Human gp130 Antibody

Name: Human gp130 Antibody
Brand: R&D Systems
SKU: MAB628
Rating: 4 (4 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB628

Citations (1)

(4)

Product Datasheet / COA / SDS

Catalog #

Availability

Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Species Reactivity:	Human
Immunogen:	Sf 21-derived recombinant human gp130 extracellular domain Leu24-Glu619 (Glu619-Asp, predicted) Accession # P40189
Applications:	Block/Neutralize, ELISA Capture, Immunohistochemistry
Label:	Unconjugated
Source:	Monoclonal Mouse IgG₁ Clone # 28105
Specificity:	Detects human gp130 in ELISAs. In sandwich immunoassays, no significant cross-reactivity or interference with recombinant human (rh) CNTF, rhIL-6, rhIL-6 R, recombinant mouse (rm) IL-6, rhIL-11, rhLIF, rhLIF R, rmLIF, or rhOSM is observed. This antibody blocks the human gp130-mediated bioactivities induced by IL-6, IL-11, LIF, OSM, and CNTF.

View Full Technical Details for Human gp130 Antibody

Applications for Human gp130 Antibody

	Recommended Concentration	Sample
Immunohistochemistry	5-25 µg/mL	See below

Human gp130 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human gp130 Biotinylated Antibody (Catalog # BAF228)

Standard: Recombinant Human gp130 Protein (Catalog # 228-GP)

Item	Value
Neutralization	Measured by its ability to neutralize Oncostatin M/OSM-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND₅₀) is typically 0.02-0.1 µg/mL in the presence of 0.8 ng/mL Recombinant Human Oncostatin M/OSM.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human gp130 Antibody

	Immunohistochemistry gp130 in Human Pancreas Tissue. gp130 was detected in immersion fixed paraffin-embedded sections of human pancreas tissue using Mouse Anti-Human gp130 Monoclonal Antibody (Catalog # MAB628) at 5 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (brown; Catalog # VC001) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in exocrine cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
	Neutralization Cell Proliferation Induced by Oncostatin M/OSM and Neutralization by Human gp130 Antibody. Recombinant Human Oncostatin M/OSM stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human Oncostatin M/OSM (0.8 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human gp130 Monoclonal Antibody (Catalog # MAB628). The ND₅₀ is typically 0.02-0.1 µg/mL.

Formulation, Preparation and Storage

Purification	Protein A or G purified from ascites
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: gp130

gp130, also known as CD130, belongs to the class I cytokine receptor family. It functions as a signal transducing receptor component in the receptor complexes of the IL-6 family of cytokines including IL-6, IL-11, LIF, OSM, CNTF and CT-1.

Item	Value
Long Name	Glycoprotein 130
Entrez Gene IDs	3572 (Human); 16195 (Mouse); 25205 (Rat); 101926138 (Cynomolgus Monkey)
Alternate Names	CD130, CD130 antigen, CDw130, DKFZp564F053, IL-6 receptor subunit beta, IL-6R subunit beta, IL-6R-beta, IL-6RB, IL6ST, Interleukin-6 signal transducer, Membrane glycoprotein 130, Oncostatin-M receptor subunit alpha, gp130, gp130 of the rheumatoid arthritis antigenic peptide-bearing soluble form, interleukin 6 signal transducer (gp130, oncostatin M receptor), interleukin receptor beta chain, interleukin-6 receptor subunit beta, membrane glycoprotein gp130

Citations for Human gp130 Antibody (1)

Showing 1 - 1 of 1 citation Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Human gp130 Antibody include:

Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr

Abir R et al. (2004), Immunocytochemical detection and RT-PCR expression of leukaemia inhibitory factor and its receptor in human fetal and adult ovaries. Mol. Hum. Reprod., 10(5):313-9.
PMID: 15044601

There are no citations that match your criteria.

Showing 1 - 1 of 1 citation Showing All

Customer Reviews for Human gp130 Antibody (4)

4 out of 5

4 customer ratings

5 stars

25%

4 stars

50%

3 stars

25%

2 stars

1 star

Customer Images

Showing 1 - 4 of 4 reviews Showing All

Filter By:

Human gp130 Antibody

Name: Anonymous

Application: Microarrays

Sample Tested: EDTA Plasma

Species: Human

Verified Customer | Posted 06/10/2020
Human gp130 Antibody

Name: Anonymous

Application: MiAr

Sample Tested: EDTA Plasma

Species: Human

Verified Customer | Posted 01/09/2019
Human gp130 Antibody

Name: Anonymous

Application: ELISA

Sample Tested: Plasma,Serum

Species: Human and Mouse

Verified Customer | Posted 11/08/2018
Human gp130 Antibody

Name: Anonymous

Application: Microarrays

Sample Tested: EDTA Plasma

Species: Human

Verified Customer | Posted 11/07/2018

There are no reviews that match your criteria.

Showing 1 - 4 of 4 reviews Showing All

Product Documents for Human gp130 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Human gp130 Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Customers also Viewed

Customers also Purchased

Applications for Human gp130 Antibody

Human gp130 Sandwich Immunoassay

Scientific Data Examples for Human gp130 Antibody

Immunohistochemistry

Neutralization

Formulation, Preparation and Storage

Reconstitution Calculator

Background: gp130

Citations for Human gp130 Antibody (1)

Customer Reviews for Human gp130 Antibody (4)

Customer Images

Human gp130 Antibody

Human gp130 Antibody

Human gp130 Antibody

Human gp130 Antibody

Product Documents for Human gp130 Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

Bio-Techne Brands

Stay Connected