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Human HVEM/TNFRSF14 Antibody

Bio-Techne includes R&D Systems | Catalog # MAB3561

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MAB3561
MAB3561-SP

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 94804

Product Summary for Human HVEM/TNFRSF14 Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant human HVEM/TNFRSF14
Pro37-Val202 (predicted)
Accession # Q92956.3

Specificity

Detects human HVEM/TNFRSF14 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human(rh) 4‑1BB, rhBAFF R, rhDR3, rhDR6, rhCD27, rhCD30, rhCD40, rhEDAR, rhFas, rhGITR, rhLT beta R, rhNGF R, rhOPG, recombinant mouse OX40, rhRANK, rhTAJ, rhTNF RI or rhTNF R2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human HVEM/TNFRSF14 Antibody

HVEM/TNFRSF14 antibody in Human Lung by Immunohistochemistry (IHC-P).

HVEM/TNFRSF14 in Human Lung.

Herpesvirus Entry Mediator (HVEM/TNFRSF14) was detected in immersion fixed paraffin-embedded sections of human lung using Mouse Anti-Human HVEM/TNFRSF14 Monoclonal Antibody (Catalog # MAB3561) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human HVEM/TNFRSF14 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lung
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 2 publications using MAB3561 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HVEM/TNFRSF14

Herpesvirus entry mediator (HVEM), also referred to as TR2 (TNF receptor-like molecule) and ATAR (another TRAF-associated receptor), is a type I membrane protein belonging to the TNF/NGF receptor superfamily. In the TNF superfamily nomenclature, HVEM is referred to as TNFRSF14. Human HVEM cDNA encodes a 283 amino acid (aa) residue protein with a probable 36 aa residue signal peptide, a 166 aa residue extracellular domain, a 23 aa residue transmembrane region and a 58 aa residue cytoplasmic region. The extracellular domain of HVEM contains several cysteine-rich repeats characteristic of TNF receptor superfamily members. The short cytoplasmic region lacks a death domain present in some TNF receptor family members, but contains a TRAF (TNF receptor-associated factor) interaction domain. HVEM expression has been detected in peripheral blood T cells, B cells, monocytes and in various tissues enriched in lymphoid cells. The extracellular domain of HVEM has been shown to interact directly with the herpes simplex virus envelope glycoprotein D. Two TNF superfamily ligands, including the secreted TNF‑ beta (lymphotoxin  alpha) and the membrane protein LIGHT (lymphotoxins, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes), have been shown to be the cellular ligands for HVEM. Besides HVEM, LIGHT can also interact with LT betaR, the receptor for lymphotoxin alpha beta heterotrimer. The role of the HVEM-LIGHT/LT beta receptor-ligand pair in immune function and herpesvirus pathobiology remains to be elucidated.

References

  1. Hsu, H. et al. (1997) J. Biol. Chem. 272:13471.
  2. Mauri, D.N. et al. (1998) Immunity 8:21.
  3. Montgomery, R.I. et al. (1996) Cell 87:427.

Long Name

Herpesvirus Entry Mediator

Alternate Names

ATAR, CD270, LIGHTR, TNFRSF14

Entrez Gene IDs

8764 (Human); 230979 (Mouse); 102137807 (Cynomolgus Monkey)

Gene Symbol

TNFRSF14

UniProt

Additional HVEM/TNFRSF14 Products

Product Documents for Human HVEM/TNFRSF14 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human HVEM/TNFRSF14 Antibody

For research use only

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