Human GATA-3 Antibody

Name: Human GATA-3 Antibody
Brand: R&D Systems
SKU: AF2605
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF2605

Citations (20)

(1)

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Setting the standard in quality research reagents

Key Product Details

Species Reactivity:	Human
Immunogen:	E. coli-derived recombinant human GATA-3 Pro135-Ser258 Accession # P23771
Applications:	Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Simple Western, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects human and mouse GATA-3 in direct ELISAs and Western blots.

View Full Technical Details for Human GATA-3 Antibody

Applications for Human GATA-3 Antibody

	Recommended Concentration	Sample
Western Blot	0.25 µg/mL	See below
Simple Western	2.5 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	See below
Immunocytochemistry	5-15 µg/mL	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human GATA-3 Antibody

	Western Blot Detection of Human and Mouse GATA‑3 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, Jurkat human acute T cell leukemia cell line, and EL-4 mouse lymphoblast cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human GATA-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2605) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for GATA-3 full length (FL) at approximately 55 kDa and the splice form (SF) at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Immunohistochemistry GATA‑3 in Human Breast Cancer Tissue. GATA-3 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human GATA-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2605) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
	Immunocytochemistry GATA‑3 in MCF-7 Human Cell Line. GATA-3 was detected in immersion fixed MCF-7 human breast cancer cell line using 10 µg/mL Goat Anti-Human GATA-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2605) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
	Immunohistochemistry GATA‑3 in Human Breast Cancer Tissue. GATA-3 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human GATA-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2605) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
	Simple Western Detection of Human GATA‑3 by Simple Western^TM. Simple Western lane view shows lysates of MCF-7 human breast cancer cell line and Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. Specific bands were detected for GATA-3 full length (FL) at approximately 62 kDa and the splice form (SF) at approximately 52 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Human GATA-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2605) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: GATA-3

GATA-3 belongs to the GATA family of transcription factors, which bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue-specific programs of gene expression and morphogenesis. It is widely expressed in mesodermal- and endodermal-derived tissues. GATA-3 has been shown to be an essential regulator for immune cell function, sympathetic neuron development and the maintenance of the differentiated state in epithelial cells.

Item	Value
Entrez Gene IDs	2625 (Human); 14462 (Mouse)
Alternate Names	131320, , , , , , , , ,

Citations for Human GATA-3 Antibody (20)

Showing 1 - 10 of 20 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human GATA-3 Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

M Ohgushi et al. (2022), Delamination of trophoblast-like syncytia from the amniotic ectodermal analogue in human primed embryonic stem cell-based differentiation model Cell Reports, 39(12):110973.
PMID: 35732132
Species: Human
Sample Types: Organoids
Applications: IHC

K Hiratsuka et al. (2022), Organoid-on-a-chip model of human ARPKD reveals mechanosensing pathomechanisms for drug discovery Science Advances, 8(38):eabq0866.
PMID: 36129975
Species: Human
Sample Types: Embryo
Applications: IHC

D Meisterman et al. (2021), Integrated pseudotime analysis of human pre-implantation embryo single-cell transcriptomes reveals the dynamics of lineage specification Cell Stem Cell, 0(0).
PMID: 34004179
Species: Human
Sample Types: Whole Cells
Applications: ICC

AI Yu et al. (2020), Gut Microbiota Modulate CD8ï¿½T Cell Responses to Influence Colitis-Associated Tumorigenesis Cell Rep, 31(1):107471.
PMID: 32268087
Species: Human
Sample Types: Whole Cells
Applications: ICC

MN Shahbazi et al. (2020), Developmental potential of aneuploid human embryos cultured beyond implantation Nat Commun, 11(1):3987.
PMID: 32778678
Species: Transgenic Human
Sample Types: Organoid
Applications: IHC

SE Howden et al. (2020), Plasticity of distal nephron epithelia from human kidney organoids enables the induction of ureteric tip and stalk Cell Stem Cell, 0(0).
PMID: 33378647
Species: Human
Sample Types: Whole Cells
Applications: IHC

K Uchimura et al. (2020), Human Pluripotent Stem Cell-Derived Kidney Organoids with Improved Collecting Duct Maturation and Injury Modeling Cell Reports, 33(11):108514.
PMID: 33326782
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Z Tan et al. (2020), Mouse Embryonic Stem Cell-Derived Ureteric Bud Progenitors Induce Nephrogenesis Cells, 9(2).
PMID: 32023845
Species: Human
Sample Types: Whole Cells
Applications: ICC

MN Shahbazi et al. (2020), Developmental potential of aneuploid human embryos cultured beyond implantation Nat Commun, 11(1):3987.
PMID: 32778678
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

B Phipson et al. (2019), Evaluation of variability in human kidney organoids Nat. Methods, 16(1):79-87.
PMID: 30573816
Species: Human
Sample Types: Organoids
Applications: IHC

F Schutgens et al. (2019), Tubuloids derived from human adult kidney and urine for personalized disease modeling Nat. Biotechnol., 37(3):303-313.
PMID: 30833775
Species: Mouse
Sample Types: Cell Lysates
Applications: ChIP

S Nomura et al. (2019), Pyrrothiogatain acts as an inhibitor of GATA family proteins and inhibits Th2 cell differentiation in vitro Sci Rep, 9(1):17335.
PMID: 31758034
Species: Human
Sample Types: Trophoblast Organoids
Applications: IHC

MY Turco et al. (2018), Trophoblast organoids as a model for maternal-fetal interactions during human placentation Nature, 0(0).
PMID: 30487605
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

P Shahi et al. (2017), GATA3 targets semaphorin 3B in mammary epithelial cells to suppress breast cancer progression and metastasis Oncogene, 0(0).
PMID: 28581515
Species: Human
Sample Types: Whole Cells
Applications: IHC-P

Tabata T et al. (2015), Human cytomegalovirus infection interferes with the maintenance and differentiation of trophoblast progenitor cells of the human placenta. J Virol, 89(9):5134-47.
PMID: 25741001
Species: Human
Sample Types: Whole Tissue
Applications: IHC

Takasato M et al. (2015), Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis. Nature, 526(7574):564-8.
PMID: 26444236
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Yu et al. (2013), A Gata3-Mafb transcriptional network directs post-synaptic differentiation in synapses specialized for hearing. Elife, 2(0):e01341.
PMID: 24327562
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Pellegrino MJ et al. (2011), Cytokines inhibit norepinephrine transporter expression by decreasing Hand2. Mol. Cell. Neurosci., 46(3):671-80.
PMID: 21241805
Species: Human
Sample Types: Whole Cells
Applications: ICC

Song H et al. (2009), Critical role for GATA3 in mediating Tie2 expression and function in large vessel endothelial cells. J. Biol. Chem., 284(42):29109-24.
PMID: 19674970
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Ren Y et al. (2008), Novel immunomodulatory properties of berbamine through selective down-regulation of STAT4 and action of IFN-gamma in experimental autoimmune encephalomyelitis. J. Immunol., 181(2):1491-8.
PMID: 18606704

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Human GATA-3 Antibody

Name: Alice Jouneau

Application: ICC/IF

Sample Tested: blastocyst

Species: Bovine

Verified Customer | Posted 08/09/2022

use at 10 µg/ml

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Product Documents for Human GATA-3 Antibody

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Human GATA-3 Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Applications for Human GATA-3 Antibody

Scientific Data Examples for Human GATA-3 Antibody

Western Blot

Immunohistochemistry

Immunocytochemistry

Immunohistochemistry

Simple Western

Formulation, Preparation and Storage

Reconstitution Calculator

Background: GATA-3

Citations for Human GATA-3 Antibody (20)

Customer Reviews for Human GATA-3 Antibody (1)

Customer Images

Human GATA-3 Antibody

Product Documents for Human GATA-3 Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

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