Human GITR/TNFRSF18 Antibody

Name: Human GITR/TNFRSF18 Antibody
Brand: R&D Systems
SKU: MAB689
Rating: 4 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB689

Citations (9)

(1)

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Key Product Details

Species Reactivity:	Human
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human GITR/TNFRSF18 Gln26-Glu161 (Thr43Ala) Accession # Q9Y5U5
Applications:	Block/Neutralize, CyTOF-ready, ELISA Capture, Flow Cytometry, Western Blot
Label:	Unconjugated
Source:	Monoclonal Mouse IgG₁ Clone # 110416
Specificity:	Detects human GITR in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) 4‑1BB, recombinant mouse (rm) 4‑1BB, rhCD27, rmCD27, rhCD30, rmCD30, rhCD40, rmCD40, rhDR3, rhDR6, rhEDAR, rmEDAR, rhFas, rmFAS, rmGITR, rhHVEM, rhLymphotoxin R beta, rmLymphotoxin R beta, rhNGF R, rhOPG, rmOPG, rhRANK, rmRANK, rhTAJ, rhTNF RI, rmTNF RI, rhTNF RII, or rmTNF RII.

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Applications for Human GITR/TNFRSF18 Antibody

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Human GITR/TNFRSF18 Fc Chimera (Catalog # 689-GR) under non-reducing conditions only
Flow Cytometry	2.5 µg/10⁶ cells	Human peripheral blood CD4⁺T cells treated with PHA
Blockade of Receptor-ligand Interaction	In a functional ELISA, 1-2 µg/mL of this antibody will block 50% of the binding of 10 ng/mL of Recombinant Human GITR Ligand/TNFSF18 (Catalog # 694-GL) to immobilized Recombinant Human GITR/TNFRSF18 Fc Chimera (Catalog # 689-GR) coated at 2 µg/mL (100 µL/well). At 5 µg/mL, this antibody will block >90% of the binding.
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Human GITR/TNFRSF18 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human GITR/TNFRSF18 Biotinylated Antibody (Catalog # BAF689)

Standard: Recombinant Human GITR/TNFRSF18 Fc Chimera Protein, CF (Catalog # 689-GR)

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: GITR/TNFRSF18

GITR (glucocorticoid-induced tumor necrosis factor receptor, also named AITR, activation-inducible TNF R family member), is a 228 amino acid (aa) type I transmembrane protein belonging to the TNF R family and has been designated TNFRSF18. The GITR cytoplasmic domain has striking homology with the cytoplasmic domain of 4-1BB and CD27. Human GITR shares 55% homology with murine GITR. GITR is expressed at low levels in peripheral blood T cells, bone marrow, thymus, spleen, and lymph nodes. In contrast to mouse GITR, expression of human GITR is not induced by treatment with dexamethasone, but is
up-regulated by antigen stimulation or by treatment with anti-CD3 plus anti-CD28, or PMA plus ionomycin. Human GITR ligand was identified from human umbilical vein endothelial cells and is a 177 aa polypeptide belonging to the TNF superfamily (TNFSF18). Ligation of GITR can activate NF-kappa B through TRAF2, and protect T cells from TCR activation-induced cell death. It has been proposed that GITR ligand and GITR may modulate T lymphocyte functions.

Item	Value
References	Nocentini, G. et al. (1997) Proc. Natl. Acad. Sci. USA 94:6216. Kwon, B. et al. (1999) J. Biol. Chem. 274:6056. Gurney, A.L. et al. (1999) Current Biology 9:215. Kwon, B. et al. (1999) Current Opinion in Immunology 11:340.
Long Name	Glucocorticoid Induced TNF Receptor Family Related Gene
Entrez Gene IDs	8784 (Human); 21936 (Mouse); 500598 (Rat); 102146362 (Cynomolgus Monkey)
Alternate Names	AITR, AITRTNF receptor superfamily activation-inducible protein, Activation-inducible TNFR family receptor, CD357, CD357 antigen, GITR, GITR-D, GITRtumor necrosis factor receptor superfamily member 18, Glucocorticoid-induced TNFR-related protein, TNFRSF18, tumor necrosis factor receptor superfamily, member 18

Citations for Human GITR/TNFRSF18 Antibody (9)

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Citations are publications that use Bio-Techne products. Selected citations for Human GITR/TNFRSF18 Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: ICC

A Heine et al. (2017), Generation and functional characterization of MDSC-like cells Oncoimmunology, 6(4):e1295203.
PMID: 28507805
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Lee F et al. (2014), The effects of CAMPATH-1H on cell viability do not correlate to the CD52 density on the cell surface. PLoS ONE, 9(7):e103254.
PMID: 25050704
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Schmiedel et al. (2013), Generation and preclinical characterization of a Fc-optimized GITR-Ig fusion protein for induction of NK cell reactivity against leukemia. Mol Ther, 21(4):877-86.
PMID: 23380816
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Mandapathil M et al. (2012), CD26 expression and adenosine deaminase activity in regulatory T cells (Treg) and CD4(+) T effector cells in patients with head and neck squamous cell carcinoma. Oncoimmunology, 1(5):659-669.
PMID: 22934258
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Savage ND et al. (2008), Human anti-inflammatory macrophages induce Foxp3+ GITR+ CD25+ regulatory T cells, which suppress via membrane-bound TGFbeta-1. J. Immunol., 181(3):2220-6.
PMID: 18641362
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Baltz KM et al. (2007), Cancer immunoediting by GITR (glucocorticoid-induced TNF-related protein) ligand in humans: NK cell/tumor cell interactions. FASEB J., 21(10):2442-54.
PMID: 17360848
Species: Human
Sample Types: Cell Culture Supernates
Applications: Neutralization

Godfrey WR et al. (2005), Cord blood CD4(+)CD25(+)-derived T regulatory cell lines express FoxP3 protein and manifest potent suppressor function. Blood, 105(2):750-8.
PMID: 15374887
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Birebent B et al. (2004), Suppressive properties of human CD4+CD25+ regulatory T cells are dependent on CTLA-4 expression. Eur. J. Immunol., 34(12):3485-96.
PMID: 15484187
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Zheng SG et al. (2004), Natural and induced CD4+CD25+ cells educate CD4+CD25- cells to develop suppressive activity: the role of IL-2, TGF-beta, and IL-10. J. Immunol., 172(9):5213-21.
PMID: 15100259

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Customer Reviews for Human GITR/TNFRSF18 Antibody (1)

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Human GITR/TNFRSF18 Antibody

Name: Paul Fisher

Application: Flow

Sample Tested: Transfected cells

Species: Human

Verified Customer | Posted 06/29/2016

looked for expression of GITR on a tranfected overexpressing cell line and it worked well versus an isotype control by flow cytometry

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Product Documents for Human GITR/TNFRSF18 Antibody

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Human GITR/TNFRSF18 Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Complete Your Research

Complete Your Experiment

Applications for Human GITR/TNFRSF18 Antibody

Human GITR/TNFRSF18 Sandwich Immunoassay

Formulation, Preparation and Storage

Reconstitution Calculator

Background: GITR/TNFRSF18

Citations for Human GITR/TNFRSF18 Antibody (9)

Customer Reviews for Human GITR/TNFRSF18 Antibody (1)

Human GITR/TNFRSF18 Antibody

Product Documents for Human GITR/TNFRSF18 Antibody

Product Datasheets

COA

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SDS

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