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Human IL-17RA/IL-17R Antibody
Catalog # MAB177 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Knockout Validated, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 133617
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Product Summary for Human IL-17RA/IL-17R Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL‑17 RA/IL‑17 R
Leu33-Trp320
Accession # Q96F46
Leu33-Trp320
Accession # Q96F46
Specificity
Detects human IL‑17 RA/IL‑17 R in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) IL‑17 R, rmIL‑17B R, or recombinant human IL‑17B R is observed. In Western blots, no cross-reactivity with rmIL-17 R is observed
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human IL-17RA/IL-17R Antibody
Detection of IL-17 R in K562 Human Cell Line by Flow Cytometry.
K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human IL-17 R Monoclonal Antibody (Catalog # MAB177, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by anti-Mouse IgG APC-conjugated secondary antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
CXCL1/GRO alpha Secretion Induced by IL‑17/IL‑17A and Neutralization by Human IL‑17 RA/IL‑17 R Antibody.
Recombinant Human IL-17/IL-17A induces CXCL1/GROa secretion in the HT-29 human colon adenocarcinoma cell line in a dose-dependent manner (orange line), as measured by the Human CXCL1/GROa DuoSet (Catalog # DY275). CXCL1/GROa secretion elicited by Recombinant Human IL-17/IL-17A (40 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-17 RA/IL-17 R Monoclonal Antibody (Catalog # MAB177). The ND50 is typically 0.4-2 µg/mL.
IL-17 RA/IL-17 R Specificity is Shown by Flow Cytometry in Knockout Cell Line.
IL-17 RA/IL-17 R knockout K562 human myelogenous leukemia cell line was stained with Mouse Anti-Human IL-17 RA/IL-17 R Monoclonal Antibody (Catalog # MAB177, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B). No staining in the IL-17 RA/IL-17 R knockout K562 cell line was observed. View our protocol for Staining Membrane-associated Proteins.Applications for Human IL-17RA/IL-17R Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: K562 human chronic myelogenous leukemia cell line
Sample: K562 human chronic myelogenous leukemia cell line
Knockout Validated
IL-17 RA/IL-17 R is specifically detected in K562 myelogenous leukemia parental cell line but is not detectable in IL-17 RA/IL-17 R knockout K562 cell line.
Western Blot
1 µg/mL
Sample: Recombinant Human IL‑17 RA/IL‑17 R Fc Chimera (Catalog # 177-IR)
Sample: Recombinant Human IL‑17 RA/IL‑17 R Fc Chimera (Catalog # 177-IR)
Neutralization
Measured by its ability to neutralize IL‑17/IL‑17A-induced CXCL1/GRO alpha secretion in the HT‑29 human colon adenocarcinoma cell line. The Neutralization Dose (ND50) is typically 0.4-2 μg/mL in the presence of 40 ng/mL Recombinant Human IL‑17/IL‑17A.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-17RA/IL-17R
Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleotropic cytokine. IL-17 binds to IL-17 receptor (IL-17 R) which shares no homology with any known family of receptors. While the expression of IL-17 is restricted to activated T cells, the IL-17 R mRNA exhibits a broad tissue distribution, and has been detected in virtually all cells and tissues tested. Human IL-17 R is a 120 kDa, 866 amino acid (aa) type I membrane glycoprotein with a 293 aa extracellular domain, a 21 aa carboxy-proximal transmembrane domain, and a 525 aa cytoplasmic tail. Within the ECD, human IL-17 R shares 72% aa sequence identity with mouse and rat IL‑17 R. The signaling events of IL-17 includes activation of NF-kappa B and JNK, and require TNF receptor-associated factors 6 (TRAF6) in the signaling pathway.
References
- Yao, Z. et al. (1997) Cytokine 9:794.
- Schwander, R. et al. (2000) J. Exp. Med. 191:1233.
Long Name
Interleukin 17 Receptor
Alternate Names
CD217, Cdw217, IL-17 R, IL-17 RA, IL17RA
Entrez Gene IDs
Gene Symbol
IL17RA
UniProt
Product Documents for Human IL-17RA/IL-17R Antibody
Product Specific Notices for Human IL-17RA/IL-17R Antibody
For research use only
Citations for Human IL-17RA/IL-17R Antibody (17)
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Citations are publications that use Bio-Techne products. Selected citations for Human IL-17RA/IL-17R Antibody include:
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Species: Human
Sample Types: Whole Cells
Applications: NeutralizationG Margetts et al. (2022-05-12), Evidence for anti-inflammatory effects and modulation of neurotransmitter metabolism by Salvia officinalis L BMC complementary medicine and therapies, 2022-022(1):131.
PMID: 35550086 -
Species: Human
Sample Types: Whole Tissue
Applications: IHCJW Bolt et al. (2022-01-29), Impact of Adalimumab Treatment on Interleukin-17 and Interleukin-17 Receptor Expression in Skin and Synovium of Psoriatic Arthritis Patients with Mild Psoriasis Biomedicines, 2022-010(2).
PMID: 35203534 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotA Gatsiou et al. (2021-07-22), Interleukin-17A Triggers the Release of Platelet-Derived Factors Driving Vascular Endothelial Cells toward a Pro-Angiogenic State Cells, 2021-010(8).
PMID: 34440624 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationPA Szabo et al. (2017-02-20), Rapid and Rigorous IL-17A Production by a Distinct Subpopulation of Effector Memory T Lymphocytes Constitutes a Novel Mechanism of Toxic Shock Syndrome Immunopathology J. Immunol, 2017-00(0).
PMID: 28219889 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationJ Wu et al. (2016-12-08), Autophagy impacts on oxaliplatin-induced hepatocarcinoma apoptosis via the IL-17/IL-17R-JAK2/STAT3 signaling pathway Oncol Lett, 2016-113(2):770-776.
PMID: 28356957 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotSchminke B et al. (2015-12-14), Interleukin 17 inhibits progenitor cells in rheumatoid arthritis cartilage. Eur J Immunol, 2015-146(2):440-5.
PMID: 26558442 -
Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
Sample Types: Whole Cells
Applications: NeutralizationShen H et al. (2015-02-01), Th17-related cytokines contribute to recall-like expansion/effector function of HMBPP-specific Vgamma2Vdelta2 T cells after Mycobacterium tuberculosis infection or vaccination. Eur J Immunol, 2015-045(2):442-51.
PMID: 25141829 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryBellini A et al. (2011-12-21), Interleukin (IL)-4, IL-13, and IL-17A differentially affect the profibrotic and proinflammatory functions of fibrocytes from asthmatic patients. Mucosal Immunol, 2011-15(2):140-9.
PMID: 22189956 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationRamirez-Carrozzi V et al. (2011-10-12), IL-17C regulates the innate immune function of epithelial cells in an autocrine manner. Nat. Immunol., 2011-112(12):1159-66.
PMID: 21993848 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationChang Y et al. (2011-02-22), TH17 cytokines induce human airway smooth muscle cell migration. J. Allergy Clin. Immunol., 2011-0127(4):1046-53.e1-2.
PMID: 21345484 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationCorrigan CJ et al. (2011-01-04), T-helper cell type 2 (Th2) memory T cell-potentiating cytokine IL-25 has the potential to promote angiogenesis in asthma. Proc. Natl. Acad. Sci. U.S.A., 2011-0108(4):1579-84.
PMID: 21205894 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotCattaruzza L et al. (2009-09-01), Functional coexpression of Interleukin (IL)-7 and its receptor (IL-7R) on Hodgkin and Reed-Sternberg cells: Involvement of IL-7 in tumor cell growth and microenvironmental interactions of Hodgkin's lymphoma. Int. J. Cancer, 2009-0125(5):1092-101.
PMID: 19391137 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationShahrara S et al. (2009-03-15), IL-17 induces monocyte migration in rheumatoid arthritis. J. Immunol., 2009-0182(6):3884-91.
PMID: 19265168 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationEid RE et al. (2009-03-02), Interleukin-17 and interferon-gamma are produced concomitantly by human coronary artery-infiltrating T cells and act synergistically on vascular smooth muscle cells. Circulation, 2009-0119(10):1424-32.
PMID: 19255340 -
Species: Human
Sample Types: RNA,Whole Tissue
Applications: Western Blot,IHC-PZrioual S et al. (2008-01-01), IL-17RA and IL-17RC receptors are essential for IL-17A-induced ELR+ CXC chemokine expression in synoviocytes and are overexpressed in rheumatoid blood. J. Immunol., 2008-0180(1):655-63.
PMID: 18097068 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryZola H et al. (2006-12-04), CD molecules 2006--human cell differentiation molecules. J. Immunol. Methods, 2006-1319(1):1-5.
PMID: 17174972 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryNumasaki M et al. (2005-11-01), IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis. J. Immunol., 2005-1175(9):6177-89.
PMID: 16237115
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