Human ICOS Alexa Fluor® 488-conjugated Antibody

Name: Human ICOS Alexa Fluor® 488-conjugated Antibody
Brand: R&D Systems
SKU: FAB6975G

R&D Systems, Inc. a Bio-Techne Brand

Catalog # FAB6975G

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Key Product Details

Species Reactivity:	Human
Immunogen:	NS0 mouse myeloma cell line transfected with human ICOS and mouse myeloma cell line NS0-derived recombinant human ICOS cocktail Glu21-Phe141 Accession # Q9Y6W8
Applications:	Flow Cytometry
Label:	Alexa Fluor 488 (Excitation=488 nm, Emission=515-545 nm)
Specificity:	Detects human ICOS in direct ELISAs.

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Applications for Human ICOS Alexa Fluor® 488-conjugated Antibody

	Recommended Concentration	Sample
Flow Cytometry	0.25-1 µg/10⁶ cells	Human peripheral blood mononuclear cells (PBMCs) treated with PMA and calcium ionomycin

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Formulation	Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

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Background: ICOS

Inducible costimulator (ICOS), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the growing CD28 family of immune costimulatory receptors. Other family members are CD28, CTLA-4, and PD-1. Human ICOS is a homodimeric type I transmembrane protein consisting of 199 amino acids (aa) with a putative 20 aa signal sequence, a 121 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain. ICOS shares approximately 39% amino acid similarity with CD28 and CTLA-4. Human and mouse ICOS share approximately 72% amino acid identity. ICOS is expressed on most CD45RO⁺ cells. ICOS expression is up-regulated within approximately 24-48 hours of activation on T_h primed cells. B7-H2, a member of the B7 family of costimulatory ligands, has been identified as the ICOS ligand. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and T_h differentiation.

Item	Value
References	Aicher, A. et al. (2000) J. Immunol. 164:4689. Coyle, A.J. et al. (2000) Immunity 13:95. Coyle, A.J. and J.C. Gutierrez-Ramos (2001) Nat. Immunol. 2:203. Gonzalo, J.A. et al. (2001) J. Immunol. 166:1. Hutloff, A. et al. (1999) Nature 397:263. Mages, H.W. et al. (2000) Eur. J. Immunol. 30:1040. Yoshinaga, S.K. et al. (1999) Nature 402:827.
Long Name	Inducible Co-stimulator
Entrez Gene IDs	29851 (Human); 54167 (Mouse); 102116512 (Cynomolgus Monkey)
Alternate Names	AILIM, AILIMCD278, Activation-inducible lymphocyte immunomediatory molecule, CD278, CD278 antigen, CRP-1, CVID1, ICOS, MGC39850, inducible T-cell co-stimulator, inducible T-cell costimulator, inducible costimulator

Product Specific Notices for Human ICOS Alexa Fluor® 488-conjugated Antibody

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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