Human IL-17RB Antibody

Name: Human IL-17RB Antibody
Brand: R&D Systems
SKU: MAB1207
Rating: 5 (3 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB1207

Citations (5)

(3)

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Key Product Details

Species Reactivity:	Human
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human IL‑17 RB Arg18-Gly289 Accession # Q9NRM6
Applications:	CyTOF-ready, Flow Cytometry, Western Blot
Label:	Unconjugated
Source:	Monoclonal Mouse IgG_2B Clone # 170220
Specificity:	Detects human IL‑17 RB in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse (rm) IL‑17 RB, recombinant human IL-17 R, or rmIL-17 R is observed.

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Applications for Human IL-17RB Antibody

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Human IL‑17B R Fc Chimera (Catalog # 1207-BR)
Flow Cytometry	2.5 µg/10⁶ cells	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human IL-17RB Antibody

	Flow Cytometry Detection of IL‑17 RB in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human IL-17 RB Monoclonal Antibody (Catalog # MAB1207, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Background: IL-17RB

The interleukin 17 (IL-17) family of cytokines, comprising six members (IL-17, IL-17B through IL-17F), are structurally related proteins with a conserved cysteine-knot structure. These proinflammatory cytokines can induce local cytokine productions and are involved in the regulation of the immune response. The cognate receptors activated by some of these cytokines have been identified (1). Interleukin-17 B Receptor (IL-17 RB), also known as IL-17Rh1, IL-17ER and EVI27, represents the second receptor of the IL-17 family of cytokines to be recognized (2-4). Human IL-17 RB cDNA encodes a 502 amino acid (aa) residue type I membrane protein with a putative 17 aa signal peptide, a 275 aa extracellular domain, a 21 aa transmembrane domain and a 189 aa cytoplasmic tail. By alternative splicing, a secreted variant of IL-17 RB has also been identified (4). Human and mouse IIL-17 RB share 76% aa sequence identity. The human IL-17 RB protein sequence is only 19.2% identical to the human IL-17 R sequence, but the two receptors share many conserved cysteine residues within their extracellular domains as well as additional conserved elements within their cytoplasmic domains. Three additional type I transmembrane receptors with homology to IL-17 R have been reported, increasing the number of the IL-17 R family members to five (5, 6). By Northern blot analysis, human IL-17 RB is highly expressed in kidneys and liver but is expressed at lower levels in testes, brain, small intestine and other endocrine tissues (2-4). The expression of IL-17 RB is significantly up-regulated under inflammatory conditions. IL-17 RB binds strongly to IL-17E and weakly to IL-17B. It does not bind IL-17, IL-17C, and IL-17F. Activation of IL-17 RB by its ligands results in the activation of nuclear factor kappa-B (2-4).

Item	Value
References	Aggarwal, S. and A.L. Gurney (2002) J. Leukoc. Biol. 71:1. Shi Y, et al. (2000) J. Biol. Chem. 275:19167. Lee, J, et al. (2001) J. Biol. Chem. 276:1660. Tian E, et al. (2000) Oncogene 19:2098. Haudenschild, D. et al. (2002) J. Biol. Chem. 277:4309. Hurst, S.D. et al. (2002) J. Immunol. 169:443.
Long Name	Interleukin 17B Receptor
Entrez Gene IDs	55540 (Human); 50905 (Mouse)
Alternate Names	CRL4IL-17 receptor homolog 1, Cytokine receptor-like 4, EVI27IL-17B receptor, Evi27, IL-17 RB, IL-17 RH1, IL-17 receptor B, IL-17ER, IL-17RB, IL-17Rh1, IL17BRinterleukin 17B receptor, IL17RB, IL17RH1MGC5245, IL17Rh1, Interleukin-17B receptor, cytokine receptor CRL4, interleukin 17 receptor B, interleukin 17 receptor homolog 1, interleukin-17 receptor B

Citations for Human IL-17RB Antibody (5)

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Citations are publications that use Bio-Techne products. Selected citations for Human IL-17RB Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Lin Li (2016), IL-17RB(+) granulocytes are associated with airflow obstruction in asthma Ann. Allergy Asthma Immunol, 117(6):674-679.
PMID: 27979026
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

SM Bal et al. (2016), IL-1?, IL-4 and IL-12 control the fate of group 2 innate lymphoid cells in human airway inflammation in the lungs Nat Immunol, 0(0).
PMID: 27111145
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

Hvid M et al. (2011), IL-25 in atopic dermatitis: a possible link between inflammation and skin barrier dysfunction? J. Invest. Dermatol., 131(1):150-7.
PMID: 20861853
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Islam SA et al. (2011), Mouse CCL8, a CCR8 agonist, promotes atopic dermatitis by recruiting IL-5+ T(H)2 cells. Nat. Immunol., 12(2):167-77.
PMID: 21217759
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

LÃƒÂ©tuvÃƒÂ© S et al. (2006), IL-17E upregulates the expression of proinflammatory cytokines in lung fibroblasts. J. Allergy Clin. Immunol., 117(3):590-6.
PMID: 16522458

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Customer Reviews for Human IL-17RB Antibody (3)

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Human IL-17RB Antibody

Name: Amama Kanwal

Application: ICC/IF

Sample Tested: Human Fibrotic Lung

Species: Human

Verified Customer | Posted 05/31/2022
Human IL-17RB Antibody

Name: Teresa Williams

Application: ICC/IF

Sample Tested: lung biopsy

Species: Human

Verified Customer | Posted 12/14/2020
Human IL-17 RB Antibody

Name: Anonymous

Application: Flow

Sample Tested: Peripheral blood cells

Species: Human

Verified Customer | Posted 07/27/2016

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Product Documents for Human IL-17RB Antibody

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Human IL-17RB Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Human IL-17RB Antibody

Scientific Data Examples for Human IL-17RB Antibody

Flow Cytometry

Formulation, Preparation and Storage

Plan Your Experiments

Reconstitution Calculator

Background: IL-17RB

Citations for Human IL-17RB Antibody (5)

Customer Reviews for Human IL-17RB Antibody (3)

Customer Images

Human IL-17RB Antibody

Human IL-17RB Antibody

Human IL-17 RB Antibody

Product Documents for Human IL-17RB Antibody

Product Datasheets

COA

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