Human GATA‑3 Biotinylated Antibody

Name: Human GATA‑3 Biotinylated Antibody
Brand: R&D Systems
SKU: BAF2605

Catalog # BAF2605 | R&D Systems, Inc. a Bio-Techne Brand

Citations (1)

Product Datasheet / COA / SDS

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Product Summary
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry, Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG

Product Summary for Human GATA‑3 Biotinylated Antibody

Immunogen

E. coli-derived recombinant human GATA-3
Pro135-Ser258
Accession # P23771

Specificity

Detects human GATA-3 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human GATA‑3 Biotinylated Antibody

GATA‑3 in MCF-7 Human Cell Line.

GATA-3 was detected in immersion fixed MCF-7 human breast cancer cell line using 10 µg/mL Goat Anti-Human GATA-3 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2605) for 3 hours at room temperature. Cells were stained with the Northern-Lights™ 557-conjugated Streptavidin (red, upper panel; Catalog # NL999) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human GATA‑3 Biotinylated Antibody

Application

Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed MCF-7 human breast cancer cell line

Western Blot

0.1 µg/mL
Sample: Recombinant Human GATA‑3

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 1 publication using BAF2605 in the following applications:

IHC-P (1 Publication)

Formulation, Preparation and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GATA-3

GATA-3 belongs to the GATA family of transcription factors, which bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue-specific programs of gene expression and morphogenesis. It is widely expressed in mesodermal- and endodermal-derived tissues. GATA-3 has been shown to be an essential regulator for immune cell function, sympathetic neuron development and the maintenance of the differentiated state in epithelial cells.

Alternate Names

GATA3

Entrez Gene IDs

2625 (Human); 14462 (Mouse)

Gene Symbol

GATA3

UniProt

P23771

Product Documents for Human GATA‑3 Biotinylated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Human GATA‑3 Biotinylated Antibody

For research use only

Citations for Human GATA‑3 Biotinylated Antibody (1)

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Citations are publications that use Bio-Techne products. Selected citations for Human GATA‑3 Biotinylated Antibody include:

Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

Mansfield AS et al. (2010-12-10), Regional immunity in melanoma: immunosuppressive changes precede nodal metastasis. Mod. Pathol., 2010-124(4):487-94.
PMID: 21151098

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