Human IL-17RA/IL-17R PE-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB177P
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Flow Cytometry
Cited:
Western Blot, Flow Cytometry
Label
Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 133617
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL-17 R
Leu33-Trp320
Accession # Q96F46
Leu33-Trp320
Accession # Q96F46
Specificity
Detects human IL-17 R in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) IL‑17 R, rmIL‑17B R, or recombinant human IL‑17B R is observed. In Western blots, no cross-reactivity with rmIL-17 R is observed
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human IL-17RA/IL-17R PE-conjugated Antibody
Detection of IL-17 R in K562 Human Cell Line by Flow Cytometry.
K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human IL-17 R PE-conjugated Monoclonal Antibody (Catalog # FAB177P, filled histogram) or isotype control antibody (IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of IL-17RA/IL-17R by Western Blot
Evaluation of IL-17 signaling in TWIST1-knockdown BC cells compared to control cells. (A) Protein levels of Act1 and IL-17RA in ShCTRL and ShTWIST cells. GAPDH was used to ensure that the same sample amounts were loaded for immunoblotting. Densitometric analysis was done using ImageJ software. (B) Levels of secreted IL-6 and IL-8 proteins in the supernatant of ShCTRL and ShTWIST cells. Data are shown as the mean (±SD). * p < 0.05. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34830027), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human IL-17RA/IL-17R PE-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: K562 human chronic myelogenous leukemia cell line
Sample: K562 human chronic myelogenous leukemia cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-17RA/IL-17R
Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleotropic cytokine. IL-17 binds to IL-17 receptor (IL-17 R) which shares no homology with any known family of receptors. While the expression of IL-17 is restricted to activated T cells, the IL-17 R mRNA exhibits a broad tissue distribution, and has been detected in virtually all cells and tissues tested. Human IL-17 R is a 120 kDa, 866 amino acid (aa) type I membrane glycoprotein with a 293 aa extracellular domain, a 21 aa carboxy-proximal transmembrane domain, and a 525 aa cytoplasmic tail. Within the ECD, human IL-17 R shares 72% aa sequence identity with mouse and rat IL-17 R. The signaling events of IL-17 includes activation of NF-kappa B and JNK, and require TNF receptor-associated factors 6 (TRAF6) in the signaling pathway.
References
- Yao, Z. et al. (1997) Cytokine 9:794.
- Schwander, R. et al. (2000) J. Exp. Med. 191:1233.
Long Name
Interleukin 17 Receptor
Alternate Names
CD217, Cdw217, IL-17 R, IL-17 RA, IL17RA
Entrez Gene IDs
Gene Symbol
IL17RA
UniProt
Additional IL-17RA/IL-17R Products
Product Documents for Human IL-17RA/IL-17R PE-conjugated Antibody
Product Specific Notices for Human IL-17RA/IL-17R PE-conjugated Antibody
For research use only
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