Human gp130 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB628
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
ELISA Capture (Matched Antibody Pair), Immunohistochemistry, Neutralization
Cited:
Immunohistochemistry-Frozen, Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 28105
Product Specifications
Immunogen
Sf 21-derived recombinant human gp130 extracellular domain
Leu24-Glu619 (Glu619-Asp, predicted)
Accession # P40189
Leu24-Glu619 (Glu619-Asp, predicted)
Accession # P40189
Specificity
Detects human gp130 in ELISAs. In sandwich immunoassays, no significant cross-reactivity or interference with recombinant human (rh) CNTF, rhIL-6, rhIL-6 R, recombinant mouse (rm) IL-6, rhIL-11, rhLIF, rhLIF R, rmLIF, or rhOSM is observed. This antibody blocks the human gp130-mediated bioactivities induced by IL-6, IL-11, LIF, OSM, and CNTF.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human gp130 Antibody
gp130 in Human Pancreas Tissue.
gp130 was detected in immersion fixed paraffin-embedded sections of human pancreas tissue using Mouse Anti-Human gp130 Monoclonal Antibody (Catalog # MAB628) at 5 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (brown; Catalog # VC001) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in exocrine cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Cell Proliferation Induced by Oncostatin M/OSM and Neutralization by Human gp130 Antibody.
Recombinant Human Oncostatin M/OSM stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human Oncostatin M/OSM (0.8 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human gp130 Monoclonal Antibody (Catalog # MAB628). The ND50 is typically 0.02-0.1 µg/mL.Applications for Human gp130 Antibody
Application
Recommended Usage
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreas tissue
Sample: Immersion fixed paraffin-embedded sections of human pancreas tissue
Neutralization
Measured by its ability to neutralize Oncostatin M/OSM-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.02-0.1 µg/mL in the presence of 0.8 ng/mL Recombinant Human Oncostatin M/OSM.
Human gp130 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 4 reviews rated 4 using MAB628 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: gp130
Long Name
Glycoprotein 130
Alternate Names
CD130, IL6ST
Gene Symbol
IL6ST
UniProt
Additional gp130 Products
Product Documents for Human gp130 Antibody
Product Specific Notices for Human gp130 Antibody
For research use only
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