Human HVEM/TNFRSF14 Antibody

Name: Human HVEM/TNFRSF14 Antibody
Brand: R&D Systems
SKU: MAB3563

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB3563

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Recombinant Monoclonal Antibody.

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Species Reactivity:	Human
Immunogen:	Human embryonic kidney cell HEK293-derived human HVEM/TNFRSF14 Pro37-Val202 Accession # Q92956
Applications:	Block/Neutralize, Flow Cytometry
Label:	Unconjugated
Source:	Recombinant Monoclonal Rabbit IgG Clone # 2742B
Specificity:	Detects human HVEM/TNFRSF14 in ELISA.

View Full Technical Details for Human HVEM/TNFRSF14 Antibody

Applications for Human HVEM/TNFRSF14 Antibody

	Recommended Concentration	Sample
Flow Cytometry	0.25 µg/10⁶ cells	Human PBMCs

Item	Value
Neutralization	In a functional ELISA binding assay, 0.075-0.45 ug/mL of this antibody will block 50% of the binding of 1 ug/mL of recombinant human BTLA His-tag protein (catalog# 9235-BT) to immobilized recombinant human HVEM Fc Chimera coat 1 ug/mL (100 µL/well). At 1 ug/mL, this antibody will block >90% of the binding.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human HVEM/TNFRSF14 Antibody

	Flow Cytometry Detection of HVEM/TNFRSF14 in Human PBMC B Cells by Flow Cytometry. Human PBMC lymphocytes were stained with (A) Rabbit Anti-Human HVEM/TNFRSF14 Monoclonal Antibody (Catalog # MAB3563) or (B) isotype control antibody (MAB1050), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (F0111) and Mouse Anti-Human PE-conjugated CD19 (FAB4867P). Cells were gated on Mouse Anti-Human Alexa Fluor® 405-conjugated CD14 (FAB3832V), Mouse Anti-Human Alexa Fluor® 488-conjugated CD3 (FAB100G), and Mouse Anti-Human Alexa Fluor® 700-conjugated CD56 (FAB24086N) negative populations. Staining was performed using our Staining Membrane-associated Proteins protocol.
	Flow Cytometry Detection of HVEM/TNFRSF14 in Human PBMC T cells by Flow Cytometry. Human PBMC lymphocytes were stained with (A) Rabbit Anti-Human HVEM/TNFRSF14 Monoclonal Antibody (Catalog # MAB3563) or (B) isotype control antibody (MAB1050), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (F0111) and Mouse Anti-Human Alexa Fluor® 488-conjugated CD3 (FAB100G). Cells were gated on Mouse Anti-Human Alexa Fluor® 405-conjugated CD14 (FAB3832V), Mouse Anti-Human PE-conjugated CD19 (FAB4867P), and Mouse Anti-Human Alexa Fluor® 700-conjugated CD56 (FAB24086N) negative populations. Staining was performed using our Staining Membrane-associated Proteins protocol.
	Flow Cytometry Detection of HVEM/TNFRSF14 in Human PBMC NK cells by Flow Cytometry Human PBMC lymphocytes were stained with (A) Rabbit Anti-Human HVEM/TNFRSF14 Monoclonal Antibody (Catalog # MAB3563) or (B) isotype control antibody (MAB1050), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (F0111) and Mouse Anti-Human Alexa Fluor® 700-conjugated CD56 (FAB24086N). Cells were gated on Mouse Anti-Human Alexa Fluor® 405-conjugated CD14 (FAB3832V), Mouse Anti-Human PE-conjugated CD19 (FAB4867P), and Mouse Anti-Human Alexa Fluor® 488-conjugated CD3 (FAB100G) negative populations. Staining was performed using our Staining Membrane-associated Proteins protocol.
	Flow Cytometry Detection of HVEM/TNFRSF14 in Human PBMC Monocytes by Flow Cytometry Human PBMC monocytes were stained with (A) Rabbit Anti-Human HVEM/TNFRSF14 Monoclonal Antibody (Catalog # MAB3563) or (B) isotype control antibody (MAB1050), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (F0111) and Mouse Anti-Human Alexa Fluor® 405-conjugated CD14 (FAB3832V). Cells were gated on Mouse Anti-Human Alexa Fluor® 488-conjugated CD3 (FAB100G), Mouse Anti-Human PE-conjugated CD19 (FAB4867P), and Mouse Anti-Human Alexa Fluor® 700-conjugated CD56 (FAB24086N) negative populations. Staining was performed using our Staining Membrane-associated Proteins protocol.

Formulation, Preparation and Storage

Purification	Protein A or G purified from cell culture supernatant
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: HVEM/TNFRSF14

HVEM (herpesvirus entry mediator), also known as TNFRSF14 and CD270, is a type I membrane protein in the TNF receptor superfamily, and it can both promote and inhibit T cell activity (1). Mature human HVEM consists of a 164 amino acid (aa) extracellular domain (ECD) with three cysteine-rich domains (CRD), a 21 aa transmembrane segment, and a 60 aa cytoplasmic tail with a TRAF interaction domain (2, 3). Within the ECD, human HVEM shares 55% aa sequence identity with mouse and rat HVEM. Alternative splicing generates an additional isoform with a substitution of the N-terminal 10 amino acids including the signal peptide. HVEM is highly expressed on naïve CD4⁺ T cells, CD8⁺ T memory cells, regulatory T cells, dendritic cells, monocytes, and neutrophils (4-8). Its expression declines during effector T cell activation but is up-regulated during Treg activation (4, 5). HVEM functions as a receptor for BTLA, CD160, LIGHT/TNFSF14, and Lymphotoxin-alpha (4, 9‑12). Ligation of HVEM by LIGHT triggers T cell, monocyte, and neutrophil activation (8, 10) and contributes to Th1 inflammation and cardiac allograft rejection (13, 14). In contrast, HVEM binding to CD160 or BTLA suppresses T cell and dendritic cell activation (4, 7, 9, 10) and dampens intestinal inflammation (15). HVEM enhances the development of CD8⁺ T cell memory and Treg function (5, 6). It is additionally expressed on intestinal epithelial cells, where its binding by intraepithelial lymphocyte (IEL) expressed CD160 promotes epitheilal integrity and host defense (16). The herpesvirus envelope glycoprotein gD, which binds HVEM to initiate membrane fusion, can antagonize both BTLA and LIGHT binding (2, 9, 11).

Item	Value
References	del Rio, M.L. et al. (2010) J. Leukoc. Biol. 87:223. Montgomery, R.I. et al. (1996) Cell 87:427. Hsu, H. et al. (1997) J. Biol. Chem. 272:13471. Sedy, J. R. et al. (2005) Nat. Immunol. 6:90. Tao, R. et al. (2008) J. Immunol. 180:6649. Steinberg, M.W. et al. (2013) PLoS One 8:e77992. de Trez, C. et al. (2008) J. Immunol. 180:238. Heo, S.K. et al. (2006) J. Leukoc. Biol. 79:330. Gonzalez, L.C. et al. (2005) Proc. Natl. Acad Sci. USA 102:1116. Cai, G. et al. (2008) Nat. Immunol. 9:176. Mauri, D.N. et al. (1998) Immunity 8:21. Harrop, J.A. et al. (1998) J. Biol. Chem. 273:27548. Wang, J. et al. (2005) J. Immunol. 174:8173. Ye, Q. et al. (2002) J. Exp. Med. 195:795. Steinberg, M.W. et al. (2008) J. Exp. Med. 205:1463. Shui, J.W. et al. (2012) Nature 488:222.
Long Name	Herpesvirus Entry Mediator
Entrez Gene IDs	8764 (Human); 230979 (Mouse); 102137807 (Cynomolgus Monkey)
Alternate Names	ATAR, CD270, CD270 antigen, CD40-like protein, HVEM, HVEMTR2HVEAATAR, Herpes virus entry mediator A, Herpesvirus entry mediator A, HveA, LIGHTR, TNFRSF14, Tumor necrosis factor receptor-like 2, tumor necrosis factor receptor superfamily member 14, tumor necrosis factor receptor superfamily, member 14 (herpesvirus entrymediator), tumor necrosis factor receptor-like gene2

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Human HVEM/TNFRSF14 Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Background: HVEM/TNFRSF14

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