Human IL-12 R beta1 APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB839A
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 69310
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL-12 R beta1
Cys24-Glu540
Accession # P42701
Cys24-Glu540
Accession # P42701
Specificity
Detects human IL-12 R beta1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse IL-12 R beta1 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human IL-12 R beta1 APC-conjugated Antibody
Detection of IL-12 R beta1 in Daudi cells by Flow Cytometry.
Daudi cells were stained with Mouse Anti-Human IL-12 R beta1 APC-conjugated Monoclonal Antibody (Catalog # FAB839A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of IL-12 R beta1 in PBMC cells by Flow Cytometry.
PBMC cells treated with 5ug/ml PHA (Sigma) for 48-72 hours were stained with Mouse Anti-Human CD3 epsilon PE-conjugated Monoclonal Antibody (Catalog # FAB100P) and either (A) Mouse Anti-Human IL-12 R beta1 APC-conjugated Monoclonal Antibody (Catalog # FAB839A) or (B) Mouse IgG1 Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.Applications for Human IL-12 R beta1 APC-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: PBMC treated with 5ug/ml PHA (Sigma) for 48-72 hours and Daudi cells.
Sample: PBMC treated with 5ug/ml PHA (Sigma) for 48-72 hours and Daudi cells.
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-12 R beta 1
Interleukin12 (IL-12) is a key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells. The biological activities of IL-12 are mediated through the high-affinity receptor complex composed of two subunits designated IL-12 R beta1 and IL-12 R beta2. Individually, IL-12 R beta1 and IL-12 R beta2 bind IL‑12 with low affinity. Co-expression of both subunits confers high-affinity binding and is required for IL-12 activity. Both IL-12 receptor subunits are type I transmembrane proteins that share similarities with the gp130/G-CSF R subgroup in the cytokine receptor superfamily. IL-12 R beta1 cDNA encodes a 662 amino acid (aa) protein with a putative 23 aa signal peptide that is cleaved to generate the mature protein with a 522 aa extracellular domain, a 25 aa transmembrane domain and a 92 aa cytoplasmic region. Expression of IL-12 R beta1 is detected in activated T cells, NK cells and B cells. The expression of IL‑12 R beta2 is more restricted and appears to be limited to Th2 cells.
References
- Gately, M.K. et al. (1998) Annu. Rev. Immunol. 16:495.
Long Name
Interleukin 12 Receptor beta 1
Alternate Names
CD212, IL-12Rb1, IL12R beta 1, IL12RB1
Gene Symbol
IL12RB1
UniProt
Additional IL-12 R beta 1 Products
Product Documents for Human IL-12 R beta1 APC-conjugated Antibody
Product Specific Notices for Human IL-12 R beta1 APC-conjugated Antibody
For research use only
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