Daudi cells were stained with Mouse Anti-Human IL-12 R beta1 Monoclonal Antibody (Catalog # MAB839, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human IL-12 R beta1 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Human peripheral blood mononuclear cells treated with PHA; Daudi human Burkitt's lymphoma cell line

Reviewed Applications

Read 1 review rated 4 using MAB839 in the following applications:

ELISA (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-12 R beta 1

Interleukin12 (IL-12) is a key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells. The biological activities of IL-12 are mediated through the high-affinity receptor complex composed of two subunits designated IL-12 R beta1 and IL-12 R beta2. Individually, IL-12 R beta1 and IL-12 R beta2 bind IL‑12 with low affinity. Co-expression of both subunits confers high-affinity binding and is required for IL-12 activity. Both IL-12 receptor subunits are type I transmembrane proteins that share similarities with the gp130/G-CSF R subgroup in the cytokine receptor superfamily. IL-12 R beta1 cDNA encodes a 662 amino acid (aa) protein with a putative 23 aa signal peptide that is cleaved to generate the mature protein with a 522 aa extracellular domain, a 25 aa transmembrane domain and a 92 aa cytoplasmic region. Expression of IL-12 R beta1 is detected in activated T cells, NK cells and B cells. The expression of IL‑12 R beta2 is more restricted and appears to be limited to Th2 cells.

References

Gately, M.K. et al. (1998) Annu. Rev. Immunol. 16:495.

Long Name

Interleukin 12 Receptor beta 1

Alternate Names

CD212, IL-12Rb1, IL12R beta 1, IL12RB1

Entrez Gene IDs

3594 (Human); 16161 (Mouse)

Gene Symbol

IL12RB1

UniProt

P42701

Additional IL-12 R beta 1 Products

Product Documents for Human IL-12 R beta1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human IL-12 R beta1 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human IL-12 R beta1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB839

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all IL-12 R beta 1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human IL-12 R beta1 Antibody

Detection of IL-12 R beta1 in Daudi cells by Flow Cytometry