Human ICOS Antibody

Name: Human ICOS Antibody
Brand: R&D Systems
SKU: MAB6975

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB6975

Citations (1)

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Key Product Details

Species Reactivity:	Human
Immunogen:	NS0 mouse myeloma cell line transfected with human ICOS and mouse myeloma cell line NS0-derived recombinant human ICOS cocktail Glu21-Phe141 Accession # Q9Y6W8
Applications:	CyTOF-ready, Flow Cytometry
Label:	Unconjugated
Source:	Monoclonal Mouse IgG₁ Clone # 669222
Specificity:	Detects human ICOS in direct ELISAs.

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Applications for Human ICOS Antibody

	Recommended Concentration	Sample
Flow Cytometry	2.5 µg/10⁶ cells	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human ICOS Antibody

	Flow Cytometry Detection of ICOS in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with 50 ng/mL PMA and 500 ng/mL calcium ionomycin for 24 hours were stained with Mouse Anti-Human ICOS Monoclonal Antibody (Catalog # MAB6975) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Mouse Anti-Human CD3e PE-conjugated Monoclonal Antibody (Catalog # FAB100P). Quadrant markers were set based on control antibody staining (Catalog # MAB002).

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Reconstitution	Sterile PBS to a final concentration of 0.5 mg/mL. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Background: ICOS

Inducible costimulator (ICOS), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the growing CD28 family of immune costimulatory receptors. Other family members are CD28, CTLA-4, and PD-1. Human ICOS is a homodimeric type I transmembrane protein consisting of 199 amino acids (aa) with a putative 20 aa signal sequence, a 121 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain. ICOS shares approximately 39% amino acid similarity with CD28 and CTLA-4. Human and mouse ICOS share approximately 72% amino acid identity. ICOS is expressed on most CD45RO⁺ cells. ICOS expression is up-regulated within approximately 24-48 hours of activation on T_h primed cells. B7-H2, a member of the B7 family of costimulatory ligands, has been identified as the ICOS ligand. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and T_h differentiation.

Item	Value
References	Aicher, A. et al. (2000) J. Immunol. 164:4689. Coyle, A.J. et al. (2000) Immunity 13:95. Coyle, A.J. and J.C. Gutierrez-Ramos (2001) Nat. Immunol. 2:203. Gonzalo, J.A. et al. (2001) J. Immunol. 166:1. Hutloff, A. et al. (1999) Nature 397:263. Mages, H.W. et al. (2000) Eur. J. Immunol. 30:1040. Yoshinaga, S.K. et al. (1999) Nature 402:827.
Long Name	Inducible Co-stimulator
Entrez Gene IDs	29851 (Human); 54167 (Mouse); 102116512 (Cynomolgus Monkey)
Alternate Names	AILIM, AILIMCD278, Activation-inducible lymphocyte immunomediatory molecule, CD278, CD278 antigen, CRP-1, CVID1, ICOS, MGC39850, inducible T-cell co-stimulator, inducible T-cell costimulator, inducible costimulator

Citations for Human ICOS Antibody (1)

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Citations are publications that use Bio-Techne products. Selected citations for Human ICOS Antibody include:

Species: Human
Sample Types: Serum
Applications: ELISA Development

K McKinski et al. (2022), Orthogonal quantification of soluble inducible T-cell costimulator (ICOS) in healthy and diseased human serum Journal of pharmaceutical analysis, 12(2):317-323.
PMID: 35582393

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