Human IL-8/CXCL8 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2081

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MAB2081-100
MAB2081-SP
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Key Product Details

Species Reactivity

Human

Applications

ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1028326

View all IL-8/CXCL8 Antibodies »

Product Specifications

Immunogen

E. coli-derived human IL-8/CXCL8
Ser28-Ser99
Accession # P10145.1

Specificity

Detects human IL-8/CXCL8 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human IL-8/CXCL8 Antibody

Human IL-8/CXCL8 ELISA Standard Curve.

Recombinant Human IL-8/CXCL8 protein was serially diluted 2-fold and captured by Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB2081) coated on a Clear Polystyrene Microplate (DY990). Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (MAB2082) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY9998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).
Detection of IL-8/CXCL8 by Immunocytochemistry/ Immunofluorescence

Detection of IL-8/CXCL8 by Immunocytochemistry/ Immunofluorescence

Cell signalingproperties of lipid rafts and LRNVs. (A) PMSCs wereimmunolabeled for caveolin-1 (green) and galectin-1 (top) or beta-catenin(bottom) (red) to visualize colocalization of bioactive signalingproteins within the lipid raft microdomains. Scale bar: 20 μm.(B) Colocalization was further confirmed with co-immunoprecipitationof beta-catenin or galectin-1 with caveolin-1. Mouse-IgG (M-IgG)was used as an antibody control for caveolin-1. (C) HUVECs were treatedwith LRNVs for 48 h and assessed for Akt expression using Westernblotting. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was usedas a loading control. (D) Akt expression was quantified after GAPDHnormalization (n = 3 donor cell banks and repeatedwith three independent experiments). *p < 0.05versus untreated control using a one-sample t-test. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36448709), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human IL-8/CXCL8 Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB2082).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human IL-8/CXCL8 DuoSet ELISA Kit (Catalog # DY208) for convenient development of a sandwich ELISA or the Human IL-8/CXCL8 Quantikine ELISA Kit (Catalog # D8000C) for  a complete optimized ELISA.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-8/CXCL8

Interleukin-8 (IL-8), also known as CXCL8, GCP-1, and NAP-1, is a widely expressed proinflammatory member of the CXC family of chemokines. Near its N-terminus, this 8-9 kDa chemokine contains an ELR motif which is important for its angiogenic properties (1). IL-8/CXCL8 can associate into a homodimer or a heterodimer with CXCL4/PF4 (2), and it can also interact with matrix and cell surface glycosaminoglycans (3). Mature human IL-8/CXCL8 shares 65%-69% amino acid (aa) sequence identiity with canine, feline, and porcine IL-8/CXCL8 (4). There is no IL-8/CXCL8 gene counterpart in rodent. N-terminal truncation by multiple proteases generates a range of shorter forms, and an alternative splice form of human IL-8/CXCL8 carries an eleven aa substitution at the C-terminus (5). The bioactivity of IL-8/CXCL8 is regulated by these truncations, by IL-8/CXCL8 citrullination at Arg5 (N-terminal to the ELR motif) (6), and by the decoy receptor DARC (7). IL-8/CXCL8 effects are mediated through CXCR1/IL-8 RA, which is also used by CXCL6, and through CXCR2/IL-8 RB, which is used by multiple CXC chemokines (1). CXCR1 and CXCR2 associate into functional homodimers and heterodimers with each other (8). Through both CXCR1 and CXCR2, CXCL8 promotes neutrophil adhesion to the vascular endothelium and migration to sites of inflammation (9). It triggers the antimicrobial activation of neutrophils through CXCR1 (10). CXCL8 also binds to Serpin A1/alpha-1 Antitrypsin, and this prevents IL-8/CXCL8 interaction with CXCR1 (11). IL-8/CXCL8 is upregulated in atherosclerotic lesions and other cardiac pathologies where it exacerbates inflammatory tissue damage (12). In addition, it induces VEGF expression, vascular endothelial cell proliferation, angiogenesis, and tumor cell invasiveness (13-16).

References

  1. Lazennec, G. and A. Richmond (2010) Trends Mol. Med. 16:133.
  2. Nesmelova, I.V. et al. (2005) J. Biol. Chem. 280:4948.
  3. Pichert, A. et al. (2012) Biomatter 2:142.
  4. Schmid, J. and C. Weissmann (1987) J. Immunol. 139:250.
  5. Mortier, A. et al. (2008) Pharmacol. Ther. 120:197.
  6. Proost, P. et al. (2008) J. Exp. Med. 205:2085.
  7. Neote, K. et al. (1994) Blood 84:44.
  8. Munoz, L.M. et al. (2009) J. Immunol. 183:7337.
  9. Gerszten, R.E. et al. (1999) Nature 398:718.
  10. Jones, S.A. et al. (1996) Proc. Natl. Acad. Sci. USA 93:6682.
  11. Bergin, D.A. et al. (2010) J. Clin. Invest. 120:4236.
  12. Apostolakis, S. et al. (2009) Cardiovasc. Res. 84:353.
  13. Martin, D. et al. (2009) J. Biol. Chem. 284:6038.
  14. Li, A. et al. (2005) Angiogenesis 8:63.
  15. Waugh, D.J. and C. Wilson (2008) Clin. Cancer Res. 14:6735.
  16. Fernando, R.I. et al. (2011) Cancer Res. 71:5296.

Long Name

Interleukin 8

Alternate Names

CXCL8, GCP1, IL8, LAI, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP1, NCF, TCF, TSG1

Entrez Gene IDs

3576 (Human); 396880 (Porcine); 403850 (Canine); 493836 (Feline)

Gene Symbol

CXCL8

UniProt

P10145.1

Additional IL-8/CXCL8 Products

Product Documents for Human IL-8/CXCL8 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human IL-8/CXCL8 Antibody

For research use only

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