Human HVEM/TNFRSF14 Antibody

Name: Human HVEM/TNFRSF14 Antibody
Brand: R&D Systems
SKU: MAB3562

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB3562

Product Datasheet / COA / SDS

Recombinant Monoclonal Antibody.

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Species Reactivity:	Human
Immunogen:	Human embryonic kidney cell HEK293-derived human HVEM/TNFRSF14 Pro37-Val202 Accession # Q92956
Applications:	Immunohistochemistry
Label:	Unconjugated
Source:	Recombinant Monoclonal Rabbit IgG Clone # 2742C
Specificity:	Detects human HVEM/TNFRSF14 in direct ELISAs.

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Applications for Human HVEM/TNFRSF14 Antibody

	Recommended Concentration	Sample
Immunohistochemistry	3-25 µg/mL	Immersion fixed paraffin-embedded sections of human appendix

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human HVEM/TNFRSF14 Antibody

	Immunohistochemistry HVEM/TNFRSF14 in Human Appendix. HVEM/TNFRSF14 was detected in immersion fixed paraffin-embedded sections of human appendix using Rabbit Anti-Human HVEM/TNFRSF14 Monoclonal Antibody (Catalog # MAB3562) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Formulation, Preparation and Storage

Purification	Protein A or G purified from cell culture supernatant
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: HVEM/TNFRSF14

Herpesvirus entry mediator (HVEM), also referred to as TR2 (TNF receptor-like molecule) and ATAR (another TRAF-associated receptor), is a type I membrane protein belonging to the TNF/NGF receptor superfamily. In the TNF superfamily nomenclature, HVEM is referred to as TNFRSF14. Human HVEM cDNA encodes a 283 amino acid (aa) protein with a probable 36 aa signal peptide, a 166 aa extracellular domain, a 23 aa transmembrane region and a 58 aa cytoplasmic region. The extracellular domain of HVEM contains several cysteine-rich repeats characteristic of TNF receptor superfamily members. The short cytoplasmic region lacks a death domain present in some TNF receptor family members, but contains a TRAF (TNF receptor-associated factor) interaction domain. HVEM expression has been detected in peripheral blood T cells, B cells, monocytes and in various tissues enriched in lymphoid cells. The extracellular domain of HVEM has been shown to interact directly with the herpes simplex virus envelope glycoprotein D. Two TNF superfamily ligands, including the secreted TNF‑ beta (lymphotoxin alpha ) and the membrane protein LIGHT (lymphotoxins, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes), have been shown to be the cellular ligands for HVEM. Besides HVEM, LIGHT can also interact with LT beta R, the receptor for lymphotoxin alpha beta heterotrimer.

Item	Value
References	Hsu, H. et al. (1997) J. Biol. Chem. 272:13471. Mauri, D.N. et al. (1998) Immunity 8:21. Montgomery, R.I. et al. (1996) Cell 87:427.
Long Name	Herpesvirus Entry Mediator
Entrez Gene IDs	8764 (Human); 230979 (Mouse); 102137807 (Cynomolgus Monkey)
Alternate Names	ATAR, CD270, CD270 antigen, CD40-like protein, HVEM, HVEMTR2HVEAATAR, Herpes virus entry mediator A, Herpesvirus entry mediator A, HveA, LIGHTR, TNFRSF14, Tumor necrosis factor receptor-like 2, tumor necrosis factor receptor superfamily member 14, tumor necrosis factor receptor superfamily, member 14 (herpesvirus entrymediator), tumor necrosis factor receptor-like gene2

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Human HVEM/TNFRSF14 Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Human HVEM/TNFRSF14 Antibody

Scientific Data Examples for Human HVEM/TNFRSF14 Antibody

Immunohistochemistry

Formulation, Preparation and Storage

Reconstitution Calculator

Background: HVEM/TNFRSF14

Citations for Human HVEM/TNFRSF14 Antibody

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