Human Galectin-3 Antibody

Name: Human Galectin-3 Antibody
Brand: R&D Systems
SKU: AF1154
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF1154

Citations (9)

(1)

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Species Reactivity:	Human
Immunogen:	E. coli-derived recombinant human Galectin‑3 Ala2-Ile250 Accession # P17931.5
Applications:	CyTOF-ready, Flow Cytometry, Immunohistochemistry, Simple Western, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects human Galectin-3 in direct ELISAs and Western blots.

View Full Technical Details for Human Galectin-3 Antibody

Applications for Human Galectin-3 Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	See below
Simple Western	10 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry	2.5 µg/10⁶ cells	Human PBMCs fixed with paraformaldehyde and permeabilized with saponin

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human Galectin-3 Antibody

	Western Blot Detection of Human Galectin‑3 by Western Blot. Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line, MCF-7 human breast cancer cell line, and U-118-MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human Galectin-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1154) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Galectin-3 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Simple Western Detection of Human Galectin‑3 by Simple Western^TM. Simple Western lane view shows lysates of COLO 205 human colorectal adenocarcinoma cell line, MCF-7 human breast cancer cell line, and U-118-MG human glioblastoma/astrocytoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Galectin-3 at approximately 37-38 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Galectin-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1154) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
	Immunohistochemistry Galectin‑3 in Human Colon. Galectin-3 was detected in formalin fixed paraffin-embedded sections of human colon using Goat Anti-Human Galectin-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1154) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; (CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: Galectin-3

The galectins constitute a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified. The galectins have been classified into the prototype galectins (-1, -2, -5, -7, -10, -11, -13, -14), which contain one CRD and exist either as a monomer or a noncovalent homodimer; the chimera galectins (Galectin-3) containing one CRD linked to a nonlectin domain; and the tandem-repeat galectins (-4, -6, -8, -9, -12) consisting of two CRDs joined by a linker peptide. Galectins lack a classical signal peptide and can be localized to the cytosolic compartments where they have intracellular functions. However, via one or more as yet unidentified non-classical secretory pathways, galectins can also be secreted to function extracellularly. Individual members of the galectin family have different tissue distribution profiles and exhibit subtle differences in their carbohydrate-binding specificities. Each family member may preferentially bind to a unique subset of cell-surface glycoproteins (1-4). Galectin-3, also known as Mac-2, L29, CBP35, and epsilon BP, is a chimera galectin that has a tendency to dimerize. Besides the soluble protein, alternatively spliced forms of chicken Galectin-3 containing a transmembrane-spanning domain and a leucine zipper motif have been reported. Galectin-3 is expressed in tumor cells, macrophages, activated T cells, osteoclasts, epithelial cells, and fibroblasts. It binds various matrix glycoproteins including laminin, fibronectin, LAMPS, 90K/Mac-2BP, MP20, and CEA. Galectin-3 promotes cell growth and proliferation for many cell types. Galectin-3 acts intracellularly to prevent apoptosis. Depending on the cell types, Galectin-3 exhibits pro- or anti-adhesive properties. Galectin-3 has proinflammatory activities in vitro and in vivo. It induces pro-inflammatory and inhibits Th2 type cytokine production. Galectin-3 chemoattracts monocytes and macrophages. It activates and degranulates basophils and mast cells. Elevated circulating levels of Galectin-3 has been show to correlate with the malignant potential of several types of cancer, suggesting that Galectin-3 is also involved in tumor growth and metastasis. Human and mouse Galectin-3 shares approximately 80% amino acid sequence similarity (1-5).

Item	Value
References	Rabinovich, A. et al. (2002) Trends in Immunol. 23:313. Rabinovich, A. et al. (2002) J. Leukocyte Biology 71:741. Hughes, R.C. (2001) Biochimie 83:667. R&D Systems Cytokine Bulletin; Summer 2002. Gorski, J.P. et al. (2002) J. Biol. Chem. 277:18840.
Entrez Gene IDs	3958 (Human); 16854 (Mouse)
Alternate Names	AGE-R3, CBP 35,35 kDa lectin, CBP35, Carbohydrate-binding protein 35, GAL3, GALBPCBP35, GALIG, Gal-3, Galactose-specific lectin 3, Galactoside-binding protein, Galectin-3, Galectin3, IgE-binding protein, L-31, L29, L31, LGALS2, LGALS3, Laminin-binding protein, Lectin L-29, MAC2GAL3, Mac-2, Mac-2 antigen, galactin-3, lectin, galactoside-binding, soluble, 3

Citations for Human Galectin-3 Antibody (9)

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Citations are publications that use Bio-Techne products. Selected citations for Human Galectin-3 Antibody include:

Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

D Zheng et al. (2023), Biomimetic nanoparticles drive the mechanism understanding of shear-wave elasticity stiffness in triple negative breast cancers to predict clinical treatment Bioactive materials, 22(0):567-587.
PMID: 36382024
Species: Human
Sample Types: Serum
Applications: ELISA Capture

N Freitag et al. (2020), Galectin-3 deficiency in pregnancy increases the risk of fetal growth restriction (FGR) via placental insufficiency Cell Death Dis, 11(7):560.
PMID: 32703931
Species: Human
Sample Types: Protein
Applications: Western Blot

M Huang et al. (2020), Network analysis of the progranulin-deficient mouse brain proteome reveals pathogenic mechanisms shared in human frontotemporal dementia caused by GRN mutations Acta Neuropathol Commun, 8(1):163.
PMID: 33028409
Species: Human
Sample Types: Basement Membrane Extract
Applications: ELISA Development (Detection)

Ž Boji?-Trbo et al. (2019), Human trophoblast requires galectin-3 for cell migration and invasion Sci Rep, 9(1):2136.
PMID: 30765738
Species: Human
Sample Types: Whole Tissue
Applications: IHC

T Shimura et al. (2017), Intranuclear accumulation of galectin-3 is an independent prognostic factor for patients with distal cholangiocarcinoma Oncol Lett, 14(1):819-829.
PMID: 28693238
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Ryu K et al. (2014), Tonsil-derived mesenchymal stem cells alleviate concanavalin A-induced acute liver injury. Exp Cell Res, 326(1):143-54.
PMID: 24954408
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Delgado VM et al. (2011), Modulation of endothelial cell migration and angiogenesis: a novel function for the "tandem-repeat" lectin galectin-8. FASEB J., 25(1):242-54.
PMID: 20876211
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Thijssen VL et al. (2008), The galectin profile of the endothelium: altered expression and localization in activated and tumor endothelial cells. Am. J. Pathol., 172(2):545-53.
PMID: 18202194
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Zhuo Y et al. (2008), Sialylation of beta1 integrins blocks cell adhesion to galectin-3 and protects cells against galectin-3-induced apoptosis. J. Biol. Chem., 283(32):22177-85.
PMID: 18676377

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Customer Reviews for Human Galectin-3 Antibody (1)

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Human Galectin-3 Antibody

Name: Anonymous

Application: ICC/IF

Sample Tested: RAW 264.7 mouse monocyte/macrophage cell line

Species: Mouse

Verified Customer | Posted 11/18/2016

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Human Galectin-3 Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Human Galectin-3 Antibody

Scientific Data Examples for Human Galectin-3 Antibody

Western Blot

Simple Western

Immunohistochemistry

Formulation, Preparation and Storage

Reconstitution Calculator

Background: Galectin-3

Citations for Human Galectin-3 Antibody (9)

Customer Reviews for Human Galectin-3 Antibody (1)

Customer Images

Human Galectin-3 Antibody

Product Documents for Human Galectin-3 Antibody

Product Datasheets

COA

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