Human IL-4R alpha Antibody

Name: Human IL-4R alpha Antibody
Brand: R&D Systems
SKU: MAB230
Rating: 5 (4 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB230

Citations (23)

(4)

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Key Product Details

Species Reactivity:	Human
Immunogen:	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑4 R Gly24-His232 Accession # P24394
Applications:	Block/Neutralize, CyTOF-ready, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Western Blot
Label:	Unconjugated
Source:	Monoclonal Mouse IgG_2A Clone # 25463
Specificity:	Detects human IL‑4 R alpha in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-9 R, recombinant mouse IL-4 R, rhIL-5 R alpha, rhIL-5 R beta, rhIL-13 R alpha 1, or rhIL-13 R alpha 2 is observed.

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Applications for Human IL-4R alpha Antibody

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Human IL‑4 R alpha (Catalog # 230-4R) under non-reducing conditions only
Flow Cytometry	0.25 µg/10⁶ cells	See below
Immunohistochemistry	8-25 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	5-15 µg/mL	See below

Item	Value
Neutralization	Measured by its ability to neutralize IL‑4-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND₅₀) is typically 3-6 ng/mL in the presence of 0.2 ng/mL Recombinant Human IL‑4.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human IL-4R alpha Antibody

	Flow Cytometry Detection of IL‑4 R alpha in Daudi Human Cell Line by Flow Cytometry. Daudi human Burkitt's lymphoma cell line was stained with Mouse Anti-Human IL-4 Ra Monoclonal Antibody (Catalog # MAB230, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
	Immunohistochemistry IL‑4 R alpha in Human Lymph Node. IL-4 Ra was detected in immersion fixed paraffin-embedded sections of human lymph node using Mouse Anti-Human IL-4 Ra Monoclonal Antibody (Catalog # MAB230) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to plasma cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
	Immunocytochemistry IL‑4 R alpha in HDLM‑2 Human Cell Line. IL-4 Ra was detected in immersion fixed HDLM-2 human Hodgkin's lymphoma cell line using Mouse Anti-Human IL-4 Ra Monoclonal Antibody (Catalog # MAB230) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
	Neutralization Cell Proliferation Induced by IL‑4 and Neutralization by Human IL‑4 R alpha Antibody. Recombinant Human IL‑4 (Catalog # 204-IL) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑4 (0.2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑4 Ra Monoclonal Antibody (Catalog # MAB230). The ND₅₀ is typically 3-6 ng/mL.

Formulation, Preparation and Storage

Purification	Protein A or G purified from ascites
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: IL-4R alpha

Interleukin 4 receptor (IL-4 R) is the transmembrane ligand binding subunit of the heterodimeric IL-4 receptor complex. In the type I receptor complex, IL-4 R associates with the common gamma chain, whereas in the type II receptor complex, it associates with IL-13 R alpha 1. IL-4 R is expressed by B cells, T cells, monocytes and macrophages.

Item	Value
Long Name	Interleukin 4 Receptor alpha
Entrez Gene IDs	3566 (Human); 16190 (Mouse)
Alternate Names	CD124, CD124 antigen, IL-4 R alpha, IL-4 receptor subunit alpha, IL-4R alpha, IL-4R subunit alpha, IL-4R-alpha, IL-4Ra, IL4R, IL4R alpha, IL4RACD124, interleukin 4 receptor, interleukin-4 receptor alpha chain, interleukin-4 receptor subunit alpha

Citations for Human IL-4R alpha Antibody (23)

Showing 1 - 10 of 23 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human IL-4R alpha Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: Neutralization

S Mairpady S et al. (2022), Hyperinflammatory environment drives dysfunctional myeloid cell effector response to bacterial challenge in COVID-19 PloS Pathogens, 18(1):e1010176.
PMID: 35007290
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

F Fang et al. (2022), IL-4 prevents adenosine-mediated immunoregulation by inhibiting CD39 expression JCI Insight, 7(12).
PMID: 35730568
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Q D'Arcy et al. (2022), The IL-4/IL-13 signaling axis promotes prostatic fibrosis PLoS ONE, 17(10):e0275064.
PMID: 36201508
Species: Mouse

Applications: In Vivo

J Karhausen et al. (2020), Platelets trigger perivascular mast cell degranulation to cause inflammatory responses and tissue injury Sci Adv, 6(12):eaay6314.
PMID: 32206714
Species: Human
Sample Types: Whole Tissue
Applications: IHC

P Dolzani et al. (2019), Ex vivo physiological compression of human osteoarthritis cartilage modulates cellular and matrix components PLoS ONE, 14(9):e0222947.
PMID: 31550275
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

M Gaggianesi et al. (2017), IL-4 primes the dynamics of breast cancer progression via DUSP4 inhibition Cancer Res., 0(0).
PMID: 28400477
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization

OT Burton et al. (2017), Allergen-specific IgG antibodies signaling via Fc?RIIb promote food tolerance J. Allergy Clin. Immunol., 0(0).
PMID: 28479335
Species: Human
Sample Types: Whole Cells
Applications: IHC

Volonte A et al. (2014), Cancer-initiating cells from colorectal cancer patients escape from T cell-mediated immunosurveillance in vitro through membrane-bound IL-4. J Immunol, 192(1):523-32.
PMID: 24277698
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Assirelli E et al. (2014), Human osteoarthritic cartilage shows reduced in vivo expression of IL-4, a chondroprotective cytokine that differentially modulates IL-1beta-stimulated production of chemokines and matrix-degrading enzymes in vitro. PLoS ONE, 9(5):e96925.
PMID: 24819779
Biancheri P et al. (2014), Absence of a role for interleukin-13 in inflammatory bowel disease. Eur J Immunol, 44(2):370-85.
PMID: 24338958
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Reece P et al. (2014), IL-4 and IL-13 differentially regulate TLR-induced eosinophil-basophil differentiation of cord blood CD34+ progenitor cells. PLoS ONE, 9(6):e100734.
PMID: 24971469
Species: Human
Sample Types: Whole Cells
Applications: Blocking

Sarangthem et al. (2013), Construction and application of elastin like polypeptide containing IL-4 receptor targeting peptide. PLoS ONE, 8(12):e81891.
PMID: 24339977
Species: Human
Sample Types: Whole Cells
Applications: Binding Assay

Boulaftali et al. (2013), Platelet ITAM signaling is critical for vascular integrity in inflammation. J Clin Invest, 123(2):908-16.
PMID: 23348738
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Amano Y et al. (2011), Hrs Recognizes a Hydrophobic Amino Acid Cluster in Cytokine Receptors during Ubiquitin-independent Endosomal Sorting. J. Biol. Chem., 286(17):15458-72.
PMID: 21362618
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

Thors L et al. (2010), Fatty acid amide hydrolase in prostate cancer: association with disease severity and outcome, CB1 receptor expression and regulation by IL-4. PLoS ONE, 5(8).
PMID: 20808855
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Eyerich K et al. (2009), IL-17 in atopic eczema: linking allergen-specific adaptive and microbial-triggered innate immune response. J. Allergy Clin. Immunol., 123(1):59-66.e4.
PMID: 19056110
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Hu A et al. (2009), Th2 cytokine-induced upregulation of 11beta-hydroxysteroid dehydrogenase-1 facilitates glucocorticoid suppression of proasthmatic airway smooth muscle function. Am. J. Physiol. Lung Cell Mol. Physiol., 296(5):L790-803.
PMID: 19251840
Species: Human
Sample Types: Whole Cells
Applications: ICC

Aoudjehane L et al. (2008), Interleukin-4 induces the activation and collagen production of cultured human intrahepatic fibroblasts via the STAT-6 pathway. Lab. Invest., 88(9):973-85.
PMID: 18626468
Species: Human
Sample Types: Whole Cells
Applications: ICC

Aoudjehane L et al. (2007), Interleukin-4 induces human hepatocyte apoptosis through a Fas-independent pathway. FASEB J., 21(7):1433-44.
PMID: 17283223
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Hurst SM et al. (2002), Secretion of oncostatin M by infiltrating neutrophils: regulation of IL-6 and chemokine expression in human mesothelial cells. J. Immunol., 169(9):5244-51.
PMID: 12391243
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Hirst SJ et al. (2002), Selective induction of eotaxin release by interleukin-13 or interleukin-4 in human airway smooth muscle cells is synergistic with interleukin-1beta and is mediated by the interleukin-4 receptor alpha-chain. Am. J. Respir. Crit. Care Med., 165(8):1161-71.
PMID: 11956062
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

de Lavareille A et al. (2001), Clonal Th2 cells associated with chronic hypereosinophilia: TARC-induced CCR4 down-regulation in vivo. Eur. J. Immunol., 31(4):1037-46.
PMID: 11298328
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Albanesi C et al. (2001), A cytokine-to-chemokine axis between T lymphocytes and keratinocytes can favor Th1 cell accumulation in chronic inflammatory skin diseases. J. Leukoc. Biol., 70(4):617-23.
PMID: 11590199

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Customer Reviews for Human IL-4R alpha Antibody (4)

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Human IL-4R alpha Antibody

Name: Anonymous

Application: CyTOF

Sample Tested: Peripheral blood mononuclear cells (PBMCs)

Species: Human

Verified Customer | Posted 05/04/2022

Gated on CD45+ PBMCs
Human IL-4R alpha Antibody

Name: Anonymous

Application: B/N

Sample Tested: LAD2 human mast cells

Species: Human

Verified Customer | Posted 04/07/2022
Human IL-4R alpha Antibody

Name: Anonymous

Application: WB

Sample Tested: Colon cancer cells

Species: Human

Verified Customer | Posted 08/19/2021
Human IL-4 R alpha Antibody

Name: Anonymous

Application: B/N

Sample Tested: Recombinant protein

Species: Human

Verified Customer | Posted 12/06/2017

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Product Documents for Human IL-4R alpha Antibody

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Human IL-4R alpha Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Complete Your Research

Complete Your Experiment

Applications for Human IL-4R alpha Antibody

Scientific Data Examples for Human IL-4R alpha Antibody

Flow Cytometry

Immunohistochemistry

Immunocytochemistry

Neutralization

Formulation, Preparation and Storage

Reconstitution Calculator

Background: IL-4R alpha

Citations for Human IL-4R alpha Antibody (23)

Customer Reviews for Human IL-4R alpha Antibody (4)

Customer Images

Human IL-4R alpha Antibody

Human IL-4R alpha Antibody

Human IL-4R alpha Antibody

Human IL-4 R alpha Antibody

Product Documents for Human IL-4R alpha Antibody

Product Datasheets

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