Human IL-1 beta/IL-1F2 Fluorescein-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # IC201F

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IC201F
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Intracellular Staining by Flow Cytometry

Cited:

Flow Cytometry

Label

FITC (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 8516

View all IL-1 beta/IL-1F2 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human IL-1 beta/IL-1F2
Ala117-Ser269
Accession # P01584

Specificity

Detects human IL-1 beta/IL-1F2 in Western blots. Shows less than 5% cross-reactivity with recombinant mouse (rm) IL-1 beta/IL-1F2 and recombinant porcine IL-1 beta/IL-1F2 and no cross-reactivity with recombinant rat (rr) IL-1 beta/IL-1F2, rmIL‑1 alpha, recombinant human IL‑1ra, rmIL‑1ra, or rrIL‑1 alpha.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human IL-1 beta/IL-1F2 Fluorescein-conjugated Antibody

Detection of IL-1 beta/IL-1F2 antibody in Human Blood Monocytes antibody by Flow Cytometry.

Detection of IL-1 beta/IL-1F2 in Human Blood Monocytes by Flow Cytometry.

Human peripheral blood monocytes treated with LPS were stained with (A) Mouse Anti-Human IL-1 beta/IL-1F2 Fluorescein-conjugated Monoclonal Antibody (Catalog # IC201F) and Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P). Excess Recombinant Human IL-1 beta/IL-1F2 (Catalog # 201-LB) inhibits IC201F staining (B). Quadrant markers were set based on control antibody staining (Catalog # IC002F). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human IL-1 beta/IL-1F2 Fluorescein-conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood monocytes treated with LPS were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

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Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IL-1 beta/IL-1F2

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2, IL1B), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. The 17 kDa molecular weight mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67%-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta. IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.

Long Name

Interleukin 1 beta

Alternate Names

IL-1b, IL-1F2, IL1 beta, IL1B

Entrez Gene IDs

3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)

Gene Symbol

IL1B

UniProt

P01584

Additional IL-1 beta/IL-1F2 Products

Product Documents for Human IL-1 beta/IL-1F2 Fluorescein-conjugated Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human IL-1 beta/IL-1F2 Fluorescein-conjugated Antibody

For research use only

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