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Human Integrin  beta1/CD29 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB17781P

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FAB17781P
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human, Hamster - Cricetulus (Chinese Hamster), Primate - Chlorocebus aethiops (African Green Monkey)

Applications

Validated:

Knockout Validated, Flow Cytometry

Cited:

Neutralization, Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # P5D2

Product Specifications

Immunogen

Human skin keratinocytes

Specificity

Detects human Integrin beta1/CD29 in flow cytometry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Integrin  beta1/CD29 PE-conjugated Antibody

Detection of Integrin  beta1/CD29 antibody in A549 human lung carcinoma cell line antibody by Flow Cytometry.

Detection of Integrin beta1/CD29 in A549 human lung carcinoma cell line by Flow Cytometry.

A549 human lung carcinoma cell line was stained with Mouse Anti-Human Integrin beta1/CD29 PE-conjugated Monoclonal Antibody (Catalog # FAB17781P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of Integrin  beta1/CD29 antibody in Human PBMCs antibody by Flow Cytometry.

Detection of Integrin beta1/CD29 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human Integrin beta1/CD29 PE-conjugated Monoclonal Antibody (Catalog # FAB17781P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Integrin  beta1/CD29 Antibody Specificity is Shown by Flow Cytometry antibody in Knockout Cell Line.

Integrin  beta1/CD29 Specificity is Shown by Flow Cytometry in Knockout Cell Line.

Integrin beta1/CD29 knockout A549 human lung carcinoma cell line was stained with Mouse Anti-Human Integrin beta1/CD29 PE-conjugated Monoclonal Antibody (Catalog # FAB17781P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). No staining in the Integrin beta1/CD29 knockout A549 cell line was observed. View our protocol for Staining Membrane-associated Proteins.

Applications for Human Integrin  beta1/CD29 PE-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs)

Knockout Validated

Integrin beta1/CD29 is specifically detected in A549 lung carcinoma parental cell line but is not detectable in Integrin beta1/CD29 knockout A549 cell line.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Integrin beta 1/CD29

The Integrin beta1 subunit, also known as CD29, associates with at least ten different Integrin alpha subunits. It regulates not only its multiple ligands, but activates a signaling cascade in its expressing cells. CD29 is proposed to play a role in cell adhesion, apoptosis, and differentiation (1,2). Over amino acids (aa) 21-728, human and mouse share 92% aa sequence identity.

References

  1. Barkan, D. and A.F. Chambers (2011) Clin. Cancer Res. 17:7219.
  2. Humphries, M.J. (2000) Biochem. Soc. Trans. 28:311.

Alternate Names

CD29, ITGB1

Entrez Gene IDs

3688 (Human); 16412 (Mouse); 102144610 (Cynomolgus Monkey)

Gene Symbol

ITGB1

Additional Integrin beta 1/CD29 Products

Product Documents for Human Integrin  beta1/CD29 PE-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Integrin  beta1/CD29 PE-conjugated Antibody

For research use only

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