Human IL-17RB PE-conjugated Antibody

Name: Human IL-17RB PE-conjugated Antibody
Brand: R&D Systems
SKU: FAB1207P

R&D Systems, Inc. a Bio-Techne Brand

Catalog # FAB1207P

Citations (3)

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Species Reactivity:	Human
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human IL-17 RB Arg18-Gly289 Accession # Q9NRM6
Applications:	Flow Cytometry
Label:	Phycoerythrin (Excitation=488 nm, Emission=565-605 nm)
Source:	Monoclonal Mouse IgG_2B Clone # 170220
Specificity:	Detects human IL-17 RB in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse (rm) IL‑17 RB, recombinant human IL-17 R, or rmIL-17 R.

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Applications for Human IL-17RB PE-conjugated Antibody

	Recommended Concentration	Sample
Flow Cytometry	10 µL/10⁶ cells	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human IL-17RB PE-conjugated Antibody

	Flow Cytometry Detection of IL-17 RB in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human IL-17 RB PE-conjugated Monoclonal Antibody (Catalog # FAB1207P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Formulation	Supplied in a saline solution containing BSA and Sodium Azide.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

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Background: IL-17RB

The interleukin 17 (IL-17) family of cytokines, comprising six members (IL-17, IL-17B through IL-17F), are structurally related proteins with a conserved cysteine-knot structure. These proinflammatory cytokines can induce local cytokine productions and are involved in the regulation of the immune response. The cognate receptors activated by some of these cytokines have been identified (1). Interleukin-17 B Receptor (IL-17B R), also known as IL-17Rh1, IL-17ER and EVI27, represents the second receptor of the IL-17 family of cytokines to be recognized (2-4). Human IL-17B R cDNA encodes a 502 amino acid (aa) residue type I membrane protein with a putative 17 aa signal peptide, a 275 aa extracellular domain, a 21 aa transmembrane domain and a 189 aa cytoplasmic tail. By alternative splicing, a secreted variant of IL-17B R has also been identified (4). Human and mouse IL‑17B R share 76% aa sequence identity. The human IL-17B R protein sequence is only 19.2% identical to the human IL-17 R sequence, but the two receptors share many conserved cysteine residues within their extracellular domains as well as additional conserved elements within their cytoplasmic domains. Three additional type I transmembrane receptors with homology to IL-17 R have been reported, increasing the number of the IL-17 R family members to five (5, 6). By Northern blot analysis, human IL-17B R is highly expressed in kidneys and liver but is expressed at lower levels in testes, brain, small intestine and other endocrine tissues (2-4). The expression of IL-17B R is significantly up-regulated under inflammatory conditions. IL-17 RB binds strongly to IL-17E and weakly to IL-17B. It does not bind IL-17, IL-17C, and IL-17F. Activation of IL-17B R by its ligands results in the activation of nuclear factor kappa-B (2-4).

Item	Value
References	Aggarwal, S. and A.L. Gurney (2002) J. Leukoc. Biol. 71:1. Shi Y, et al. (2000) J. Biol. Chem. 275:19167. Lee, J, et al. (2001) J. Biol. Chem. 276:1660. Tian E, et al. (2000) Oncogene 19:2098. Haudenschild, D. et al. (2002) J. Biol. Chem. 277:4309. Hurst, S.D. et al. (2002) J. Immunol. 169:443.
Long Name	Interleukin 17B Receptor
Entrez Gene IDs	55540 (Human); 50905 (Mouse)
Alternate Names	CRL4IL-17 receptor homolog 1, Cytokine receptor-like 4, EVI27IL-17B receptor, Evi27, IL-17 RB, IL-17 RH1, IL-17 receptor B, IL-17ER, IL-17RB, IL-17Rh1, IL17BRinterleukin 17B receptor, IL17RB, IL17RH1MGC5245, IL17Rh1, Interleukin-17B receptor, cytokine receptor CRL4, interleukin 17 receptor B, interleukin 17 receptor homolog 1, interleukin-17 receptor B

Citations for Human IL-17RB PE-conjugated Antibody (3)

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Citations are publications that use Bio-Techne products. Selected citations for Human IL-17RB PE-conjugated Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

E Ferretti et al. (2018), IL-25 dampens the growth of human germinal center-derived B-cell non Hodgkin Lymphoma by curtailing neoangiogenesis Oncoimmunology, 7(3):e1397249.
PMID: 29399397
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Hvid M et al. (2011), IL-25 in atopic dermatitis: a possible link between inflammation and skin barrier dysfunction? J. Invest. Dermatol., 131(1):150-7.
PMID: 20861853
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Caruso R et al. (2009), Inhibition of monocyte-derived inflammatory cytokines by IL-25 occurs via p38 Map kinase-dependent induction of Socs-3. Blood, 113(15):3512-9.
PMID: 19129540

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Human IL-17RB PE-conjugated Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Human IL-17RB PE-conjugated Antibody

Scientific Data Examples for Human IL-17RB PE-conjugated Antibody

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