Human Galectin-3 Biotinylated Antibody

Name: Human Galectin-3 Biotinylated Antibody
Brand: R&D Systems
SKU: BAF1154
Rating: 5 (2 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # BAF1154

Citations (4)

(2)

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Key Product Details

Species Reactivity:	Human
Immunogen:	E. coli-derived recombinant human Galectin‑3 Ala2-Ile250 Accession # Q6IBA7
Applications:	ELISA Detection, Flow Cytometry, Western Blot
Label:	Biotin
Source:	Polyclonal Goat IgG
Specificity:	Detects human Galectin‑3 in ELISAs and Western blots. In sandwich ELISAs, approximately 25% cross-reactivity with recombinant mouse Galectin‑3 is observed and less than 0.2% cross-reactivity with recombinant human Galectin‑1, -2, -4, -7, -8, and -10 is observed.

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Applications for Human Galectin-3 Biotinylated Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human Galectin‑3 (Catalog # 1154-GA)
Flow Cytometry	2.5 µg/10⁶ cells	Human PBMCs fixed with paraformaldehyde and permeabilized with saponin

Human Galectin-3 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Human Galectin‑3 Antibody (Catalog # MAB11541)

Standard: Recombinant Human Galectin-3 Protein (Catalog # 1154-GA)

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: Galectin-3

The galectins constitute a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified. The galectins have been classified into the prototype galectins (-1, -2, -5, -7, -10, -11, -13, -14), which contain one CRD and exist either as a monomer or a noncovalent homodimer; the chimera galectins (Galectin-3) containing one CRD linked to a nonlectin domain; and the tandem-repeat galectins (-4, -6, -8, -9, -12) consisting of two CRDs joined by a linker peptide. Galectins lack a classical signal peptide and can be localized to the cytosolic compartments where they have intracellular functions. However, via one or more as yet unidentified non-classical secretory pathways, galectins can also be secreted to function extracellularly. Individual members of the galectin family have different tissue distribution profiles and exhibit subtle differences in their carbohydrate-binding specificities. Each family member may preferentially bind to a unique subset of cell-surface glycoproteins (1-4).

Galectin-3, also known as Mac-2, L29, CBP35, and epsilon BP, is a chimera galectin that has a tendency to dimerize. Besides the soluble protein, alternatively spliced forms of chicken Galectin-3 containing a transmembrane-spanning domain and a leucine zipper motif have been reported. Galectin-3 is expressed in tumor cells, macrophages, activated T cells, osteoclasts, epithelial cells, and fibroblasts. It binds various matrix glycoproteins including laminin, fibronectin, LAMPS, 90K/Mac-2BP, MP20, and CEA. Galectin-3 promotes cell growth and proliferation for many cell types. Galectin-3 acts intracellularly to prevent apoptosis. Depending on the cell types, Galectin-3 exhibits pro- or anti-adhesive properties. Galectin-3 has proinflammatory activities in vitro and in vivo. It induces pro-inflammatory and inhibits Th2 type cytokine production. Galectin-3 chemoattracts monocytes and macrophages. It activates and degranulates basophils and mast cells. Elevated circulating levels of Galectin-3 has been show to correlate with the malignant potential of several types of cancer, suggesting that Galectin-3 is also involved in tumor growth and metastasis. Human and mouse Galectin-3 shares approximately 80% amino acid sequence similarity (1-5).

Item	Value
References	Rabinovich, A. et al. (2002) Trends in Immunol. 23:313. Rabinovich, A. et al. (2002) J. Leukocyte Biology 71:741. Hughes, R.C. (2001) Biochimie 83:667. R&D Systems Cytokine Bulletin; Summer 2002. Gorski, J.P. et al. (2002) J. Biol. Chem. 277:18840.
Entrez Gene IDs	3958 (Human); 16854 (Mouse)
Alternate Names	AGE-R3, CBP 35,35 kDa lectin, CBP35, Carbohydrate-binding protein 35, GAL3, GALBPCBP35, GALIG, Gal-3, Galactose-specific lectin 3, Galactoside-binding protein, Galectin-3, Galectin3, IgE-binding protein, L-31, L29, L31, LGALS2, LGALS3, Laminin-binding protein, Lectin L-29, MAC2GAL3, Mac-2, Mac-2 antigen, galactin-3, lectin, galactoside-binding, soluble, 3

Citations for Human Galectin-3 Biotinylated Antibody (4)

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Citations are publications that use Bio-Techne products. Selected citations for Human Galectin-3 Biotinylated Antibody include:

Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Detection

HM Hsu et al. (2023), Distinct features of the host-parasite interactions between nonadherent and adherent Trichomonas vaginalis isolates PloS Neglected Tropical Diseases, 17(1):e0011016.
PMID: 36595499
Species: Human
Sample Types: Serum
Applications: ELISA Detection

N Freitag et al. (2020), Galectin-3 deficiency in pregnancy increases the risk of fetal growth restriction (FGR) via placental insufficiency Cell Death Dis, 11(7):560.
PMID: 32703931
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

E Gallardo-V et al. (2019), Endoglin Protein Interactome Profiling Identifies TRIM21 and Galectin-3 as New Binding Partners Cells, 8(9).
PMID: 31540324
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Sioud M et al. (2011), Mesenchymal stem cell-mediated T cell suppression occurs through secreted galectins. Int. J. Oncol., 38(2):385-90.
PMID: 21165555

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Customer Reviews for Human Galectin-3 Biotinylated Antibody (2)

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Human Galectin‑3 Biotinylated Antibody

Name: Anonymous

Application: WB

Sample Tested: Cancer cell lysates

Species: Human

Verified Customer | Posted 10/26/2015

Specificity: SpecificSensitivity: SensitiveBuffer: Milk BSA MilkDilution: 1:1000
Human Galectin‑3 Biotinylated Antibody

Name: Anonymous

Application: IHC

Sample Tested: Colon tissue

Species: Human

Verified Customer | Posted 10/26/2015

This antibody was first tested on tissues before the mouse IgG monoclonal version, the staining is diffuse and not as good as the other clone. Specificity: Reasonably specificSensitivity: Not sensitiveBuffer: PBSDilution: 1:500

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