGB1

Entrez Gene IDs

3688 (Human); 16412 (Mouse); 102144610 (Cynomolgus Monkey)

Gene Symbol

ITGB1

Additional Integrin beta 1/CD29 Products

Product Documents for Human Integrin beta1/CD29 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Human Integrin beta1/CD29 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Integrin beta1/CD29 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB17781

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications